Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,757,006	G→A	A437T (GCT→ACT)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,757,006	0	G	A	100.0%	203.1 / NA	63	A437T (GCT→ACT)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/35);  total (28/35)

CCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTA  >  NC_000913/1756870‑1757135                                                                                                                                         |                                                                                                                                  cccGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGAct                                                                                                                                 >  2:293621/1‑139 (MQ=255)      aTGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCa                                                                                                                            <  1:493163/139‑1 (MQ=255)           accaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCg                                                                                                                       >  2:355398/1‑139 (MQ=255)           accaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCg                                                                                                                       >  2:272883/1‑139 (MQ=255)           accaTCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCg                                                                                                                       <  1:697171/139‑1 (MQ=255)                ccTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTg                                                                                                                  <  1:999948/139‑1 (MQ=255)                      aCTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGcacac                                                                                                            >  2:878867/1‑139 (MQ=255)                        tGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACaa                                                                                                          <  1:293621/139‑1 (MQ=255)                         gACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGt                                                                                                                     >  2:502897/1‑127 (MQ=255)                         gACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGt                                                                                                                     <  1:502897/127‑1 (MQ=255)                          accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAg                                                                                                        >  1:186885/1‑139 (MQ=255)                          accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAg                                                                                                        >  2:237339/1‑139 (MQ=255)                          accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAg                                                                                                        >  1:698673/1‑139 (MQ=255)                          accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAg                                                                                                        >  1:510683/1‑139 (MQ=255)                          accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAg                                                                                                        >  1:35501/1‑139 (MQ=255)                          accaccAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGATTGTTAAAGAGATCACTTCTACTGTTGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAg                                                                                                        >  1:432962/1‑139 (MQ=255)                            caccaACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGt                                                                                                      <  1:436682/139‑1 (MQ=255)                                 aCGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt                                                                                                 <  1:1043643/139‑1 (MQ=255)                                 aCGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt                                                                                                 <  2:169152/139‑1 (MQ=255)                                   gAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGATTGTTAAAGAGATCACTTCTACTGTTGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTg                                                                                               <  2:432962/139‑1 (MQ=255)                                         aCGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTa                                                                                         >  1:938965/1‑139 (MQ=255)                                                atcaGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTc                                                                                  <  1:237339/139‑1 (MQ=255)                                                   aGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCAGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTgg                                                                               <  2:938965/139‑1 (MQ=255)                                                     ttGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTg                                                                             <  1:280117/139‑1 (MQ=255)                                                       ggTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCa                                                                           >  1:88867/1‑139 (MQ=255)                                                            tGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGt                                                                      >  2:825226/1‑139 (MQ=255)                                                             gAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTTTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTa                                                                     >  2:791890/1‑139 (MQ=255)                                                              aGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCagag                                                                                                                         <  1:194466/86‑1 (MQ=255)                                                              aGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCagag                                                                                                                         >  2:194466/1‑86 (MQ=255)                                                               gCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTAcc                                                                   >  1:418633/1‑139 (MQ=255)                                                                   aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCagag                                                                                                                         <  1:893326/81‑1 (MQ=255)                                                                   aGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCagag                                                                                                                         >  2:893326/1‑81 (MQ=255)                                                                      cGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGc                                                            <  2:186885/139‑1 (MQ=255)                                                                      cGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGc                                                            <  2:35501/139‑1 (MQ=255)                                                                      cGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGc                                                            <  2:510683/139‑1 (MQ=255)                                                                        ttGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGa                                                                                                    <  2:338288/97‑1 (MQ=255)                                                                        ttGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGa                                                                                                    >  1:338288/1‑97 (MQ=255)                                                                        ttGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt                                                                                                 >  1:939639/1‑100 (MQ=255)                                                                        ttGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGt                                                                                                 <  2:939639/100‑1 (MQ=255)                                                                              cGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTaa                                                    <  1:58061/139‑1 (MQ=255)                                                                                               aTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACg                                   <  2:418633/139‑1 (MQ=255)                                                                                               aTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACg                                   <  2:88867/139‑1 (MQ=255)                                                                                               aTCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACg                                   >  2:981966/1‑139 (MQ=255)                                                                                                 cACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTc                                 >  1:194278/1‑139 (MQ=255)                                                                                                 cACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTc                                 >  2:478413/1‑139 (MQ=255)                                                                                                 cACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTc                                 <  1:414613/139‑1 (MQ=255)                                                                                                         cTGATGATTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAata                         <  2:884386/139‑1 (MQ=255)                                                                                                            atgatTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCAt                                            <  1:625015/117‑1 (MQ=255)                                                                                                            atgatTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTg                      <  1:272883/139‑1 (MQ=255)                                                                                                            atgatTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGCTGCACTGGTACCGAGCGGCACTACTAACACCGCAt                                            >  2:625015/1‑117 (MQ=255)                                                                                                              gatTTCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCt                    >  2:1346/1‑139 (MQ=255)                                                                                                                 ttCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCtttt                 <  2:278481/139‑1 (MQ=255)                                                                                                                 ttCTACCGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCtttt                 <  2:535745/139‑1 (MQ=255)                                                                                                                      ccGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTg                              >  2:436130/1‑121 (MQ=255)                                                                                                                      ccGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTg                              <  1:436130/121‑1 (MQ=255)                                                                                                                      ccGTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaa            <  1:878867/139‑1 (MQ=255)                                                                                                                        gTCTGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaatt          <  2:99124/139‑1 (MQ=255)                                                                                                                           tGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaattaat       <  2:878157/139‑1 (MQ=255)                                                                                                                           tGGGTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaattaat       <  1:748943/139‑1 (MQ=255)                                                                                                                             ggTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaatta         <  1:233338/135‑1 (MQ=255)                                                                                                                             ggTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGaatta         >  2:233338/1‑135 (MQ=255)                                                                                                                              gTAAAGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTg    >  1:248595/1‑139 (MQ=255)                                                                                                                                aaaGAACTGACTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACGCCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGta  <  1:318518/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  CCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTA  >  NC_000913/1756870‑1757135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑