Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,789,804	G→A	*64* (TAG→TAA)	ydiE →	hemin uptake protein HemP homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,789,804	0	G	A	100.0%	113.4 / NA	36	*64* (TAG→TAA)	ydiE	hemin uptake protein HemP homolog
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/17);  total (19/17)

TTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAGCCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCC  >  NC_000913/1789672‑1789934                                                                                                                                     |                                                                                                                                   ttCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTa                                                                                                                              >  1:838583/1‑139 (MQ=255)             tATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCt                                                                                                                  >  2:608083/1‑139 (MQ=255)               ttCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGa                                                                                                                >  2:425883/1‑139 (MQ=255)                     gAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCa                                                                                                          >  1:686958/1‑139 (MQ=255)                             agccagACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTa                                                                                                  <  1:419994/139‑1 (MQ=255)                                cagACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTAccc                                                                                               <  2:700001/139‑1 (MQ=255)                                cagACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTACTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTAccc                                                                                               <  2:973485/139‑1 (MQ=255)                                         ttAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGagcagc                                                                                                                   >  2:206543/1‑110 (MQ=255)                                         ttAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGagcagc                                                                                                                   <  1:206543/110‑1 (MQ=255)                                           aGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTgg                                                                                    <  2:686958/139‑1 (MQ=255)                                              tCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGgacg                                                                                 >  1:775352/1‑139 (MQ=255)                                                     ggCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                          <  2:103436/139‑1 (MQ=255)                                                           cTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGtca                                                                    <  1:774431/139‑1 (MQ=255)                                                                                        tCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGt                                                                                           >  1:318219/1‑87 (MQ=255)                                                                                        tCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGt                                                                                           <  2:318219/87‑1 (MQ=255)                                                                                           gCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGc                                    <  1:425883/139‑1 (MQ=255)                                                                                              ccGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTAc                                                                                                 <  2:145458/75‑1 (MQ=255)                                                                                              ccGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTAc                                                                                                 >  1:145458/1‑75 (MQ=255)                                                                                              ccGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTc                                 >  2:674087/1‑139 (MQ=255)                                                                                                gTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATa                                                                        <  2:947227/98‑1 (MQ=255)                                                                                                gTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATa                                                                        >  1:947227/1‑98 (MQ=255)                                                                                                 tAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGgacga                                                                                >  1:323787/1‑89 (MQ=255)                                                                                                 tAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGgacga                                                                                <  2:323787/89‑1 (MQ=255)                                                                                                 tAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATg                              >  2:109873/1‑139 (MQ=255)                                                                                                      cTCAGGCTGGCAAGCTGCTGTTGAACAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGc                         >  2:715416/1‑139 (MQ=255)                                                                                                         aGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                          >  1:730124/1‑87 (MQ=255)                                                                                                         aGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                          <  2:730124/87‑1 (MQ=255)                                                                                                         aGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCAtctc                                                             >  2:122856/1‑100 (MQ=255)                                                                                                         aGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCAtctc                                                             <  1:122856/100‑1 (MQ=255)                                                                                                         aGGCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGt                      >  1:566183/1‑139 (MQ=255)                                                                                                           gCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                          <  2:690962/85‑1 (MQ=255)                                                                                                           gCTGGCAAGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACa                                                                          >  1:690962/1‑85 (MQ=255)                                                                                                                  aGCTGCTGTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCg             <  1:109873/139‑1 (MQ=255)                                                                                                                        tgTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCatat       <  2:838583/139‑1 (MQ=255)                                                                                                                         gTTGACCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATc      >  2:64449/1‑139 (MQ=255)                                                                                                                             aCCAAGTAACCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCAccc  >  1:885329/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                   TTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAGCCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGCAACGCCTCATGCAGGCGGTGCAATTCCGTCATATCACCC  >  NC_000913/1789672‑1789934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑