Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,794,172	C→T	*327* (TAG→TAA)	btuC ←	vitamin B12 ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,794,172	0	C	T	97.1%	99.6 / NA	34	*327* (TAG→TAA)	btuC	vitamin B12 ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (23/10);  total (24/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTTCTCCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGCTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGC  >  NC_000913/1794042‑1794299                                                                                                                                   |                                                                                                                                aaCTTCTCCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTcc                                                                                                                         >  2:774311/1‑139 (MQ=255)     tctcCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCt                                                                                                                     >  2:398935/1‑139 (MQ=255)              tGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAAt                                                                                                            >  2:347199/1‑139 (MQ=255)                           gTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTC‑TTTTGCGGTTAACGTc                                                                                                                          >  1:382799/1‑111 (MQ=255)                           gTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTC‑TTTTGCGGTTAACGTc                                                                                                                          <  2:382799/111‑1 (MQ=255)                             cGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACAcc                                                                                             >  1:447092/1‑139 (MQ=255)                                                 gtcAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGt                                                                         >  1:51609/1‑139 (MQ=255)                                                    aGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGAc                                                                      <  2:950221/139‑1 (MQ=255)                                                        tGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACg                                                                  <  2:742124/139‑1 (MQ=255)                                                                tGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGt                                                                         >  1:1009305/1‑124 (MQ=255)                                                                tGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGt                                                                         <  2:1009305/124‑1 (MQ=255)                                                                       tttCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGctct                                                   >  2:461753/1‑139 (MQ=255)                                                                               gggTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACg                                                                                <  1:724429/102‑1 (MQ=255)                                                                               gggTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACg                                                                                >  2:724429/1‑102 (MQ=255)                                                                               gggTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCg                                                                           <  2:317611/107‑1 (MQ=255)                                                                               gggTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCg                                                                           >  1:317611/1‑107 (MQ=255)                                                                               gggTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGc                                           >  2:70293/1‑139 (MQ=255)                                                                                     ggTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGc                                     >  2:952215/1‑139 (MQ=255)                                                                                      gTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGcc                                    >  2:299087/1‑139 (MQ=255)                                                                                                aTCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTa                          <  1:117982/139‑1 (MQ=255)                                                                                                     aGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTGCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAata                     >  2:233707/1‑139 (MQ=255)                                                                                                      gCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAatat                    <  1:94010/139‑1 (MQ=255)                                                                                                          ttAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGc                <  1:868077/139‑1 (MQ=255)                                                                                                          ttAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGc                >  1:830014/1‑139 (MQ=255)                                                                                                                    aCCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAAt      >  1:97516/1‑139 (MQ=255)                                                                                                                    aCCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAAt      >  1:418613/1‑139 (MQ=255)                                                                                                                       gTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTa                          <  2:85996/116‑1 (MQ=255)                                                                                                                       gTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcg   >  2:339951/1‑139 (MQ=255)                                                                                                                       gTCTTTTTGCGGCTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTa                          >  1:85996/1‑116 (MQ=255)                                                                                                                        tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCCGATAAACACCGGCGCACCTACCGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc  >  2:790147/1‑139 (MQ=255)                                                                                                                        tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc  >  2:607594/1‑139 (MQ=255)                                                                                                                        tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc  >  1:675803/1‑139 (MQ=255)                                                                                                                        tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc  >  2:82662/1‑139 (MQ=255)                                                                                                                        tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc  >  1:415158/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                AACTTCTCCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGCTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGC  >  NC_000913/1794042‑1794299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑