Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,892,402	A→G	S278S (AGT→AGC)	yoaA ←	putative ATP‑dependent helicase, DinG family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,892,402	0	A	G	100.0%	117.7 / NA	33	S278S (AGT→AGC)	yoaA	putative ATP‑dependent helicase, DinG family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/16);  total (17/16)

TTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATA  >  NC_000913/1892268‑1892527                                                                                                                                       |                                                                                                                              ttCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGctct                                                                                                                           >  1:713224/1‑139 (MQ=255)     ccACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGc                                                                                                                       <  1:609413/139‑1 (MQ=255)          tCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGAc                                                                                                                  <  1:52826/139‑1 (MQ=255)                  aaGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcg                                                                                                          <  1:639222/139‑1 (MQ=255)                     ttCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCAc                                                                                                       >  2:93176/1‑139 (MQ=255)                     ttCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCAc                                                                                                       >  1:343720/1‑139 (MQ=255)                     ttCCAGGGTGTCATCGACCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCAc                                                                                                       >  1:830349/1‑139 (MQ=255)                      tCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACt                                                                                                      >  2:878021/1‑139 (MQ=255)                                cATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAActg                                                                                            <  1:837491/139‑1 (MQ=255)                                     aGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTggg                                                                                       <  1:383894/139‑1 (MQ=255)                                      gCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgt                                                                                      <  1:192114/139‑1 (MQ=255)                                      ccAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgt                                                                                      <  2:830349/138‑1 (MQ=255)                                       cAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgtg                                                                                     >  1:894258/1‑139 (MQ=255)                                       cAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGgtg                                                                                     >  1:730298/1‑139 (MQ=255)                                         gTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTc                                                                                   >  1:374230/1‑139 (MQ=255)                                          tAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCt                                                                                  >  2:209652/1‑139 (MQ=255)                                               aTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTa                                                                                                >  2:1033280/1‑120 (MQ=255)                                               aTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTa                                                                                                <  1:1033280/120‑1 (MQ=255)                                                   ccGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCg                                                                         <  1:93176/139‑1 (MQ=255)                                                    cGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCgg                                                                        >  2:596422/1‑139 (MQ=255)                                                    cGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCgg                                                                        >  1:441819/1‑139 (MQ=255)                                                      cTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTg                                                                      <  1:496458/139‑1 (MQ=255)                                                                      gCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGa                                                      <  1:596422/139‑1 (MQ=255)                                                                                         tACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAg                                   <  1:1012126/139‑1 (MQ=255)                                                                                           ccGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCa                                 >  1:871757/1‑139 (MQ=255)                                                                                                aTAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGACGTCTTTTGCCAGGTCGAGCAGTTGt                            <  2:929376/139‑1 (MQ=255)                                                                                                 tAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCgg                                                                        <  1:9773/94‑1 (MQ=255)                                                                                                 tAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCgg                                                                        >  2:9773/1‑94 (MQ=255)                                                                                                 tAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTc                           >  1:774628/1‑139 (MQ=255)                                                                                                         cTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGgag                   <  2:471066/139‑1 (MQ=255)                                                                                                              cGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtga              >  2:460818/1‑139 (MQ=255)                                                                                                                          gAAAATCCTTCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATa  <  2:374230/139‑1 (MQ=255)                                                                                                                          gAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGCCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATa  >  2:389596/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                              TTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATA  >  NC_000913/1892268‑1892527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑