Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,805	G→A	G40S (GGC→AGC)	mntP →	putative Mn(2+) efflux pump, mntR‑regulated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,805	0	G	A	100.0%	156.8 / NA	49	G40S (GGC→AGC)	mntP	putative Mn(2+) efflux pump, mntR‑regulated
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/22);  total (27/22)

CCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAA  >  NC_000913/1905670‑1905937                                                                                                                                        |                                                                                                                                     cccGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGcc                                                                                                                                   <  1:635167/139‑1 (MQ=255)          gACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTg                                                                                                                          >  2:103154/1‑139 (MQ=255)             aTTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTg                                                                                                                       <  1:234319/139‑1 (MQ=255)               tGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGcc                                                                                                                     >  2:932621/1‑139 (MQ=255)               tGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGcc                                                                                                                     >  1:454012/1‑139 (MQ=255)               tGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGcc                                                                                                                     >  1:879156/1‑139 (MQ=255)                gTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCg                                                                                                                    >  2:509902/1‑139 (MQ=255)                                cTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgcc                                                                                                    >  1:914705/1‑139 (MQ=255)                                cTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgcc                                                                                                    >  1:881685/1‑139 (MQ=255)                                      ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                              <  1:370914/139‑1 (MQ=255)                                      ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                              <  1:798462/139‑1 (MQ=255)                                        cttcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCg                                                                                            >  2:67341/1‑139 (MQ=255)                                        cttcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCg                                                                                            >  2:1037767/1‑139 (MQ=255)                                           cttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCt                                                                                         >  2:166167/1‑139 (MQ=255)                                            ttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTg                                                                                        <  1:641465/139‑1 (MQ=255)                                              gCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTggg                                                                                      >  1:909482/1‑139 (MQ=255)                                              gCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTggg                                                                                      <  1:494234/139‑1 (MQ=255)                                                gTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGGAACCCTGACGCCGCTGATCGGCTGGGGa                                                                                    >  2:302199/1‑139 (MQ=255)                                                             atggatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTag                                                                       >  1:947236/1‑139 (MQ=255)                                                              tggatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                              >  1:668311/1‑115 (MQ=255)                                                              tggatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                              <  2:668311/115‑1 (MQ=255)                                                                 atgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTagccag                                                                   <  1:1037767/139‑1 (MQ=255)                                                                  tgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTagccagc                                                                  >  2:546458/1‑139 (MQ=255)                                                                    cATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCg                                                                >  1:461319/1‑139 (MQ=255)                                                                      tttGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGt                                                              <  2:909482/139‑1 (MQ=255)                                                                            gcATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCGTTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTcc                                                        <  1:103154/139‑1 (MQ=255)                                                                                   tCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGa                                                                                                        >  2:825820/1‑84 (MQ=255)                                                                                   tCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGa                                                                                                        <  1:825820/84‑1 (MQ=255)                                                                                        aaaGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAAcc                                            <  1:932621/139‑1 (MQ=255)                                                                                            gTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTg                                        >  1:511/1‑139 (MQ=255)                                                                                             tGCCACCCTCCATAAACCGAAATTTTCTGCAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTgg                                       >  2:238445/1‑139 (MQ=255)                                                                                             tGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTgg                                       >  2:846743/1‑139 (MQ=255)                                                                                              gCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGa                                      >  1:274556/1‑139 (MQ=255)                                                                                               ccACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGt                                                              >  2:787815/1‑114 (MQ=255)                                                                                               ccACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGt                                                              <  1:787815/114‑1 (MQ=255)                                                                                                 aCCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTg                                   >  1:13057/1‑139 (MQ=255)                                                                                                     tCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAAt                                                  >  2:104317/1‑120 (MQ=255)                                                                                                     tCCATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAAt                                                  <  1:104317/120‑1 (MQ=255)                                                                                                       cATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTg                                        <  1:1033868/128‑1 (MQ=255)                                                                                                       cATAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTg                                        >  2:1033868/1‑128 (MQ=255)                                                                                                         tAAACCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTtgtg                           <  1:233537/139‑1 (MQ=255)                                                                                                          aaaCCGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGtgc                          <  2:215512/139‑1 (MQ=255)                                                                                                             ccGAAATTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGtgctgc                       >  1:443770/1‑139 (MQ=255)                                                                                                                aaaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGa                    <  2:167752/139‑1 (MQ=255)                                                                                                                 aaTTTTCTGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGat                   <  2:443770/139‑1 (MQ=255)                                                                                                                        tGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                              >  1:756467/1‑57 (MQ=255)                                                                                                                        tGAAGCATTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                              <  2:756467/57‑1 (MQ=255)                                                                                                                              aTTGCGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCggcggg      <  1:67341/139‑1 (MQ=255)                                                                                                                                  cGAACCAGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGaa  <  2:13057/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     CCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAA  >  NC_000913/1905670‑1905937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑