| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,926,096 | G→A | *219* (TAG→TAA) | yobB → | C‑N hydrolase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,926,096 | 0 | G | A | 96.8% | 190.3 / ‑2.0 | 63 | *219* (TAG→TAA) | yobB | C‑N hydrolase family protein |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (36/25); total (37/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
AAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGT > NC_000913/1925964‑1926229 | aaaTGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCtttt > 1:454864/1‑139 (MQ=255)aaaTGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTCCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCTTTACGCTAAGCtttt < 2:704590/139‑1 (MQ=255) aaTGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCttttt < 1:728800/139‑1 (MQ=255) aTGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTc > 2:169665/1‑139 (MQ=255) aTGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTc > 1:974884/1‑139 (MQ=255) ccGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGTGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc < 2:470360/139‑1 (MQ=255) ccGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc < 2:39036/139‑1 (MQ=255) ccGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc < 2:451404/139‑1 (MQ=255) cACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGCTCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg > 2:573670/1‑139 (MQ=255) cACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCttttt < 2:818184/120‑1 (MQ=255) cACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCttttt > 1:818184/1‑120 (MQ=255) gggATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTGCGGCCTGGAGCATGCCATGTTgcgc < 2:454864/139‑1 (MQ=255) gATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCAt > 2:787463/1‑139 (MQ=255) atatGGTCGTCTTATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa < 1:751013/116‑1 (MQ=255) atatGGTCGTCTTATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGa > 2:751013/1‑116 (MQ=255) atatGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCg < 1:1014099/139‑1 (MQ=255) ggTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATAc > 2:86320/1‑139 (MQ=255) gtcgtcTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACa < 1:704771/139‑1 (MQ=255) gtcgtcTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACa > 2:257716/1‑139 (MQ=255) tcgtcTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg < 1:596049/120‑1 (MQ=255) tcgtcTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg > 2:596049/1‑120 (MQ=255) tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGC‑AGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc > 2:259918/1‑139 (MQ=255) aTCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg < 1:740793/113‑1 (MQ=255) aTCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg > 2:740793/1‑113 (MQ=255) aTCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGa > 2:85903/1‑127 (MQ=255) aTCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGa < 1:85903/127‑1 (MQ=255) aTCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTg > 1:260942/1‑139 (MQ=255) cGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCg < 1:86320/139‑1 (MQ=255) gTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCgg > 1:14235/1‑139 (MQ=255) ttCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGt < 1:169665/139‑1 (MQ=255) aTCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCaggga > 2:522244/1‑139 (MQ=255) aTCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAACTGCGGTTTGCaggga > 1:949497/1‑139 (MQ=255) tCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGC‑AGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggagg < 1:259918/139‑1 (MQ=255) ttattGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTga > 2:717504/1‑139 (MQ=255) gTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACa < 1:505953/102‑1 (MQ=255) gTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACa > 2:505953/1‑102 (MQ=255) aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCt < 2:164878/106‑1 (MQ=255) aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCt > 1:164878/1‑106 (MQ=255) aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct < 1:571076/139‑1 (MQ=255) cGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGAt > 2:310027/1‑131 (MQ=255) cGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGAt < 1:310027/131‑1 (MQ=255) gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCg < 2:629262/122‑1 (MQ=255) gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCg > 1:629262/1‑122 (MQ=255) gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct > 2:197234/1‑134 (MQ=255) gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct < 1:197234/134‑1 (MQ=255) gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGtt > 1:695871/1‑139 (MQ=255) tCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGAGCGTTGAGATTGCCTCTGTTg > 2:257240/1‑139 (MQ=255) ggTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGaaa > 2:324898/1‑139 (MQ=255) gTTGGCAAGGCAATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATACCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGaaaa > 2:612678/1‑139 (MQ=255) ttGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct > 2:735910/1‑115 (MQ=255) ttGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct < 1:735910/115‑1 (MQ=255) gCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGc < 1:256260/110‑1 (MQ=255) gCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGc > 2:256260/1‑110 (MQ=255) aGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCa > 1:8333/1‑139 (MQ=255) ggCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCaa < 2:366401/139‑1 (MQ=255) gCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAc > 1:1052710/1‑139 (MQ=255) tCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGcc > 2:915761/1‑139 (MQ=255) cATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg < 2:514989/37‑1 (MQ=255) cATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg > 1:514989/1‑37 (MQ=255) ttACGCTAAGCTTTTACGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGACTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCt > 2:704894/1‑139 (MQ=255) aCGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGAATTATCGATACAGAAACATGCGGCTTGCAGGGAGGGATCGCAGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAACCCATAAGCAACCTgg > 2:35153/1‑139 (MQ=255) cGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGt > 2:484083/1‑139 (MQ=255) cGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGt > 2:392777/1‑139 (MQ=255) | AAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGT > NC_000913/1925964‑1926229 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |