Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,010,745	T→C	F49S (TTC→TCC)	yedL →	GNAT family putative N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,010,745	0	T	C	100.0%	152.1 / NA	43	F49S (TTC→TCC)	yedL	GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/21);  total (22/21)

TAAAACAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGATT  >  NC_000913/2010611‑2010875                                                                                                                                       |                                                                                                                                   tAAAACAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTgg                                                                                                                                >  1:100594/1‑139 (MQ=255)  aaaaCAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGt                                                                                                                               <  2:73371/139‑1 (MQ=255)  aaaaCAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGt                                                                                                                               <  2:717397/139‑1 (MQ=255)  aaaaCAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGt                                                                                                                               <  1:483980/139‑1 (MQ=255)  aaaaCAGATGATCTCACCCATCCAGCAGTGCAAGCATTAATGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGt                                                                                                                               <  2:897722/139‑1 (MQ=255)        gatgatCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGt                                                                                                                               <  2:963158/133‑1 (MQ=255)        gatgatCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGt                                                                                                                               >  1:963158/1‑133 (MQ=255)          tgatCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATgg                                                                                                                       >  1:312808/1‑139 (MQ=255)                 aCCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCT‑CCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGt                                                                                                                               <  1:223506/123‑1 (MQ=255)                 aCCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCT‑CCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGt                                                                                                                               >  2:223506/1‑123 (MQ=255)                                   gCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGt                                                                                                                               >  2:149280/1‑106 (MQ=255)                                   gCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGt                                                                                                                               <  1:149280/106‑1 (MQ=255)                                     aTTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGt                                                                                            >  1:92257/1‑139 (MQ=255)                                        aGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGt                                                                                                                               >  2:892356/1‑101 (MQ=255)                                        aGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGt                                                                                                                               <  1:892356/101‑1 (MQ=255)                                        aGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTgcg                                                                                         >  1:81453/1‑139 (MQ=255)                                               tACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGt                                                                                  >  1:809785/1‑139 (MQ=255)                                                           ggCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATa                                                                      >  1:700472/1‑139 (MQ=255)                                                                 ctgcAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACg                                                                >  1:765687/1‑139 (MQ=255)                                                                 ctgcAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACg                                                                >  1:758769/1‑139 (MQ=255)                                                                 ctgcAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACg                                                                <  2:809785/139‑1 (MQ=255)                                                                 ctgcAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACg                                                                <  2:446303/139‑1 (MQ=255)                                                                   gcagcaGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGc                                                              <  2:312808/139‑1 (MQ=255)                                                                      gcagTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAg                                                           >  2:471577/1‑139 (MQ=255)                                                                          tctcCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCa                                                                                                     <  1:652910/93‑1 (MQ=255)                                                                          tctcCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCa                                                                                                     >  2:652910/1‑93 (MQ=255)                                                                          tctcCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGa                                                       >  1:487953/1‑139 (MQ=255)                                                                                  cGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACGTCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATg                                               <  2:765687/139‑1 (MQ=255)                                                                                          gTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACcgc                                       >  1:202384/1‑139 (MQ=255)                                                                                                cTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCaaa                                 <  2:153781/139‑1 (MQ=255)                                                                                                       tgtgCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTa                          <  2:758769/139‑1 (MQ=255)                                                                                                         tgCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAAt                                                >  2:844257/1‑115 (MQ=255)                                                                                                         tgCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAAt                                                <  1:844257/115‑1 (MQ=255)                                                                                                         tgCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTAcg                        >  1:53778/1‑139 (MQ=255)                                                                                                         tgCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTAcg                        >  2:833824/1‑139 (MQ=255)                                                                                                         tgCAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAACCAATGCGTACCGCGCCAAATTATTTAcg                        >  2:835654/1‑139 (MQ=255)                                                                                                           cAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTgc                                                                                          >  1:312273/1‑71 (MQ=255)                                                                                                           cAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTgc                                                                                          <  2:312273/71‑1 (MQ=255)                                                                                                           cAAAAATTACGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTAcgtc                      <  2:53778/139‑1 (MQ=255)                                                                                                                   aCGTAACCCGACAGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTc              <  2:202384/139‑1 (MQ=255)                                                                                                                               aGTGACATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGAtt  <  1:357024/139‑1 (MQ=255)                                                                                                                                   aCATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTg                                                                             >  2:632366/1‑60 (MQ=255)                                                                                                                                   aCATCCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTg                                                                             <  1:632366/60‑1 (MQ=255)                                                                                                                                       |                                                                                                                                   TAAAACAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGATT  >  NC_000913/2010611‑2010875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑