Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,044,891	G→A	intergenic (+267/‑47)	asnT → / → yeeJ	tRNA‑Asn/putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,044,891	0	G	A	100.0%	131.1 / NA	41	intergenic (+267/‑47)	asnT/yeeJ	tRNA‑Asn/putative adhesin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/21);  total (20/21)

ATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTGTGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGACAAATATTATGCGGGATGGGAATTAAACTACGCCG  >  NC_000913/2044778‑2045014                                                                                                                  |                                                                                                                            ataAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTa                                                                                                    <  2:677334/139‑1 (MQ=255)          gAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAGACAGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCa                                                                                           <  1:949452/139‑1 (MQ=255)           aGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAg                                                                                          <  1:992210/139‑1 (MQ=255)            gTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAgg                                                                                         <  1:582450/139‑1 (MQ=255)            cagaTTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAgg                                                                                         <  1:665420/136‑1 (MQ=255)                 tCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAgg                                                                                    >  1:258232/1‑139 (MQ=255)                        tctcCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCAt                                                                             <  2:924066/139‑1 (MQ=255)                          tcCCCTCTTGTAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAggg                                                                                        <  2:744205/126‑1 (MQ=255)                          tcCCCTCTTGTAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAggg                                                                                        >  1:744205/1‑126 (MQ=255)                          tcCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGCAAGGGAGCATTTATGTTAGCTCGTTCATGGAAGGTAAGCactg                                                                           >  2:665420/1‑136 (MQ=255)                          tcCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATgg                                                                           >  2:852945/1‑139 (MQ=255)                                    cAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACgaagaa                                                                 <  1:497719/139‑1 (MQ=255)                                       cTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAagaagag                                                              <  2:56306/139‑1 (MQ=255)                                         cacaCCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAagaagagaa                                                            >  1:857751/1‑139 (MQ=255)                                          acacCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAagaagagaag                                                           <  2:1051240/139‑1 (MQ=255)                                          acacCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAagaagagaag                                                           <  1:736719/139‑1 (MQ=255)                                              ccAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGga                                                       <  1:187460/139‑1 (MQ=255)                                                                 aGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGAc                                    <  1:281262/139‑1 (MQ=255)                                                                     attaATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGACAAat                                >  2:325444/1‑139 (MQ=255)                                                                      ttaATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCTGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGAc                                    >  2:232292/1‑134 (MQ=255)                                                                      ttaATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGAc                                    <  1:232292/134‑1 (MQ=255)                                                                             tCAATCATATTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGACAAATATTATGCg                        >  1:546265/1‑139 (MQ=255)                                                                                     atTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCa                                                                              <  1:719842/77‑1 (MQ=255)                                                                                     atTGCTATCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGAGAAGGTAAGCa                                                                              >  2:719842/1‑77 (MQ=255)                                                                                           aTCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGAc                                    >  1:879357/1‑113 (MQ=255)                                                                                           aTCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGAc                                    <  2:879357/113‑1 (MQ=255)                                                                                           aTCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGACAAATATTATg                          >  2:464765/1‑123 (MQ=255)                                                                                           aTCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGACAAATATTATg                          <  1:464765/123‑1 (MQ=255)                                                                                           aTCAGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGACAAATATTATGCGGGATGGGAATTaaa          >  2:770739/1‑139 (MQ=255)                                                                                              aGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAATTGGAGAAGAAATAAATGACCGACAAATATTATGCGGGATGGGAATTAAACTa       >  2:553919/1‑139 (MQ=255)                                                                                              aGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGACAAATATTATGCGGGATGGGAATTAAACTa       <  1:325444/139‑1 (MQ=255)                                                                                              aGTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGACAAATATTATGCGGGATGGGAATTAAACTa       >  2:682848/1‑139 (MQ=255)                                                                                               gTATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGACAAATATTATGCGGGATGGGAATTAAACTAc      >  1:519609/1‑139 (MQ=255)                                                                                                tATTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGACAAATATTATGCGGGATGGGAATTAAACTAcg     >  1:912556/1‑139 (MQ=255)                                                                                                 aTTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCAt                                                                             <  1:68878/66‑1 (MQ=255)                                                                                                 aTTTACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCAt                                                                             >  2:68878/1‑66 (MQ=255)                                                                                                   ttACATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGACAAATATTATGCGGGATGGGAATTAAACTAcgccg  >  2:435314/1‑139 (MQ=255)                                                                                                      cATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGAcc                                       >  2:286207/1‑99 (MQ=255)                                                                                                      cATTTTTTCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGAcc                                       <  1:286207/99‑1 (MQ=255)                                                                                                          ttttCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGACAAATATTATg                          >  2:50371/1‑108 (MQ=255)                                                                                                          ttttCGCTATGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGACAAATATTATg                          <  1:50371/108‑1 (MQ=255)                                                                                                                  |                                                                                                                            ATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATTGCTATCAGTATTTACATTTTTTCGCTGTGCTAGAAAGGGCGCATTTATGTTAGCTCGTTCAGGGAAGGTAAGCATGGCTACGAAGAAGAGAAGTGGAGAAGAAATAAATGACCGACAAATATTATGCGGGATGGGAATTAAACTACGCCG  >  NC_000913/2044778‑2045014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑