Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,059,093	G→A	L200L (CTG→TTG)	yeeO ←	putative multdrug exporter, MATE family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,059,093	0	G	A	100.0%	149.9 / NA	47	L200L (CTG→TTG)	yeeO	putative multdrug exporter, MATE family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/21);  total (26/21)

AGCACCCACAAAATTGCAACTGCGCCAATATAACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAGGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTC  >  NC_000913/2058957‑2059228                                                                                                                                         |                                                                                                                                        aGCACCCACAAAATTGCAACTGCGCCAATATAACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGc                                                                                                                                       <  2:383054/139‑1 (MQ=255)   cACCCACAAAATTGCAACTGCGCCAATATAACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTa                                                                                                                                     >  1:975509/1‑139 (MQ=255)      ccACAAAATTGCAACTGCGCCAATATAACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCg                                                                                                                                  <  1:845110/139‑1 (MQ=255)                    cTGCGCCAATATAACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGc                                                                                                                                       <  2:934140/120‑1 (MQ=255)                     tGCGCCAATATAACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAG‑AAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTa                                                                                                                  <  1:185171/139‑1 (MQ=255)                      gcgcCAATATAACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTa                                                                                                                  >  2:482708/1‑139 (MQ=255)                             tataACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGt                                                                                                           <  1:38500/139‑1 (MQ=255)                                aaCGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTAtt                                                                                                        <  2:649846/139‑1 (MQ=255)                                      aaaTGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGc                                                                                                  >  1:513989/1‑139 (MQ=255)                                       aaTGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCg                                                                                                                                  <  2:652797/106‑1 (MQ=255)                                       aaTGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCg                                                                                                                                  >  1:652797/1‑106 (MQ=255)                                         tGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGcacc                                                                                               >  1:852241/1‑139 (MQ=255)                                           gTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGcaccac                                                                                             >  2:697531/1‑139 (MQ=255)                                                   cAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGcc                                                                                     <  2:393457/139‑1 (MQ=255)                                                    aGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGGAGTCAAGAAATATGGGGATAATAATAATTATAAACATATGCCAGTTACGGTTAACAAATAGGGGTAGTTTTCTATTCCCTCCGCAACGAAGTGccc                                                                                    >  2:691350/1‑139 (MQ=255)                                                    aGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGccc                                                                                    <  2:44123/139‑1 (MQ=255)                                                           ccccGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTAcc                                                                             >  1:519040/1‑139 (MQ=255)                                                           ccccGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTAcc                                                                             >  1:892469/1‑139 (MQ=255)                                                                           tCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTAc                                                                                                                                    <  1:349318/68‑1 (MQ=255)                                                                           tCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTAc                                                                                                                                    >  2:349318/1‑68 (MQ=255)                                                                                gCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAgctgc                                                        >  1:964904/1‑139 (MQ=255)                                                                                 ccAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAgctgct                                                       <  2:16517/139‑1 (MQ=255)                                                                                     gagaAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTAc                                                                                                                                    <  1:1020357/58‑1 (MQ=255)                                                                                     gagaAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTAc                                                                                                                                    >  2:1020357/1‑58 (MQ=255)                                                                                          aaGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAAATg                                              >  2:1039356/1‑139 (MQ=255)                                                                                               cGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTAc                                         >  1:85619/1‑139 (MQ=255)                                                                                                  aaaTCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGt                                      >  2:661527/1‑139 (MQ=255)                                                                                                     tCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAg                                   <  2:892469/139‑1 (MQ=255)                                                                                                      cAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGc                                  <  2:666772/139‑1 (MQ=255)                                                                                                      cAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGc                                  <  2:551607/139‑1 (MQ=255)                                                                                                       aaTATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCt                                 <  1:661527/139‑1 (MQ=255)                                                                                                        atatGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTCTTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGc                                                              <  2:745098/109‑1 (MQ=255)                                                                                                        atatGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGc                                                              >  1:745098/1‑109 (MQ=255)                                                                                                             ccGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGAt                           <  2:513989/139‑1 (MQ=255)                                                                                                                cTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAg                        >  1:357302/1‑139 (MQ=255)                                                                                                                          tatTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAg              <  1:482708/139‑1 (MQ=255)                                                                                                                             tAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGc           >  1:218935/1‑139 (MQ=255)                                                                                                                               aGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCtt         >  2:718690/1‑139 (MQ=255)                                                                                                                               aGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGACAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCtt         >  1:455281/1‑139 (MQ=255)                                                                                                                                   tatTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCa               <  2:475458/129‑1 (MQ=255)                                                                                                                                   tatTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCa               >  1:475458/1‑129 (MQ=255)                                                                                                                                    atTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACt    >  2:866288/1‑139 (MQ=255)                                                                                                                                    atTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACt    >  1:241718/1‑139 (MQ=255)                                                                                                                                     ttCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCt                                                                                <  1:844323/62‑1 (MQ=255)                                                                                                                                     ttCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCt                                                                                >  2:844323/1‑62 (MQ=255)                                                                                                                                      tCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTc  >  1:513938/1‑139 (MQ=255)                                                                                                                                      tCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTc  >  2:316235/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                        AGCACCCACAAAATTGCAACTGCGCCAATATAACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAGGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTC  >  NC_000913/2058957‑2059228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑