Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,084,773 G→A G251G (GGC→GGT)  yeeE ← UPF0394 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,084,7730GA100.0% 156.7 / NA 49G251G (GGC→GGT) yeeEUPF0394 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/25);  total (24/25)

TGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGAT  >  NC_000913/2084637‑2084907
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tGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      <  2:503515/139‑1 (MQ=255)
tGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      >  1:61078/1‑139 (MQ=255)
     ccGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 <  2:912269/139‑1 (MQ=255)
     ccGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 <  1:895468/139‑1 (MQ=255)
        aaGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcccc                                                                                                                                    <  2:305797/133‑1 (MQ=255)
        aaGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcccc                                                                                                                                    >  1:305797/1‑133 (MQ=255)
         aGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAAt                                                                                                                             <  2:133368/139‑1 (MQ=255)
         aGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAAt                                                                                                                             <  1:440585/139‑1 (MQ=255)
          gCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATg                                                                                                                            <  1:724708/139‑1 (MQ=255)
               tCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTAttt                                                                                                                       <  1:137363/139‑1 (MQ=255)
                     caccacCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTc                                                                                                                 >  2:867041/1‑139 (MQ=255)
                        caccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 <  1:306377/120‑1 (MQ=255)
                        caccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 >  2:306377/1‑120 (MQ=255)
                          ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcg                                                                                                            >  1:232103/1‑139 (MQ=255)
                             aaCCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGa                                                                                                         >  2:501783/1‑139 (MQ=255)
                             aaCCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGa                                                                                                         >  1:896486/1‑139 (MQ=255)
                                  gCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGa                                                                                                    >  2:809270/1‑139 (MQ=255)
                                    tACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGaaa                                                                                                  >  2:820720/1‑139 (MQ=255)
                                    tACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGaaa                                                                                                  <  1:809270/139‑1 (MQ=255)
                                         aaTGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCa                                                                                             <  2:232103/139‑1 (MQ=255)
                                          aTGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAg                                                                                            <  1:1030106/139‑1 (MQ=255)
                                          aTGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAg                                                                                            <  1:501783/139‑1 (MQ=255)
                                                tttgtGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATAtt                                                                                      >  2:889647/1‑139 (MQ=255)
                                                tttgtGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATAtt                                                                                      >  1:304163/1‑139 (MQ=255)
                                                 ttgtGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTg                                                                                     >  1:842838/1‑139 (MQ=255)
                                                     gCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCg                                                                                 >  2:709911/1‑139 (MQ=255)
                                                         cAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTgg                                                                             <  2:286671/139‑1 (MQ=255)
                                                               cgcgAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGc                                                                                              >  1:4914/1‑116 (MQ=255)
                                                               cgcgAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGc                                                                                              <  2:4914/116‑1 (MQ=255)
                                                                gcgAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTg                                                                      <  1:867041/139‑1 (MQ=255)
                                                                    aCGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTc                                                                  <  2:304163/139‑1 (MQ=255)
                                                                                              tGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCt                                        <  1:860533/139‑1 (MQ=255)
                                                                                                          ccccACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGc                            <  1:349050/139‑1 (MQ=255)
                                                                                                          ccccACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGc                            <  2:484391/139‑1 (MQ=255)
                                                                                                             cACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAg                         >  1:914574/1‑139 (MQ=255)
                                                                                                                     tCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAg                 >  2:454711/1‑139 (MQ=255)
                                                                                                                     tCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCT‑ACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAgc                >  2:238173/1‑139 (MQ=255)
                                                                                                                               cAGGAAAACACCCCATTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTa                                                               <  1:791520/83‑1 (MQ=255)
                                                                                                                               cAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCaaa       >  1:143256/1‑139 (MQ=255)
                                                                                                                               cAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCaaa       >  2:23200/1‑139 (MQ=255)
                                                                                                                               cAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCaaa       <  1:454711/139‑1 (MQ=255)
                                                                                                                               cAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGAGAAGTGATTCCTa                                                               >  2:791520/1‑83 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGt                                                                       >  2:185482/1‑73 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGt                                                                       <  1:185482/73‑1 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTcc                                                                 >  1:740487/1‑79 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTcc                                                                 <  2:740487/79‑1 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAAcc     >  2:792186/1‑139 (MQ=255)
                                                                                                                                 ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAAcc     >  2:12087/1‑139 (MQ=255)
                                                                                                                                    aaaCACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGAt  <  1:148199/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
TGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGAT  >  NC_000913/2084637‑2084907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑