| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,152,876 | G→A | P85S (CCG→TCG) | yegL ← | VMA domain protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,152,876 | 0 | G | A | 96.3% | 158.2 / ‑1.7 | 54 | P85S (CCG→TCG) | yegL | VMA domain protein |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (31/21); total (32/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 | |||||||||||
GAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTA > NC_000913/2152748‑2153009 | gAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTa > 2:112776/1‑139 (MQ=255) gCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTa < 1:112776/139‑1 (MQ=255) ataataCGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAg > 1:608274/1‑139 (MQ=255) cGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGgttgt < 1:527745/139‑1 (MQ=255) aaaTACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTc < 1:885587/139‑1 (MQ=255) aaTACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTcc > 2:374126/1‑139 (MQ=255) tACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCcac > 1:764210/1‑139 (MQ=255) cATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATg > 1:304928/1‑139 (MQ=255) aTACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCCGCTCAAGGGGTGTTCCACATGCCCCGCGCCg > 2:693283/1‑139 (MQ=255) tCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACg > 1:223184/1‑139 (MQ=255) tCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAAt > 1:805653/1‑139 (MQ=255) tCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGCCGAAAAAAAATTAGCGGCGCTGGTAAACGGTTGTTCCACATGCACCGGGCCGAACGTCACAAt > 1:636842/1‑139 (MQ=255) cGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGc > 1:539158/1‑139 (MQ=255) ctctACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGt > 1:795211/1‑139 (MQ=255) ctctACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGt > 2:398372/1‑139 (MQ=255) tACCATATCCAGGGCTTTGGTAATGGCAGCACCCATTGGTGTATCGCCCTGGGCAAACAGGCTGGGCGAAAAAAAATTAACGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTcc > 2:639227/1‑139 (MQ=255) tatCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCActct > 1:642052/1‑139 (MQ=255) tCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGC‑GAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCtttt > 2:35922/1‑139 (MQ=255) ccAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTca > 2:289441/1‑117 (MQ=255) ccAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTca < 1:289441/117‑1 (MQ=255) aGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTa > 1:262147/1‑139 (MQ=255) ggCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAgc < 1:148204/69‑1 (MQ=255) ggCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAgc > 2:148204/1‑69 (MQ=255) ggCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGcc > 1:1014468/1‑105 (MQ=255) ggCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGcc < 2:1014468/105‑1 (MQ=255) ggCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTaaa > 2:842071/1‑139 (MQ=255) ggCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTaaa > 1:25600/1‑139 (MQ=255) cTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGc < 2:820029/139‑1 (MQ=255) ttGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGC‑GAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAg < 1:35922/139‑1 (MQ=255) tGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAg < 1:748075/139‑1 (MQ=255) gTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCg < 1:52229/139‑1 (MQ=255) ggCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCg < 2:45261/134‑1 (MQ=255) ggCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCg > 1:45261/1‑134 (MQ=255) ccGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCagc > 1:974826/1‑139 (MQ=255) cACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAg < 2:885577/127‑1 (MQ=255) cACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAg > 1:885577/1‑127 (MQ=255) ccATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAg < 2:533203/139‑1 (MQ=255) ccATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAg < 1:374126/139‑1 (MQ=255) cATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGt < 2:65367/139‑1 (MQ=255) cGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTtc > 1:728020/1‑130 (MQ=255) cGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTtc < 2:728020/130‑1 (MQ=255) cGCCCTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGaaa < 2:539158/139‑1 (MQ=255) cccTGGGCAAACAGGATGGGCGAAAAAAAATTAgcggcg < 2:205983/39‑1 (MQ=255) cccTGGGCAAACAGGATGGGCGAAAAAAAATTAgcggcg > 1:205983/1‑39 (MQ=255) cccTGGGCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACTTCACAATGCCGAGTTCCACTCTTTTTAGAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAgg > 1:637124/1‑139 (MQ=255) gggCAAACAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAAc > 2:317862/1‑139 (MQ=255) aCAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCAc < 2:886546/85‑1 (MQ=255) aCAGGATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCAc > 1:886546/1‑85 (MQ=255) gATGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCg < 2:642052/139‑1 (MQ=255) aTGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGcc < 1:430451/71‑1 (MQ=255) aTGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGcc > 2:430451/1‑71 (MQ=255) aTGGGCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGt > 1:606901/1‑139 (MQ=255) gggCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTa > 1:618559/1‑139 (MQ=255) gggCGAAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTa < 2:618559/139‑1 (MQ=255) | GAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTA > NC_000913/2152748‑2153009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |