Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,164,998	G→A	*241* (TAG→TAA)	baeR →	response regulator in two‑component regulatory system with BaeS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,164,998	0	G	A	100.0%	98.0 / NA	32	*241* (TAG→TAA)	baeR	response regulator in two‑component regulatory system with BaeS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/16);  total (16/16)

CGACCGCACCATCGACAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGG  >  NC_000913/2164866‑2165133                                                                                                                                     |                                                                                                                                        cgaccgCACCATCGACAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAg                                                                                                                                   <  1:593286/139‑1 (MQ=255)       cACCATCGACAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACat                                                                                                                             >  2:677150/1‑139 (MQ=255)                  gCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAg                                                                                                                  <  2:526945/139‑1 (MQ=255)                      caTTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCgg                                                                                                              >  1:817902/1‑139 (MQ=255)                           aaaaCCTGGGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTCTGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATg                                                                                                         <  2:646048/139‑1 (MQ=255)                                 tGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTc                                                                                                   >  1:218241/1‑139 (MQ=255)                                 tGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTc                                                                                                   >  2:362116/1‑139 (MQ=255)                                  gcgcCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCa                                                                                                  >  2:370893/1‑139 (MQ=255)                                   cgccgcAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAg                                                                                                 >  1:778526/1‑139 (MQ=255)                                              ggAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCgg                                                                                      <  1:881167/139‑1 (MQ=255)                                                    tctcGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACAt                                                                                <  1:984374/139‑1 (MQ=255)                                                     ctcGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCg                                                                                                                                 <  1:136440/89‑1 (MQ=255)                                                     ctcGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCg                                                                                                                                 >  2:136440/1‑89 (MQ=255)                                                     ctcGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACAtt                                                                               >  2:119231/1‑139 (MQ=255)                                                         aCGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATcc                                                                           <  2:473451/139‑1 (MQ=255)                                                             cGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACg                                                                       >  2:228537/1‑139 (MQ=255)                                                                     cATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCa                                                               <  2:999968/139‑1 (MQ=255)                                                                               cgcCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGAc                                                     >  1:630688/1‑139 (MQ=255)                                                                                gcCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGa                                                                                           >  2:962866/1‑100 (MQ=255)                                                                                gcCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGa                                                                                           <  1:962866/100‑1 (MQ=255)                                                                                gcCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACa                                                    >  2:854954/1‑139 (MQ=255)                                                                                gcCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACa                                                    >  1:557869/1‑139 (MQ=255)                                                                                gcCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACa                                                    >  1:1028023/1‑139 (MQ=255)                                                                                        tGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACAGTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTAtctct                                            <  2:447768/139‑1 (MQ=255)                                                                                             tCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGcc                                       >  2:839361/1‑139 (MQ=255)                                                                                              cGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGccc                                      >  1:789258/1‑139 (MQ=255)                                                                                                 tttccGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTgg                                   <  2:243694/136‑1 (MQ=255)                                                                                                     cGCTGGGAAGCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTggcagg                               <  1:200034/139‑1 (MQ=255)                                                                                                              gCCGACGCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGaaa                      <  2:817902/139‑1 (MQ=255)                                                                                                                    gCCTGCCGCATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCt                <  1:228537/139‑1 (MQ=255)                                                                                                                            cATCGTTTAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACttt        <  1:362116/139‑1 (MQ=255)                                                                                                                                  ttAATTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCgg  <  2:218241/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        CGACCGCACCATCGACAGCCACATTAAAAACCTGCGCCGCAAGCTGGAATCTCTCGACGCCGAACAGTCATTTATCCGCGCCGTTTATGGCGTCGGTTACCGCTGGGAAGCCGACGCCTGCCGCATCGTTTAGTTTTAGCGACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGG  >  NC_000913/2164866‑2165133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑