Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,175,450	G→A	A291V (GCG→GTG)	gatZ ←	D‑tagatose 1,6‑bisphosphate aldolase 2, subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,175,450	0	G	A	100.0%	211.3 / NA	66	A291V (GCG→GTG)	gatZ	D‑tagatose 1,6‑bisphosphate aldolase 2, subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/38);  total (28/38)

TAAAACCCGTACGATAATATTTTTTCCAGTATTGCGGTTCGTCGAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCGCCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGT  >  NC_000913/2175319‑2175574                                                                                                                                    |                                                                                                                             tAAAACCCGTACGATAATATTTTTTCCAGTATTGCGGTTCGTCGAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTcgcg                                                                                                                       <  2:824109/139‑1 (MQ=255)      cccGTACGATAATATTTTTTCCAGTATTGCGGTTCGTCGAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAg                                                                                                                  <  2:583825/139‑1 (MQ=255)        cGTACGATAATATTTTTTCCAGTATTGCGGTTCGTCGAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCa                                                                                                                <  2:540056/139‑1 (MQ=255)         gTACGATAATATTTTTTCCAGTATTGCGGTTCGTCGAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCAGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCaa                                                                                                               >  1:506402/1‑139 (MQ=255)          tACGATAATATTTTTTCCAGTATTGCGGTTCGTCGAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCAcc                                                                                                                            >  2:502989/1‑125 (MQ=255)          tACGATAATATTTTTTCCAGTATTGCGGTTCGTCGAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCAcc                                                                                                                            <  1:502989/125‑1 (MQ=255)          tACGATAATATTTTTTCCAGTATTGCGGTTCGTCGAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCaaa                                                                                                              >  2:930393/1‑139 (MQ=255)          tACGATAATATTTTTTCCAGTATTGCGGTTCGTCGAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCaaa                                                                                                              >  2:823387/1‑139 (MQ=255)             gATAATATTTTTTCCAGTATTGCGGTTCGTCGAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGt                                                                                                           <  1:491931/139‑1 (MQ=255)                        ttCCAGTATTGCGGTTCGTCGAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTCAAGCAAAGGTTAATGCGGGAc                                                                                                <  2:635650/139‑1 (MQ=255)                        ttCCAGTATTGCGGTTCGTCGAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGAc                                                                                                <  2:210161/139‑1 (MQ=255)                        ttCCAGTATTGCGGTTCGTCGAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGAc                                                                                                <  1:733990/139‑1 (MQ=255)                         tCCAGTATTGCGGTTCGTCGAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGAcc                                                                                               >  1:49521/1‑139 (MQ=255)                                       tcgtcgAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTg                                                                                 <  1:366819/139‑1 (MQ=255)                                        cgtcgAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACt                                                                                           <  1:245992/128‑1 (MQ=255)                                        cgtcgAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACt                                                                                           >  2:245992/1‑128 (MQ=255)                                          tcgAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGt                                                                                                           >  2:787037/1‑110 (MQ=255)                                          tcgAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGt                                                                                                           <  1:787037/110‑1 (MQ=255)                                          tcgAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGAc                                                                                                >  1:450336/1‑121 (MQ=255)                                          tcgAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGAc                                                                                                <  2:450336/121‑1 (MQ=255)                                             aGCATCACTTCTTCAATTACCACCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGt                                                                           >  2:385028/1‑139 (MQ=255)                                             aGCATCACCTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGAc                                                                                                <  2:112666/118‑1 (MQ=255)                                             aGCATCACCTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGAc                                                                                                >  1:112666/1‑118 (MQ=255)                                                    cttcttCATCTACCGCCTGGCAACCGCTGCGACTATCACCGCCGATAAGTTCCCGCTCCATTAGTTCCAGTGCAAATATCACCTCGCGTAAAGCCAAGGTTAATGCCGGATGGACTTTCCAGATTGCAAAGTGATcgcg                                                                    <  2:836076/139‑1 (MQ=255)                                                    cttcttCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATcgcg                                                                    >  2:856289/1‑139 (MQ=255)                                                         tcAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCaa                                                                                                               >  2:642080/1‑91 (MQ=255)                                                         tcAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCaa                                                                                                               <  1:642080/91‑1 (MQ=255)                                                           aaTTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCAGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAAt                                                             <  2:506402/139‑1 (MQ=255)                                                            aTTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAAtt                                                            >  2:797944/1‑139 (MQ=255)                                                               aCCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTccc                                                         >  1:1005639/1‑139 (MQ=255)                                                               aCCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTccc                                                         >  1:87781/1‑139 (MQ=255)                                                                  gCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCaa                                                                                                               <  1:217066/82‑1 (MQ=255)                                                                  gCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCaa                                                                                                               >  2:217066/1‑82 (MQ=255)                                                                     aGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACt                                                                >  2:1002208/1‑126 (MQ=255)                                                                     aGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACt                                                                <  1:1002208/126‑1 (MQ=255)                                                                           ccGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTcc                                             <  1:369707/139‑1 (MQ=255)                                                                                    ttttCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGt                                    <  2:106151/139‑1 (MQ=255)                                                                                    ttttCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGt                                    <  1:887070/139‑1 (MQ=255)                                                                                    ttttCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGt                                    <  1:797944/139‑1 (MQ=255)                                                                                    ttttCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGt                                    <  1:823387/139‑1 (MQ=255)                                                                                     tttCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTa                                   <  2:955325/139‑1 (MQ=255)                                                                                              cGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGa                          <  1:856289/139‑1 (MQ=255)                                                                                              cGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGa                          <  2:1005639/139‑1 (MQ=255)                                                                                              cGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGa                          <  2:87781/139‑1 (MQ=255)                                                                                              cGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGa                          <  2:150100/139‑1 (MQ=255)                                                                                                aTAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGc                                                                                                                 <  2:338346/50‑1 (MQ=255)                                                                                                aTAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGc                                                                                                                 >  1:338346/1‑50 (MQ=255)                                                                                                aTAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGTGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAAt                        >  2:80769/1‑139 (MQ=255)                                                                                                aTAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAAt                        >  1:492461/1‑139 (MQ=255)                                                                                                   aGTTCCTGCTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAAtgtg                     <  1:731824/139‑1 (MQ=255)                                                                                                           cTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAAtgtg                     <  2:695831/131‑1 (MQ=255)                                                                                                           cTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAAtgtg                     >  1:695831/1‑131 (MQ=255)                                                                                                           cTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATaa             >  2:914674/1‑139 (MQ=255)                                                                                                           cTCAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATaa             >  2:144908/1‑139 (MQ=255)                                                                                                             cAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATcgcg                                                                    >  1:854928/1‑82 (MQ=255)                                                                                                             cAATTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATcgcg                                                                    <  2:854928/82‑1 (MQ=255)                                                                                                               aTTTGTGCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCa         >  1:904759/1‑139 (MQ=255)                                                                                                                     gCCAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCggg   >  2:461304/1‑139 (MQ=255)                                                                                                                      ccAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGgt  <  2:423179/139‑1 (MQ=255)                                                                                                                      ccAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGgt  <  1:882377/139‑1 (MQ=255)                                                                                                                      ccAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGgt  <  2:492461/139‑1 (MQ=255)                                                                                                                      ccAGTGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGgt  <  1:756469/139‑1 (MQ=255)                                                                                                                          tGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCgg    >  1:819110/1‑133 (MQ=255)                                                                                                                          tGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCgg    <  2:819110/133‑1 (MQ=255)                                                                                                                          tGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGgt  >  1:586705/1‑135 (MQ=255)                                                                                                                          tGCAAATATCACCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGATTCATAAACCATTCGGgt  <  2:586705/135‑1 (MQ=255)                                                                                                                                    |                                                                                                                             TAAAACCCGTACGATAATATTTTTTCCAGTATTGCGGTTCGTCGAGCATCACTTCTTCAATTACCGCCAGGCAACCGCTGCGATTTTCAGGGGCGATAAGTTCCTGCTCAATTTGTGCCAGTGCAAATATCGCCTCGCGTAAAGCAAAGGTTAATGCGGGACCGACTTTCAATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTGGTAATCGGTAGAATGTGCTTCATAAACCATTCGGGT  >  NC_000913/2175319‑2175574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑