Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 200,214 C→T P763S (CCA→TCA)  bamA → BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913200,2140CT100.0% 306.4 / NA 93P763S (CCA→TCA) bamABamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/47);  total (46/47)

TCATCACCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATCG  >  NC_000913/200080‑200346
                                                                                                                                      |                                                                                                                                    
tcatcaCCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAg                                                                                                                                  <  1:206426/139‑1 (MQ=255)
  atcaCCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCa                                                                                                                                <  1:869895/139‑1 (MQ=255)
  atcaCCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCa                                                                                                                                <  1:175141/139‑1 (MQ=255)
    caCCCCGACGCGGTTTATTAGCGATATGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAat                                                                                                                              <  1:103577/139‑1 (MQ=255)
      ccccGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAatat                                                                                                                            <  1:163628/139‑1 (MQ=255)
                 tttATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTg                                                                                                                 >  2:243404/1‑139 (MQ=255)
                     ttaGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg                                                                                                             <  1:1028358/139‑1 (MQ=255)
                       aGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTa                                                                                                           <  2:434721/139‑1 (MQ=255)
                        gCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTAt                                                                                                          <  1:243404/139‑1 (MQ=255)
                         cGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATc                                                                                                         <  1:212581/139‑1 (MQ=255)
                            tAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCa                                                                                                      <  2:970362/139‑1 (MQ=255)
                            tAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCa                                                                                                      <  1:825920/139‑1 (MQ=255)
                             aaGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCAt                                                                                                     <  1:882104/139‑1 (MQ=255)
                             aaGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCAt                                                                                                     <  2:529947/139‑1 (MQ=255)
                             aaGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCAt                                                                                                     <  1:254760/139‑1 (MQ=255)
                                  tGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACaa                                                                                                <  2:693759/139‑1 (MQ=255)
                                  tGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACaa                                                                                                >  2:238016/1‑139 (MQ=255)
                                      aaCTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg                                                                                                             <  2:3053/122‑1 (MQ=255)
                                      aaCTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg                                                                                                             >  1:3053/1‑122 (MQ=255)
                                          cGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTc                                                                                        >  1:1048719/1‑139 (MQ=255)
                                          cGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTc                                                                                        >  1:907691/1‑139 (MQ=255)
                                            gTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg                                                                                                             <  2:969592/116‑1 (MQ=255)
                                            gTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg                                                                                                             >  1:969592/1‑116 (MQ=255)
                                             ttCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTcccc                                                                                     >  2:227180/1‑139 (MQ=255)
                                                 tACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTg                                                                                 >  2:285811/1‑139 (MQ=255)
                                                 tACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTg                                                                                 >  1:171543/1‑139 (MQ=255)
                                                      ccttcTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGcc                                                                            >  2:851622/1‑139 (MQ=255)
                                                          cttctGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTg                                                                        <  1:985123/139‑1 (MQ=255)
                                                                ggATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGa                                                                                            >  2:225574/1‑113 (MQ=255)
                                                                ggATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGa                                                                                            <  1:225574/113‑1 (MQ=255)
                                                                ggATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTtc                                                                  >  1:420475/1‑139 (MQ=255)
                                                                     tGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCa                                                                                                                                >  1:256868/1‑72 (MQ=255)
                                                                     tGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCa                                                                                                                                <  2:256868/72‑1 (MQ=255)
                                                                      gggTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGt                                                                                                                        >  2:202708/1‑79 (MQ=255)
                                                                      gggTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGt                                                                                                                        >  1:283807/1‑79 (MQ=255)
                                                                      gggTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGt                                                                                                                        <  1:202708/79‑1 (MQ=255)
                                                                      gggTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGt                                                                                                                        <  2:283807/79‑1 (MQ=255)
                                                                          aCCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGccc                                                        >  2:552586/1‑139 (MQ=255)
                                                                          aCCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGccc                                                        >  1:724727/1‑139 (MQ=255)
                                                                          aCCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGccc                                                        >  1:44067/1‑139 (MQ=255)
                                                                           ccGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCTGTATGTCTGCGGGTATCGc                                                                                                       <  2:793861/91‑1 (MQ=255)
                                                                           ccGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCTGTATGTCTGCGGGTATCGc                                                                                                       >  1:793861/1‑91 (MQ=255)
                                                                           ccGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg                                                                                                             <  1:569587/85‑1 (MQ=255)
                                                                           ccGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCggg                                                                                                             >  2:569587/1‑85 (MQ=255)
                                                                           ccGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCa                                                       >  1:799684/1‑139 (MQ=255)
                                                                           ccGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCa                                                       >  1:762400/1‑139 (MQ=255)
                                                                            cGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAg                                                      >  1:327420/1‑139 (MQ=255)
                                                                             gTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGc                                                     <  1:542541/139‑1 (MQ=255)
                                                                              tttGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCa                                                                                                      <  2:334101/89‑1 (MQ=255)
                                                                              tttGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCa                                                                                                      >  1:334101/1‑89 (MQ=255)
                                                                              tttGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTggg                                                                               >  1:203170/1‑112 (MQ=255)
                                                                              tttGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTggg                                                                               <  2:203170/112‑1 (MQ=255)
                                                                              tttGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGgtg                                                                     >  1:635868/1‑122 (MQ=255)
                                                                              tttGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGgtg                                                                     <  2:635868/122‑1 (MQ=255)
                                                                              tttGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGcc                                                    >  2:922638/1‑139 (MQ=255)
                                                                                tGGGATACAAACTGGGCTTCCAGCCAATACTCTGCATATACGGACGATAGTGATTCAAGCAATATCCGTAAGTCTGCGGATATCGCATTACAATGGATGTCCCCATTGGGGTCGTTGGTGTTCTCCTACGCCCAGCCgg                                                  >  2:609235/1‑138 (MQ=255)
                                                                                tGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTATGCCCAGCCGt                                                  >  1:851058/1‑139 (MQ=255)
                                                                                      aCAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCt                                                                                                                  >  2:110572/1‑69 (MQ=255)
                                                                                      aCAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCt                                                                                                                  <  1:110572/69‑1 (MQ=255)
                                                                                        aaaCTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAg                                          <  2:762400/139‑1 (MQ=255)
                                                                                         aaCTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGt                                         <  2:171543/139‑1 (MQ=255)
                                                                                             gggATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCAt                                                                                                     >  1:228074/1‑75 (MQ=255)
                                                                                             gggATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCAt                                                                                                     <  2:228074/75‑1 (MQ=255)
                                                                                              ggATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCg                                                                                                        >  1:920043/1‑71 (MQ=255)
                                                                                              ggATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCg                                                                                                        <  2:920043/71‑1 (MQ=255)
                                                                                              ggATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGgtgt                                                                    <  2:680201/107‑1 (MQ=255)
                                                                                              ggATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGgtgt                                                                    >  1:680201/1‑107 (MQ=255)
                                                                                                 ttCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGAt                                    >  1:447910/1‑136 (MQ=255)
                                                                                                 ttCCAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGAt                                    <  2:447910/136‑1 (MQ=255)
                                                                                                   ccAGCCAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGgaga                               <  1:238016/139‑1 (MQ=255)
                                                                                                        cAATATTCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAgg                          <  2:907691/139‑1 (MQ=255)
                                                                                                             ttCTGGATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTATGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGaa                     <  2:851058/139‑1 (MQ=255)
                                                                                                                 ggATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACaa                                                                                                >  2:404645/1‑60 (MQ=255)
                                                                                                                 ggATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACaa                                                                                                <  1:404645/60‑1 (MQ=255)
                                                                                                                 ggATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGt                 <  1:922638/139‑1 (MQ=255)
                                                                                                                 ggATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGt                 >  2:737415/1‑139 (MQ=255)
                                                                                                                  gATATCCGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGtt                >  1:548517/1‑139 (MQ=255)
                                                                                                                       ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACaa                                                                                                >  1:255099/1‑54 (MQ=255)
                                                                                                                       ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACaa                                                                                                <  2:255099/54‑1 (MQ=255)
                                                                                                                       ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGgtg                                                                     >  2:42751/1‑81 (MQ=255)
                                                                                                                       ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGgtg                                                                     <  1:42751/81‑1 (MQ=255)
                                                                                                                       ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGt                                         <  2:146779/109‑1 (MQ=255)
                                                                                                                       ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGt                                         >  1:146779/1‑109 (MQ=255)
                                                                                                                       ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGAt                                    >  1:336554/1‑114 (MQ=255)
                                                                                                                       ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGAt                                    <  2:336554/114‑1 (MQ=255)
                                                                                                                       ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGt           >  1:41753/1‑139 (MQ=255)
                                                                                                                       ccGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGt           >  2:38847/1‑139 (MQ=255)
                                                                                                                        cGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCa                                                                                                      <  1:1036563/47‑1 (MQ=255)
                                                                                                                        cGGACTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCa                                                                                                      >  2:1036563/1‑47 (MQ=255)
                                                                                                                            cTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGa                                                                                            >  2:900137/1‑53 (MQ=255)
                                                                                                                            cTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGa                                                                                            <  1:900137/53‑1 (MQ=255)
                                                                                                                            cTATAGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAAc      <  1:737415/139‑1 (MQ=255)
                                                                                                                                aGTGATTCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATCg  >  1:726926/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                    
TCATCACCCCGACGCCGTTTATTAGCGATAAGTATGCTAACTCGGTTCGTACTTCCTTCTTCTGGGATATGGGTACCGTTTGGGATACAAACTGGGATTCCAGCCAATATTCTGGATATCCGGACTATAGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATCG  >  NC_000913/200080‑200346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: