Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,182,061 G→A *322* (TAG→TAA
L241L (CTA→TTA) 
yegV →
yegW ←
putative kinase
putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,182,0610GA100.0% 110.9 / NA 36*322* (TAG→TAA
L241L (CTA→TTA) 
yegV
yegW
putative kinase
putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/21);  total (15/21)

CGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCA  >  NC_000913/2181926‑2182182
                                                                                                                                       |                                                                                                                         
cGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATc                                                                                                                        >  1:997506/1‑139 (MQ=255)
     tGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGc                                                                                                                   >  2:611465/1‑139 (MQ=255)
        ccTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGAc                                                                                                                <  1:513038/139‑1 (MQ=255)
              gTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGc                                                                                                          <  1:587794/139‑1 (MQ=255)
                cTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTa                                                                                                        <  2:792921/139‑1 (MQ=255)
                cTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTa                                                                                                        <  2:51364/139‑1 (MQ=255)
                   ccACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATg                                                                                                     >  1:889548/1‑139 (MQ=255)
                   ccACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATg                                                                                                     <  1:5891/139‑1 (MQ=255)
                        ggCGGATGCCGTATTACTCGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTa                                                                                                <  1:710079/139‑1 (MQ=255)
                                   tATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGcc                                                                                     >  2:969651/1‑139 (MQ=255)
                                    aTTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCg                                                                                    <  1:1033843/139‑1 (MQ=255)
                                    aTTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCg                                                                                    <  2:832887/139‑1 (MQ=255)
                                           ggCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGt                                                                             >  1:416944/1‑139 (MQ=255)
                                           ggCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGt                                                                             <  1:377422/139‑1 (MQ=255)
                                             cAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATg                                                                                                     <  1:1046240/113‑1 (MQ=255)
                                             cAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATg                                                                                                     >  2:1046240/1‑113 (MQ=255)
                                                tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTg                                                                              >  1:573971/1‑133 (MQ=255)
                                                tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTg                                                                              <  2:573971/133‑1 (MQ=255)
                                                  cAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCaa                                                                      >  2:700191/1‑139 (MQ=255)
                                                     tgccgtcgtGGGTTGTCGGGCAGCGGGGCGGTGATTGTGCGCCACCGCGCGAGGCATTCCTCCTCGCAAACAGATACGTATAACTCACTGCGACAGTGGCTAATGCTGTACTCAATATGCCGTCGCCGATGGTCAAgcg                                                                   <  2:120402/136‑1 (MQ=255)
                                                     tGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAgcg                                                                   >  2:896984/1‑139 (MQ=255)
                                                          tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCAct                                                              >  1:933859/1‑139 (MQ=255)
                                                              gggTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgct                                                          <  1:969651/139‑1 (MQ=255)
                                                                gTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTCGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgcttg                                                        <  1:611465/139‑1 (MQ=255)
                                                                             ggggCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCg                                           <  2:490893/139‑1 (MQ=255)
                                                                              gggCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTg                                                                              >  2:243732/1‑103 (MQ=255)
                                                                              gggCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTg                                                                              <  1:243732/103‑1 (MQ=255)
                                                                                         gtgCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTAt                               <  2:454858/139‑1 (MQ=255)
                                                                                           gcgcCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCt                             <  2:115624/139‑1 (MQ=255)
                                                                                              ccAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACa                                                                                                               <  1:469831/54‑1 (MQ=255)
                                                                                              ccAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACa                                                                                                               >  2:469831/1‑54 (MQ=255)
                                                                                                                     cGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTg                                   >  2:58367/1‑107 (MQ=255)
                                                                                                                     cGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTg                                   <  1:58367/107‑1 (MQ=255)
                                                                                                                     cGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggc   >  2:799282/1‑139 (MQ=255)
                                                                                                                     cGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggc   >  1:324766/1‑139 (MQ=255)
                                                                                                                      gCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggca  <  1:700191/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                         
CGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCA  >  NC_000913/2181926‑2182182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: