Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,218,207	C→T	A117A (GCG→GCA)	yehY ←	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,218,207	0	C	T	100.0%	191.7 / NA	59	A117A (GCG→GCA)	yehY	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/29);  total (30/29)

TGAGAGATCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGCGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAAT  >  NC_000913/2218075‑2218332                                                                                                                                     |                                                                                                                              tGAGAGATCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGca                                                                                                                         >  2:670138/1‑139 (MQ=255)  gagagaTCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcag                                                                                                                        >  1:369404/1‑139 (MQ=255)   agagaTCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACTAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagc                                                                                                                       >  2:457687/1‑139 (MQ=255)    gagaTCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACTAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagcc                                                                                                                      <  1:457687/139‑1 (MQ=255)    gagaTCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagcc                                                                                                                      <  2:369404/139‑1 (MQ=255)    gagaTCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagcc                                                                                                                      <  2:1040183/139‑1 (MQ=255)          gTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGccagccag                                                                                                                >  1:822719/1‑139 (MQ=255)                 gTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCAc                                                                                                         >  1:883020/1‑139 (MQ=255)                  tGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACt                                                                                                        >  2:147488/1‑139 (MQ=255)                  tGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACt                                                                                                        >  2:50904/1‑139 (MQ=255)                   gCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTg                                                                                                       >  1:61333/1‑139 (MQ=255)                     cGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGcc                                                                                                     <  1:424885/139‑1 (MQ=255)                               cAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTa                                                                                           <  1:670138/139‑1 (MQ=255)                               cAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTa                                                                                           <  1:535190/139‑1 (MQ=255)                                        cGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagcc                                                                                                                      >  1:165233/1‑103 (MQ=255)                                        cGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagcc                                                                                                                      <  2:165233/103‑1 (MQ=255)                                        cGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAgc                                                                                  >  1:583226/1‑139 (MQ=255)                                         ggAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAgcg                                                                                 <  2:61333/139‑1 (MQ=255)                                             taatGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACt                                                                             >  1:207167/1‑139 (MQ=255)                                                tGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGcc                                                                          >  2:519505/1‑139 (MQ=255)                                                     aTCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCAtttt                                                                     >  1:503709/1‑139 (MQ=255)                                                        tGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTgcg                                                                  >  1:181467/1‑139 (MQ=255)                                                             atGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGt                                                             >  1:415930/1‑139 (MQ=255)                                                             atGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGt                                                             >  1:946439/1‑139 (MQ=255)                                                               gCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTg                                                           >  1:699611/1‑139 (MQ=255)                                                                    aacCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGGCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTcg                                                      >  2:670195/1‑139 (MQ=255)                                                                    aacCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTcg                                                      >  1:493194/1‑139 (MQ=255)                                                                    aacCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTcg                                                      >  1:218226/1‑139 (MQ=255)                                                                      ccAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTcgcc                                                    <  2:219024/139‑1 (MQ=255)                                                                        aGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCCGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTcgccgc                                                  <  1:670195/139‑1 (MQ=255)                                                                         gcgcCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAgcg                                                                                 <  2:699573/107‑1 (MQ=255)                                                                         gcgcCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAgcg                                                                                 >  1:699573/1‑107 (MQ=255)                                                                         gcgcCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCgccgcc                                                 >  2:71182/1‑139 (MQ=255)                                                                          cgcCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCt                                                >  2:711088/1‑139 (MQ=255)                                                                                aGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagcc                                                                                                                      <  1:843071/63‑1 (MQ=255)                                                                                aGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagcc                                                                                                                      >  2:843071/1‑63 (MQ=255)                                                                                   ggATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTc                                       <  2:822719/139‑1 (MQ=255)                                                                                   ggATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTc                                       <  2:218226/139‑1 (MQ=255)                                                                                      tGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTcccc                                    <  2:259060/139‑1 (MQ=255)                                                                                         gTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCcaca                                 <  2:699611/139‑1 (MQ=255)                                                                                          tGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCacac                                >  2:41924/1‑139 (MQ=255)                                                                                          tGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCacac                                >  1:492213/1‑139 (MQ=255)                                                                                                   ggcggATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAgccagcca                                                                                                                 >  2:355493/1‑49 (MQ=255)                                                                                                   ggcggATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAgccagcca                                                                                                                 <  1:355493/49‑1 (MQ=255)                                                                                                   ggcggATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAg                                          <  2:409332/120‑1 (MQ=255)                                                                                                   ggcggATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAg                                          >  1:409332/1‑120 (MQ=255)                                                                                                            cATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGaa              <  2:503709/139‑1 (MQ=255)                                                                                                            cATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGaa              <  1:77044/139‑1 (MQ=255)                                                                                                            cATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGaa              <  1:50904/139‑1 (MQ=255)                                                                                                            cATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGaa              <  1:14706/139‑1 (MQ=255)                                                                                                             aTCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAAt             <  2:492213/139‑1 (MQ=255)                                                                                                              tCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAAtt            <  2:883020/139‑1 (MQ=255)                                                                                                                     aGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTcgccgc                                                  >  2:754940/1‑94 (MQ=255)                                                                                                                     aGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTcgccgc                                                  <  1:754940/94‑1 (MQ=255)                                                                                                                      ggCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGa    <  2:493194/139‑1 (MQ=255)                                                                                                                        ccAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAAt  <  1:89130/139‑1 (MQ=255)                                                                                                                        ccAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAAt  <  1:41924/139‑1 (MQ=255)                                                                                                                                 ccAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTc                                       >  1:832301/1‑93 (MQ=255)                                                                                                                                 ccAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTc                                       <  2:832301/93‑1 (MQ=255)                                                                                                                                     |                                                                                                                              TGAGAGATCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGCGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAAT  >  NC_000913/2218075‑2218332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑