Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,225,044	C→T	*196* (TAG→TAA)	yohC ←	Yip1 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,225,044	0	C	T	100.0%	150.8 / NA	47	*196* (TAG→TAA)	yohC	Yip1 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/31);  total (16/31)

CCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGG  >  NC_000913/2224915‑2225178                                                                                                                                  |                                                                                                                                       ccgGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACaaa                                                                                                                               >  1:331074/1‑139 (MQ=255)        aaGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAACGAGAATCGTGAGTAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAAc                                                                                                                        <  2:312761/139‑1 (MQ=255)           gATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGt                                                                                                                     >  1:947379/1‑139 (MQ=255)                aCTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGccc                                                                                                                <  2:331074/139‑1 (MQ=255)                aCTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGccc                                                                                                                <  1:211694/139‑1 (MQ=255)                aCTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGccc                                                                                                                <  1:718604/139‑1 (MQ=255)                 cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGcccc                                                                                                               <  2:274030/139‑1 (MQ=255)                  tGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                              <  1:135226/139‑1 (MQ=255)                  tGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                              <  1:309768/139‑1 (MQ=255)                  tGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                              <  2:22182/139‑1 (MQ=255)                  tGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                              <  2:179897/139‑1 (MQ=255)                  tGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                              <  2:118317/139‑1 (MQ=255)                  tGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATACAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCa                                                                                                              <  2:743761/139‑1 (MQ=255)                        tAGTGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                        <  2:543304/139‑1 (MQ=255)                        tAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                        <  1:88889/139‑1 (MQ=255)                                           aGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGagcagc                                                                                     >  2:1009838/1‑139 (MQ=255)                                             cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                        >  2:39218/1‑118 (MQ=255)                                             cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                        <  1:39218/118‑1 (MQ=255)                                             cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCAc                                                                                   >  1:406564/1‑139 (MQ=255)                                                aaaGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTc                                                                                <  1:326291/139‑1 (MQ=255)                                                        aTCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTc                                                                                <  1:782290/131‑1 (MQ=255)                                                        aTCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTc                                                                                >  2:782290/1‑131 (MQ=255)                                                                      aTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGcc                                                          >  2:821738/1‑139 (MQ=255)                                                                        cGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAg                                                        <  2:382370/139‑1 (MQ=255)                                                                         gTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAgg                                                                                              <  2:101581/100‑1 (MQ=255)                                                                         gTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAgg                                                                                              >  1:101581/1‑100 (MQ=255)                                                                          tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAg                                                        >  2:428425/1‑137 (MQ=255)                                                                          tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAg                                                        <  1:428425/137‑1 (MQ=255)                                                                          tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGca                                                      >  1:13480/1‑138 (MQ=255)                                                                          tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCg                                                      >  2:227347/1‑139 (MQ=255)                                                                          tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCg                                                      >  1:197364/1‑139 (MQ=255)                                                                          tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCg                                                      <  2:406564/139‑1 (MQ=255)                                                                          tGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCg                                                      >  1:725101/1‑139 (MQ=255)                                                                           gAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGt                                                     <  2:457952/139‑1 (MQ=255)                                                                                     tgcATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTagag                                           <  2:725101/139‑1 (MQ=255)                                                                                           gCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCTGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTa                                     <  2:474352/139‑1 (MQ=255)                                                                                                    tACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTcc                            >  1:15067/1‑139 (MQ=255)                                                                                                    tACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTcc                            <  2:499948/139‑1 (MQ=255)                                                                                                    tACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCATCGAACTAGAGAAGCTAAGGCCTTcc                            <  2:13480/139‑1 (MQ=255)                                                                                                     aCGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCct                           <  2:947379/139‑1 (MQ=255)                                                                                                     aCGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCct                           <  2:197364/139‑1 (MQ=255)                                                                                                           gCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCg                                                                                           <  1:241148/69‑1 (MQ=255)                                                                                                           gCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCg                                                                                           >  2:241148/1‑69 (MQ=255)                                                                                                                 cAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGatat               >  1:8211/1‑139 (MQ=255)                                                                                                                           gAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATAGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCt     <  2:908026/139‑1 (MQ=255)                                                                                                                             aCTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCGCGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCg   <  2:242921/139‑1 (MQ=255)                                                                                                                              cTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCgg  <  2:8211/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       CCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGG  >  NC_000913/2224915‑2225178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑