Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,244,245	G→A	A112A (GCG→GCA)	yeiG →	S‑formylglutathione hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,244,245	0	G	A	100.0%	161.5 / NA	50	A112A (GCG→GCA)	yeiG	S‑formylglutathione hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/23);  total (27/23)

GGCGGAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTG  >  NC_000913/2244116‑2244377                                                                                                                                  |                                                                                                                                     ggcggAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGTGAAAAAGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAc                                                                                                                             <  1:546509/139‑1 (MQ=255) ggcggAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAc                                                                                                                             <  1:567765/139‑1 (MQ=255)  gcggAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAcc                                                                                                                            <  1:209063/139‑1 (MQ=255)  gcggAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAcc                                                                                                                            <  1:639688/139‑1 (MQ=255)      aaCTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt                                                                                                                        <  2:949687/139‑1 (MQ=255)                         gATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGAt                                                                                                     >  2:735553/1‑139 (MQ=255)                          aTGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATg                                                                                                    >  1:273879/1‑139 (MQ=255)                            gCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAcc                                                                                                                            <  2:688111/113‑1 (MQ=255)                            gCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAcc                                                                                                                            >  1:688111/1‑113 (MQ=255)                            gCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGaa                                                                                                  >  1:952369/1‑139 (MQ=255)                                 acacCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTAcc                                                                                             >  1:969517/1‑139 (MQ=255)                                     cAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGgcg                                                                                         >  2:422386/1‑139 (MQ=255)                                       gCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCt                                                                                       >  2:659543/1‑139 (MQ=255)                                           gcgcgGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGtt                                                                                   <  1:903552/139‑1 (MQ=255)                                           gcgcgGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGtt                                                                                   >  1:418318/1‑139 (MQ=255)                                                 cgAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCg                                                                             <  1:157727/139‑1 (MQ=255)                                                            ccAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTc                                                                              <  1:722812/127‑1 (MQ=255)                                                            ccAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTc                                                                              >  2:722812/1‑127 (MQ=255)                                                                cgacgaTGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGc                                                              <  2:394366/139‑1 (MQ=255)                                                                     aTGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTgcg                                                                                                         <  1:51732/91‑1 (MQ=255)                                                                     aTGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTgcg                                                                                                         >  2:51732/1‑91 (MQ=255)                                                                        gCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTg                                                      <  2:418318/139‑1 (MQ=255)                                                                                  aGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGTCAACGCATTACCGGAt                                                                                                                        <  2:16391/63‑1 (MQ=255)                                                                                  aGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt                                                                                                                        >  1:16391/1‑63 (MQ=255)                                                                                  aGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGc                                            >  1:271318/1‑139 (MQ=255)                                                                                  aGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGc                                            >  2:124413/1‑139 (MQ=255)                                                                                     ccAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGt                                         <  1:971419/139‑1 (MQ=255)                                                                                     ccAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATAACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGt                                         <  1:699339/139‑1 (MQ=255)                                                                                         ggCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGATTTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTgcg                                                    >  2:668026/1‑124 (MQ=255)                                                                                         ggCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTgcg                                                    <  1:668026/124‑1 (MQ=255)                                                                                         ggCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACt                                     >  2:728288/1‑139 (MQ=255)                                                                                                          tAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTg                                                      <  2:383511/105‑1 (MQ=255)                                                                                                          tAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTg                                                      >  1:383511/1‑105 (MQ=255)                                                                                                          tAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGt                    >  1:514032/1‑139 (MQ=255)                                                                                                          tAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGt                    >  1:238393/1‑139 (MQ=255)                                                                                                          tAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGt                    >  1:415847/1‑139 (MQ=255)                                                                                                           aaTGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGAcc                                                          <  2:353217/100‑1 (MQ=255)                                                                                                           aaTGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGAcc                                                          >  1:353217/1‑100 (MQ=255)                                                                                                             tGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGt                    >  1:673273/1‑136 (MQ=255)                                                                                                             tGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGt                    <  2:673273/136‑1 (MQ=255)                                                                                                             tGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTgcg                 <  2:415847/139‑1 (MQ=255)                                                                                                                 aCGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTgcg                                                                                                         >  2:425611/1‑47 (MQ=255)                                                                                                                 aCGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTgcg                                                                                                         <  1:425611/47‑1 (MQ=255)                                                                                                                 aCGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGTATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGa             >  1:711406/1‑139 (MQ=255)                                                                                                                  cGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATg                                >  2:996631/1‑119 (MQ=255)                                                                                                                  cGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATg                                <  1:996631/119‑1 (MQ=255)                                                                                                                  cGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGAt            >  2:861800/1‑139 (MQ=255)                                                                                                                        cgccgTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACg                      <  2:729643/123‑1 (MQ=255)                                                                                                                        cgccgTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACg                      >  1:729643/1‑123 (MQ=255)                                                                                                                            gTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTg  >  2:514818/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                     GGCGGAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTG  >  NC_000913/2244116‑2244377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑