Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,261,864	T→C	S168G (AGT→GGT)	fruK ←	fructose‑1‑phosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,261,864	0	T	C	100.0%	147.1 / NA	41	S168G (AGT→GGT)	fruK	fructose‑1‑phosphate kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/27);  total (14/27)

GTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAG  >  NC_000913/2261729‑2261985                                                                                                                                        |                                                                                                                          gTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATc                                                                                                                        >  1:768003/1‑139 (MQ=255)  tAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCa                                                                                                                       >  1:453961/1‑139 (MQ=255)     cgcATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAg                                                                                                                    >  1:878501/1‑139 (MQ=255)       cATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGAt                                                                                                                  <  1:214619/139‑1 (MQ=255)             cAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCa                                                                                                            <  2:154398/139‑1 (MQ=255)               gCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAg                                                                                                          <  2:210033/139‑1 (MQ=255)                  tCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGac                                                                                                       <  2:1048355/139‑1 (MQ=255)                   cAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACAAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGaca                                                                                                      >  1:378991/1‑139 (MQ=255)                     aTCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACt                                                                                                    <  1:164372/139‑1 (MQ=255)                       cacaTCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGa                                                                                                  <  1:691107/139‑1 (MQ=255)                       cacaTCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGa                                                                                                  <  2:240705/139‑1 (MQ=255)                          aTCTTTCATTTCAGGC‑GTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTAc                                                                                              >  2:947811/1‑139 (MQ=255)                               tcatttcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACAAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAg                                                                                          <  2:378991/139‑1 (MQ=255)                                catttcaGGC‑GTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGc                                                                                        <  1:947811/139‑1 (MQ=255)                                       ggCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATaa                                                                                                                <  2:509659/109‑1 (MQ=255)                                       ggCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATaa                                                                                                                >  1:509659/1‑109 (MQ=255)                                        gCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCAt                                                                                 >  2:398547/1‑139 (MQ=255)                                         cAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATc                                                                                <  2:903110/139‑1 (MQ=255)                                         cAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATc                                                                                <  2:768003/139‑1 (MQ=255)                                          aGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATcc                                                                               <  2:1043034/139‑1 (MQ=255)                                           gTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATc                                                                                                                        >  2:560249/1‑97 (MQ=255)                                           gTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATc                                                                                                                        <  1:560249/97‑1 (MQ=255)                                                    cTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTCCGCAGGCGAGTCATCCAGTCGGTGaa                                                                     <  2:367209/139‑1 (MQ=255)                                                    cTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGaa                                                                     <  2:976073/139‑1 (MQ=255)                                                      gCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACg                                                                   >  1:411467/1‑139 (MQ=255)                                                                      cgcgGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGcc                                                   <  2:922494/139‑1 (MQ=255)                                                                          gcggTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGAc                                               <  2:878501/139‑1 (MQ=255)                                                                                  gTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCt                                       <  2:942682/139‑1 (MQ=255)                                                                                  gTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCACCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCt                                       <  2:322640/139‑1 (MQ=255)                                                                                      cACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCg                                   <  2:449999/139‑1 (MQ=255)                                                                                            ccACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGaca                             >  2:910261/1‑139 (MQ=255)                                                                                                  tGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGAcc                       <  2:453961/139‑1 (MQ=255)                                                                                                     cGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCata                    >  2:288686/1‑139 (MQ=255)                                                                                                          tCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGaa               <  1:183997/139‑1 (MQ=255)                                                                                                                   ctactaACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAg      >  2:779302/1‑139 (MQ=255)                                                                                                                   ctactaACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAg      <  2:411467/139‑1 (MQ=255)                                                                                                                       taACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAg  <  1:67138/139‑1 (MQ=255)                                                                                                                          cGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCata                    <  2:246349/118‑1 (MQ=255)                                                                                                                          cGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCata                    >  1:246349/1‑118 (MQ=255)                                                                                                                              tCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGa                         >  1:956876/1‑109 (MQ=255)                                                                                                                              tCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGa                         <  2:956876/109‑1 (MQ=255)                                                                                                                                        |                                                                                                                          GTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAG  >  NC_000913/2261729‑2261985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑