Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,621,307	A→G	E37E (GAA→GAG)	purM →	phosphoribosylaminoimidazole synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,621,307	0	A	G	100.0%	175.0 / NA	48	E37E (GAA→GAG)	purM	phosphoribosylaminoimidazole synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/20);  total (28/20)

CAAGTAACGCGTGGGGACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGAC  >  NC_000913/2621172‑2621439                                                                                                                                        |                                                                                                                                     cAAGTAACGCGTGGGGACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTg                                                                                                                                   <  2:747808/139‑1 (MQ=255)      aaCGCGTGGGGACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATggg                                                                                                                              <  1:846160/139‑1 (MQ=255)            tGGGGACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCt                                                                                                                        >  1:417966/1‑139 (MQ=255)              gggACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTgg                                                                                                                      >  1:602621/1‑139 (MQ=255)                             aCCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGt                                                                                                                          >  2:253149/1‑120 (MQ=255)                             aCCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGt                                                                                                                          <  1:253149/120‑1 (MQ=255)                                 aTAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCtgtg                                                                                                   >  2:468311/1‑139 (MQ=255)                                 aTAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCtgtg                                                                                                   >  2:41021/1‑139 (MQ=255)                                         tctctTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCt                                                                                                                <  2:556889/118‑1 (MQ=255)                                         tctctTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCt                                                                                                                >  1:556889/1‑118 (MQ=255)                                                 cTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCaaaaa                                                                                   >  1:476919/1‑139 (MQ=255)                                                     aaaGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATc                                                                               >  2:298035/1‑139 (MQ=255)                                                       aGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGt                                                                             >  1:1017924/1‑139 (MQ=255)                                                         aTGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGa                                                                           >  2:342888/1‑139 (MQ=255)                                                              ggTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGt                                                                                                           >  2:490605/1‑102 (MQ=255)                                                              ggTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGt                                                                                                           <  1:490605/102‑1 (MQ=255)                                                              ggTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCg                                                                      >  2:807254/1‑139 (MQ=255)                                                                   tGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTg                                                                 >  1:1012947/1‑139 (MQ=255)                                                                   tGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGACGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTg                                                                 >  2:667159/1‑139 (MQ=255)                                                                         tGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCt                                                           <  1:667159/139‑1 (MQ=255)                                                                            cgcgGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                        <  1:4544/91‑1 (MQ=255)                                                                            cgcgGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                        >  2:4544/1‑91 (MQ=255)                                                                            cgcgGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGc                                                        <  2:1012947/139‑1 (MQ=255)                                                                                ggTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTg                                                    >  2:168561/1‑139 (MQ=255)                                                                                ggTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTg                                                    >  1:1008215/1‑139 (MQ=255)                                                                                       ctctGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGt                                             <  2:1017924/139‑1 (MQ=255)                                                                                          tGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAgg                                          >  1:455910/1‑139 (MQ=255)                                                                                                gaagaaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCaa                                    >  2:941432/1‑139 (MQ=255)                                                                                                 aagaaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAg                                   >  2:155363/1‑139 (MQ=255)                                                                                                 aagaaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAg                                   <  2:417966/139‑1 (MQ=255)                                                                                                   gaaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCt                                 <  1:41021/139‑1 (MQ=255)                                                                                                      tCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCg                              >  1:665914/1‑139 (MQ=255)                                                                                                      tCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCg                              >  2:138217/1‑139 (MQ=255)                                                                                                      tCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCg                              >  1:158199/1‑139 (MQ=255)                                                                                                              gTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTCTGCATTGCCGTAAAAAGATCGTGAACCCGTGCTGGTTTCTTGCACTGACGGCGTAGGTTCTTAGTTGCGTCTGGCaa                      <  2:301770/139‑1 (MQ=255)                                                                                                               tAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAAt                     <  1:1005605/139‑1 (MQ=255)                                                                                                               tAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAAt                     <  2:988004/139‑1 (MQ=255)                                                                                                               tAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAAt                     <  1:298035/139‑1 (MQ=255)                                                                                                               tAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAAt                     >  1:297764/1‑139 (MQ=255)                                                                                                                       aaaaCGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTaa             <  1:138217/139‑1 (MQ=255)                                                                                                                         aaCGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCACTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTaaaa           >  2:375032/1‑139 (MQ=255)                                                                                                                            gcgTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGt        <  1:834024/139‑1 (MQ=255)                                                                                                                            gcgTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGt        >  1:818614/1‑139 (MQ=255)                                                                                                                            gcgTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGt        >  1:1045564/1‑139 (MQ=255)                                                                                                                              gtcgtcCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCa      >  1:951132/1‑139 (MQ=255)                                                                                                                                 gtcCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGa   <  2:962981/139‑1 (MQ=255)                                                                                                                                  tcCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGac  <  2:818614/139‑1 (MQ=255)                                                                                                                                  tcCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCACTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGac  <  1:375032/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     CAAGTAACGCGTGGGGACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGAC  >  NC_000913/2621172‑2621439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑