Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,721,193	C→T	A414V (GCA→GTA)	pka →	protein lysine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,721,193	0	C	T	100.0%	130.5 / NA	41	A414V (GCA→GTA)	pka	protein lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/22);  total (19/22)

ATTCGCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCG  >  NC_000913/2721064‑2721314                                                                                                                                  |                                                                                                                          attcGCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGAAGttt                                                                                                                  >  2:17373/1‑139 (MQ=255)   tcGCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTAt                                                                                                                >  2:899586/1‑139 (MQ=255)      cccAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAAAGTGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCa                                                                                                             >  2:752584/1‑139 (MQ=255)         aGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCg                                                                                                          >  1:938060/1‑139 (MQ=255)              cgcTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGcc                                                                                                     <  1:924568/139‑1 (MQ=255)                  gctCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCACAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGc                                                                                                 <  2:167029/139‑1 (MQ=255)                       cGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCg                                                                                            >  2:224817/1‑139 (MQ=255)                         cAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGAc                                                                                          <  1:224817/139‑1 (MQ=255)                                aaGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGt                                                                                   <  1:899586/139‑1 (MQ=255)                                       cAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCgg                                                                            <  2:902715/139‑1 (MQ=255)                                         aGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCggaa                                                                          >  2:741757/1‑139 (MQ=255)                                                   gAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCa                                                                <  1:113598/139‑1 (MQ=255)                                                    aaGCGGTAAAGCATCATTCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTGCTCGCAAGAGGTACGACGTTTATTCAGCGATGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCat                                                               <  1:650128/139‑2 (MQ=255)                                                    aaGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCAc                                                               >  1:257654/1‑139 (MQ=255)                                                     aGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGa                                                                                           <  1:527392/110‑1 (MQ=255)                                                     aGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGa                                                                                           >  2:527392/1‑110 (MQ=255)                                                          tAAAGCATCATCCCCGCAGC‑AATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCtttt                                                        <  1:220594/139‑1 (MQ=255)                                                             aGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTAt                                                      <  2:938060/139‑1 (MQ=255)                                                             aGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTAt                                                      <  2:275728/139‑1 (MQ=255)                                                               catcatCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGc                                                    <  1:1019281/139‑1 (MQ=255)                                                                   atcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTa                                                                                       <  1:926388/100‑1 (MQ=255)                                                                   atcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTa                                                                                       >  2:926388/1‑100 (MQ=255)                                                                   atcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCatat                                                <  1:741757/139‑1 (MQ=255)                                                                    tcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGTACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATAtg                                               >  2:162104/1‑139 (MQ=255)                                                                    tcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCg                                                                                    >  2:180117/1‑102 (MQ=255)                                                                    tcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCg                                                                                    <  1:180117/102‑1 (MQ=255)                                                                    tcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTa                                                       <  1:736097/131‑1 (MQ=255)                                                                    tcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTa                                                       >  2:736097/1‑131 (MQ=255)                                                                                atatGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGTAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCgg                                   >  2:876833/1‑139 (MQ=255)                                                                                  atGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCg                                 <  1:803731/139‑1 (MQ=255)                                                                                           tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCt                                                           <  2:19176/104‑1 (MQ=255)                                                                                           tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCt                                                           >  1:19176/1‑104 (MQ=255)                                                                                                aCGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTg                                                             <  2:960304/97‑1 (MQ=255)                                                                                                aCGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTg                                                             >  1:960304/1‑97 (MQ=255)                                                                                                 cGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACt                  >  2:39195/1‑139 (MQ=255)                                                                                                     cTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCatat                                                >  1:396711/1‑105 (MQ=255)                                                                                                     cTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCatat                                                <  2:396711/105‑1 (MQ=255)                                                                                                                aGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGc   >  2:696548/1‑139 (MQ=255)                                                                                                                 gCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCg  <  1:936121/139‑1 (MQ=255)                                                                                                                          gCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGAc                                                                                          <  1:927427/42‑1 (MQ=255)                                                                                                                          gCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGAc                                                                                          >  2:927427/1‑42 (MQ=255)                                                                                                                                  |                                                                                                                          ATTCGCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCG  >  NC_000913/2721064‑2721314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑