Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,724,448	C→T	H107H (CAC→CAT)  *433* (TAG→TAA)	yfiM → kgtP ←	putative lipoprotein alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,724,448	0	C	T	100.0%	138.8 / NA	44	H107H (CAC→CAT)  *433* (TAG→TAA)	yfiM kgtP	putative lipoprotein alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/25);  total (19/25)

TGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGT  >  NC_000913/2724312‑2724575                                                                                                                                         |                                                                                                                                tGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTa                                                                                                                               >  1:596215/1‑139 (MQ=255)     ctctGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGa                                                                                                                           <  1:30331/139‑1 (MQ=255)        tGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGc                                                                                                                        <  1:946786/139‑1 (MQ=255)        tGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGc                                                                                                                        <  1:731404/139‑1 (MQ=255)        tGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGc                                                                                                                        <  2:850590/139‑1 (MQ=255)              tttGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATcccc                                                                                                                  <  1:463775/139‑1 (MQ=255)                    ggCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCcccttccct                                                                                                            <  1:749585/139‑1 (MQ=255)                       gTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTg                                                                                                         <  1:223937/139‑1 (MQ=255)                        tCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGc                                                                                                        >  1:527028/1‑139 (MQ=255)                                    tGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCA‑CGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCaa                                                                                           >  1:198015/1‑139 (MQ=255)                                                 ccGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGaaa                                                                               >  1:448341/1‑139 (MQ=255)                                                      gggAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGcca                                                                          <  2:81164/139‑1 (MQ=255)                                                                      ggaAGGATTTGGCCTGGGATGTCGCCGGTGTAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGt                                                          <  2:659215/139‑1 (MQ=255)                                                                      ggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGt                                                          <  1:187705/139‑1 (MQ=255)                                                                      ggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGt                                                          <  2:441330/139‑1 (MQ=255)                                                                      ggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGt                                                          <  2:527028/139‑1 (MQ=255)                                                                            aTTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATa                                                    >  1:791758/1‑139 (MQ=255)                                                                              ttGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACAt                                                     <  1:603161/136‑1 (MQ=255)                                                                              ttGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACAt                                                     >  2:603161/1‑136 (MQ=255)                                                                                 gCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGaaa                                                                               <  1:361118/107‑1 (MQ=255)                                                                                 gCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGaaa                                                                               >  2:361118/1‑107 (MQ=255)                                                                                      ggATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATAc                                                   >  1:477013/1‑130 (MQ=255)                                                                                      ggATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATAc                                                   <  2:477013/130‑1 (MQ=255)                                                                                      ggATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGaagaag                                          >  1:1055947/1‑139 (MQ=255)                                                                                        aTGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGc                                        <  2:315849/139‑1 (MQ=255)                                                                                         tGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCt                                       <  2:448341/139‑1 (MQ=255)                                                                                                ggTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCCACATTAAACACGCATCCCCTTCCCTTTGCGATGCAGGATGAAAGCAACCAGAAACGCCACCACAGCCCTCACGGTCACCTACCAGAAGACGACAGATTCCa                                >  2:549737/1‑139 (MQ=255)                                                                                                 gTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTca                                                        <  2:183308/114‑1 (MQ=255)                                                                                                 gTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTca                                                        >  1:183308/1‑114 (MQ=255)                                                                                                  tGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTg                                                                                                         <  2:81062/64‑1 (MQ=255)                                                                                                  tGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTg                                                                                                         >  1:81062/1‑64 (MQ=255)                                                                                                  tGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGtt                                    <  1:929017/133‑1 (MQ=255)                                                                                                  tGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGtt                                    >  2:929017/1‑133 (MQ=255)                                                                                                           cGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGAt                     <  2:231508/139‑1 (MQ=255)                                                                                                           cGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGAt                     >  1:215848/1‑139 (MQ=255)                                                                                                                  aCCGTCTGGTAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCg              >  1:941265/1‑139 (MQ=255)                                                                                                                  aCCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTG‑TTCCATTCCTATTGATTTCAGCGa             <  1:603039/139‑1 (MQ=255)                                                                                                                   ccGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGa             <  2:1055947/139‑1 (MQ=255)                                                                                                                       cTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAAc         >  2:311597/1‑139 (MQ=255)                                                                                                                       cTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAAc         >  2:917449/1‑139 (MQ=255)                                                                                                                        tGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCaa                                                             >  2:65289/1‑86 (MQ=255)                                                                                                                        tGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCaa                                                             <  1:65289/86‑1 (MQ=255)                                                                                                                          gCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCt      <  2:215848/139‑1 (MQ=255)                                                                                                                              cTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGt  >  1:115318/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                TGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGT  >  NC_000913/2724312‑2724575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑