| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,742,962 | G→A | D194N (GAT→AAT) | yfiN → | putative membrane‑anchored diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,742,962 | 0 | G | A | 100.0% | 82.7 / NA | 27 | D194N (GAT→AAT) | yfiN | putative membrane‑anchored diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/11); total (16/11) | |||||||||||
AGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAG > NC_000913/2742830‑2743081 | aGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCg > 2:422347/1‑139 (MQ=255) gctcgcCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAAtttt > 1:930152/1‑139 (MQ=255) ttGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAAt > 1:1003210/1‑88 (MQ=255) ttGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAAt < 2:1003210/88‑1 (MQ=255) ttGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTACAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGgaa > 1:862687/1‑139 (MQ=255) tATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGgaagaa > 1:441442/1‑139 (MQ=255) ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCg > 1:97657/1‑139 (MQ=255) gCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTg < 2:854484/139‑1 (MQ=255) gCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTg > 2:5613/1‑139 (MQ=255) gCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTg > 2:453434/1‑139 (MQ=255) gCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTg > 1:902789/1‑139 (MQ=255) ccTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCtctc < 2:749220/139‑1 (MQ=255) tCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGa < 1:422347/139‑1 (MQ=255) tCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGa < 1:127414/139‑1 (MQ=255) gCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCaa < 1:5613/139‑1 (MQ=255) aTGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTa > 1:748436/1‑41 (MQ=255) aTGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTa < 2:748436/41‑1 (MQ=255) aTGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGa < 1:798674/139‑1 (MQ=255) ccTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGaa > 1:72179/1‑139 (MQ=255) ccTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGaa > 2:165158/1‑139 (MQ=255) ccTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGaa > 2:176505/1‑139 (MQ=255) ccTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAACTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGaa > 2:1050176/1‑139 (MQ=255) tGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAAt < 1:605153/139‑1 (MQ=255) gAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACAAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGTCTTCAACAGTCTGCTGGATGAAATGGAag < 1:705091/139‑1 (MQ=255) aGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAagag > 1:946898/1‑139 (MQ=255) gcgcTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGctc > 2:330576/1‑103 (MQ=255) gcgcTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGctc < 1:330576/103‑1 (MQ=255) | AGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAG > NC_000913/2742830‑2743081 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |