Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,814,218	C→T	*172* (TAG→TAA)	luxS ←	S‑ribosylhomocysteine lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,814,218	0	C	T	100.0%	133.5 / NA	43	*172* (TAG→TAA)	luxS	S‑ribosylhomocysteine lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/22);  total (21/22)

GTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCC  >  NC_000913/2814083‑2814349                                                                                                                                        |                                                                                                                                    gTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAg                                                                                                                                  <  2:487836/139‑1 (MQ=255)      gctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGAtgtg                                                                                                                             <  1:73866/139‑1 (MQ=255)       ctgGGGCTGGTATGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGc                                                                                                                            >  2:227766/1‑139 (MQ=255)                 tgtgGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCa                                                                                                                  >  1:23860/1‑139 (MQ=255)                     gTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACtt                                                                                                              >  1:852514/1‑139 (MQ=255)                      tttGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTtc                                                                                                             >  1:604854/1‑139 (MQ=255)                                     tttGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGcc                                                                                              >  2:367985/1‑139 (MQ=255)                                          cgcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttc                                                                                         <  2:443246/139‑1 (MQ=255)                                                              gTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGAGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTa                                                                     >  2:625057/1‑139 (MQ=255)                                                                  gcACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTc                                                                 <  2:984907/139‑1 (MQ=255)                                                                       aaGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGTTGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGtt                                                            <  2:77825/139‑1 (MQ=255)                                                                               cTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATg                                                    >  1:287882/1‑139 (MQ=255)                                                                               cTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATg                                                    >  2:116479/1‑139 (MQ=255)                                                                                 aaGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCt                                                  <  2:23860/139‑1 (MQ=255)                                                                                  aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCt                                                  <  2:296723/138‑1 (MQ=255)                                                                                  aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCt                                                  >  1:296723/1‑138 (MQ=255)                                                                                  aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTa                                                 >  1:180507/1‑139 (MQ=255)                                                                                  aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTa                                                 >  2:320787/1‑139 (MQ=255)                                                                                  aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTGGATGCGTACGTCACGTTCCAGAATGCTa                                                 >  1:634339/1‑139 (MQ=255)                                                                                   gCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTAc                                                <  2:852514/139‑1 (MQ=255)                                                                                       gTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAg                                                                                                                                  <  2:1001520/53‑1 (MQ=255)                                                                                       gTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAg                                                                                                                                  >  1:1001520/1‑53 (MQ=255)                                                                                        ttCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCa                                           >  1:634212/1‑139 (MQ=255)                                                                                         tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCaa                                          >  2:478990/1‑139 (MQ=255)                                                                                         tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCaa                                          >  1:235873/1‑139 (MQ=255)                                                                                                 aCTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCttc                                  <  2:634339/139‑3 (MQ=255)                                                                                                     gCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCtt                              >  2:786616/1‑139 (MQ=255)                                                                                                            tCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCaa                       <  1:625057/139‑1 (MQ=255)                                                                                                               attaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGa                    >  1:919574/1‑139 (MQ=255)                                                                                                                ttaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGTAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAg                   <  1:544413/139‑1 (MQ=255)                                                                                                                ttaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAg                   <  2:634212/139‑1 (MQ=255)                                                                                                                ttaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAg                   <  2:194977/139‑1 (MQ=255)                                                                                                                ttaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAg                   <  1:367985/139‑1 (MQ=255)                                                                                                                      gtgAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATc             <  2:281557/139‑1 (MQ=255)                                                                                                                          aGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGt         <  2:604854/139‑1 (MQ=255)                                                                                                                          aGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGt         <  2:906115/139‑1 (MQ=255)                                                                                                                              aGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAg                   <  2:155661/125‑1 (MQ=255)                                                                                                                              aGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAg                   >  1:155661/1‑125 (MQ=255)                                                                                                                                tttACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGc   <  1:227766/139‑1 (MQ=255)                                                                                                                                 ttACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGcc  >  2:570538/1‑139 (MQ=255)                                                                                                                                 ttACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGcc  <  2:919574/139‑1 (MQ=255)                                                                                                                                     tGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTg                                                                                  >  1:924453/1‑55 (MQ=255)                                                                                                                                     tGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTg                                                                                  <  2:924453/55‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    GTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCC  >  NC_000913/2814083‑2814349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑