Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,935,917	G→A	G112S (GGC→AGC)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,935,917	0	G	A	100.0%	68.0 / NA	22	G112S (GGC→AGC)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/9);  total (13/9)

CGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACA  >  NC_000913/2935796‑2936030                                                                                                                          |                                                                                                                   cGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGgc                                                                                                  <  1:921898/139‑1 (MQ=255)                             ggCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAg                                                                      >  2:926210/1‑139 (MQ=255)                             ggCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAg                                                                      >  2:111409/1‑139 (MQ=255)                               ccTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTCGCAGCGGCTCTGGCAGCt                                                                    <  2:585377/139‑1 (MQ=255)                                 tCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAg                                                                                  <  2:1029118/123‑1 (MQ=255)                                 tCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAg                                                                                  >  1:1029118/1‑123 (MQ=255)                                           ggTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGaaa                                                        <  2:129205/139‑1 (MQ=255)                                               aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCa                                                    >  1:981482/1‑139 (MQ=255)                                                       cTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCa                                            <  2:981482/139‑1 (MQ=255)                                                                 ggCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCAttt                                  >  2:225484/1‑139 (MQ=255)                                                                      tGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACgg                              >  1:409652/1‑139 (MQ=255)                                                                      tGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTa                                 >  2:400079/1‑135 (MQ=255)                                                                      tGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTa                                 <  1:400079/135‑1 (MQ=255)                                                                              tCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATcc                                                 >  1:784843/1‑111 (MQ=255)                                                                              tCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATcc                                                 <  2:784843/111‑1 (MQ=255)                                                                                   aTGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAgg                <  2:242359/139‑1 (MQ=255)                                                                                        tCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGcc           >  2:307318/1‑139 (MQ=255)                                                                                            aCCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATg       >  1:649213/1‑139 (MQ=255)                                                                                                 cccgAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCAt                                           <  2:995249/98‑1 (MQ=255)                                                                                                 cccgAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCAt                                           >  1:995249/1‑98 (MQ=255)                                                                                                 cccgAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGAcaca  >  2:989822/1‑139 (MQ=255)                                                                                                 cccgAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGAcaca  >  1:1051390/1‑139 (MQ=255)                                                                                                                          |                                                                                                                   CGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACA  >  NC_000913/2935796‑2936030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑