Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,129,036	G→A	*255* (TAG→TAA)	glcC →	glycolate‑inducible glc operon transcriptional repressor, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,129,036	0	G	A	100.0%	146.8 / NA	46	*255* (TAG→TAA)	glcC	glycolate‑inducible glc operon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/23);  total (23/23)

GCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCCGG  >  NC_000913/3128907‑3129166                                                                                                                                  |                                                                                                                                     gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTa                                                                                                                             >  2:279238/1‑139 (MQ=255) gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTa                                                                                                                             >  1:251727/1‑139 (MQ=255)      cGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCt                                                                                                                        <  2:738980/139‑1 (MQ=255)               cccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCAt                                                                                                               >  1:739526/1‑139 (MQ=255)               cccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCAt                                                                                                               >  1:581920/1‑139 (MQ=255)                        cagcaCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                                                      <  2:24002/139‑1 (MQ=255)                        cagcaCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                                                      <  2:948450/139‑1 (MQ=255)                              gcgATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGc                                                                                                 >  1:77441/1‑138 (MQ=255)                              gcgATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGc                                                                                                 <  2:77441/138‑1 (MQ=255)                                gATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑Tg                                                                                            >  1:294126/1‑139 (MQ=255)                                       tgCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCt                                                                                     <  2:440010/139‑1 (MQ=255)                                        gCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGtgtacggc                                                                                    >  1:112820/1‑132 (MQ=255)                                         cGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTcc                                                                                   <  2:581920/139‑1 (MQ=255)                                            aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTa                                                                                                     <  1:913786/120‑1 (MQ=255)                                            aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTa                                                                                                     >  2:913786/1‑120 (MQ=255)                                            aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGcc                                                                                >  1:553285/1‑139 (MQ=255)                                            aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGcc                                                                                >  1:511390/1‑139 (MQ=255)                                            aCCGTGAAAAAGAATCTCCACGATATCGAGCCGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGcc                                                                                >  1:956470/1‑139 (MQ=255)                                                tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCaa                                                                            >  2:857340/1‑139 (MQ=255)                                                tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCaa                                                                            >  2:700146/1‑139 (MQ=255)                                                tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCaa                                                                            >  2:125789/1‑139 (MQ=255)                                                tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGA‑GAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAAt                                                                           >  2:1053900/1‑139 (MQ=255)                                                 gAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAAt                                                                           <  1:112260/139‑1 (MQ=255)                                                         aTCTCCACGATATCGAGCCGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCa                                                                   <  2:956470/139‑1 (MQ=255)                                                          tctcCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAg                                                                  <  2:17334/139‑1 (MQ=255)                                                              cACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATg                                                              <  2:17888/139‑1 (MQ=255)                                                                      cGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCAt                                                      <  1:279238/139‑1 (MQ=255)                                                                             gAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTa                                               >  1:224790/1‑139 (MQ=255)                                                                              aaGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTAt                                              <  2:224790/139‑1 (MQ=255)                                                                                   caccaTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCt                                                                                                                        >  2:960407/1‑62 (MQ=255)                                                                                   caccaTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCt                                                                                                                        <  1:960407/62‑1 (MQ=255)                                                                                     ccaTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc                                       >  1:264957/1‑139 (MQ=255)                                                                                       aTTTGATTCGCTCGGCGGTGCCGCTGGATATGAACCTGAGTTAACTGGTATTACATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCACTGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa                                     <  2:511390/139‑1 (MQ=255)                                                                                       aTTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa                                     <  1:92041/139‑1 (MQ=255)                                                                                          tGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAg                                    <  2:112820/139‑1 (MQ=255)                                                                                                gCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTAtt                                             >  1:94818/1‑122 (MQ=255)                                                                                                gCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTAtt                                             <  2:94818/122‑1 (MQ=255)                                                                                                gCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATg                            >  2:530821/1‑139 (MQ=255)                                                                                                 cTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCa                                                       >  1:857489/1‑111 (MQ=255)                                                                                                 cTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAACGGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCa                                                       <  2:857489/111‑1 (MQ=255)                                                                                                      cggTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCa                                                                   <  1:935319/94‑1 (MQ=255)                                                                                                      cggTGCCGCTGGAGATGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCa                                                                   >  2:935319/1‑94 (MQ=255)                                                                                                                    aTGAACCTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATc        <  2:251727/139‑1 (MQ=255)                                                                                                                          cTGAGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCcgg  <  2:403834/139‑1 (MQ=255)                                                                                                                             aGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATg                                                              <  2:656926/76‑1 (MQ=255)                                                                                                                             aGTTAACTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATg                                                              >  1:656926/1‑76 (MQ=255)                                                                                                                                  |                                                                                                                                     GCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCT‑‑TGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGACATCTCCAGCGCATCGATCTTCCGG  >  NC_000913/3128907‑3129166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑