Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	308,135	A→G	L400L (TTG→CTG)	ecpC ←	ECP production outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	308,135	0	A	G	100.0%	133.7 / NA	38	L400L (TTG→CTG)	ecpC	ECP production outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/17);  total (21/17)

GCATATTTTGCAGGTTAACGTTGATCGCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCC  >  NC_000913/308021‑308269                                                                                                                   |                                                                                                                                       gCATATTTTGCAGGTTAACGTTGATCGCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGc                                                                                                                >  1:977821/1‑139 (MQ=255)       tttGCAGGTTAACGTTGATCGCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCa                                                                                                          >  2:138958/1‑139 (MQ=255)        ttGCAGGTTAACGTTGATCGCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCaa                                                                                                         <  2:238000/139‑1 (MQ=255)                            cccccccAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGt                                                                                     >  1:34624/1‑139 (MQ=255)                             ccccccAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTc                                                                                    >  1:623630/1‑139 (MQ=255)                                                 gACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCa                                                                <  2:661653/139‑1 (MQ=255)                                                    gggTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCg                                                             <  2:977821/139‑1 (MQ=255)                                                    gggTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCg                                                             <  2:933059/139‑1 (MQ=255)                                                      gTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTATTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGAt                                                           <  2:623630/139‑1 (MQ=255)                                                                ccGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGAt                                                           >  2:81298/1‑129 (MQ=255)                                                                ccGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGAt                                                           <  1:81298/129‑1 (MQ=255)                                                                ccGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGaaa                                                 >  2:216205/1‑139 (MQ=255)                                                                   ccTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCt                                              <  2:268754/139‑1 (MQ=255)                                                                     tGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTAc                                            <  2:123430/139‑1 (MQ=255)                                                                                 ccATATCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCt                                >  2:694334/1‑139 (MQ=255)                                                                                  cATATCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTa                                                                                                              >  1:138030/1‑60 (MQ=255)                                                                                  cATATCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTa                                                                                                              <  2:138030/60‑1 (MQ=255)                                                                                     atCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGc                                                                                                            >  1:472012/1‑59 (MQ=255)                                                                                     atCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGc                                                                                                            <  2:472012/59‑1 (MQ=255)                                                                                     atCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCa                            >  1:52867/1‑139 (MQ=255)                                                                                      tCCCGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCAc                           >  2:12553/1‑139 (MQ=255)                                                                                         cGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAg                                                               <  1:625303/100‑1 (MQ=255)                                                                                         cGTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAg                                                               >  2:625303/1‑100 (MQ=255)                                                                                          gTTGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCa                       >  1:536783/1‑139 (MQ=255)                                                                                           ttGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTAc                                            >  2:863485/1‑117 (MQ=255)                                                                                           ttGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTAc                                            <  1:863485/117‑1 (MQ=255)                                                                                           ttGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAg                      >  2:637188/1‑139 (MQ=255)                                                                                           ttGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTACGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAg                      <  1:937334/139‑1 (MQ=255)                                                                                           ttGCCGCCCAGCTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTC‑TTTTCCCGTTTTCCGACCAGCGATCCAGATGAAAGCTACCGCCCCATACCTGACACGCCAgt                     >  1:676068/1‑139 (MQ=255)                                                                                                      cTAAGCGTACTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGa           >  1:812782/1‑139 (MQ=255)                                                                                                               cTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAg                                                               <  1:113868/78‑1 (MQ=255)                                                                                                               cTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAg                                                               >  2:113868/1‑78 (MQ=255)                                                                                                               cTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGcc  >  2:197844/1‑139 (MQ=255)                                                                                                               cTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGcc  <  1:423084/139‑1 (MQ=255)                                                                                                               cTCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTACGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGcc  >  2:423084/1‑139 (MQ=255)                                                                                                                tCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGAc                                                                  <  2:1007543/74‑1 (MQ=255)                                                                                                                tCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGAc                                                                  >  1:1007543/1‑74 (MQ=255)                                                                                                                tCAGTGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTa                                             <  2:448729/95‑1 (MQ=255)                                                                                                                   |                                                                                                                                       GCATATTTTGCAGGTTAACGTTGATCGCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCC  >  NC_000913/308021‑308269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑