Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,220,190	G→A	A233T (GCT→ACT)	patA →	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,220,190	0	G	A	100.0%	118.5 / NA	37	A233T (GCT→ACT)	patA	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/20);  total (17/20)

CGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAA  >  NC_000913/3220059‑3220316                                                                                                                                    |                                                                                                                               cGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGt                                                                                                                         <  1:47030/139‑1 (MQ=255)           gAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGaa                                                                                                               <  1:657485/139‑1 (MQ=255)                 gACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGAtt                                                                                                         <  1:747037/139‑1 (MQ=255)                          cAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGaa                                                                                                >  2:696447/1‑139 (MQ=255)                             aCCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGt                                                                                                                         >  2:914539/1‑111 (MQ=255)                             aCCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGt                                                                                                                         <  1:914539/111‑1 (MQ=255)                                 tttATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGAtt                                                                                                         >  2:61769/1‑123 (MQ=255)                                 tttATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGAtt                                                                                                         <  1:61769/123‑1 (MQ=255)                                 tttATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCg                                                                                         >  1:1028516/1‑139 (MQ=255)                                         gTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTg                                                                                 >  1:811319/1‑139 (MQ=255)                                              tGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTgccgcc                                                                            <  1:696447/139‑1 (MQ=255)                                               gCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTgccgccg                                                                           >  2:785289/1‑139 (MQ=255)                                                   ggCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCgg                                                                       <  2:675948/139‑1 (MQ=255)                                                     cTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGt                                                                                                                         <  2:1007635/87‑1 (MQ=255)                                                     cTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGt                                                                                                                         >  1:1007635/1‑87 (MQ=255)                                                              tgtgCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCa                                                              >  2:1050927/1‑137 (MQ=255)                                                              tgtgCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCa                                                              <  1:1050927/137‑1 (MQ=255)                                                                tgCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAATCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgc                                                          <  2:739330/139‑1 (MQ=255)                                                                     tttGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGAtt                                                                                                         >  2:217655/1‑87 (MQ=255)                                                                     tttGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGAtt                                                                                                         <  1:217655/87‑1 (MQ=255)                                                                     tttGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCcgtac                                                     >  2:539440/1‑139 (MQ=255)                                                                                        tGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGAtt                                                                                                         <  1:7152/68‑1 (MQ=255)                                                                                        tGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGAtt                                                                                                         >  2:7152/1‑68 (MQ=255)                                                                                               ggCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAg                                    >  2:428476/1‑130 (MQ=255)                                                                                               ggCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAg                                    <  1:428476/130‑1 (MQ=255)                                                                                                gCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTaa                                                 >  1:714583/1‑116 (MQ=255)                                                                                                gCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTaa                                                 <  2:714583/116‑1 (MQ=255)                                                                                                    ttAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCa                           <  1:80932/134‑1 (MQ=255)                                                                                                    ttAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCa                           >  2:80932/1‑134 (MQ=255)                                                                                                            tGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCg              <  2:960555/139‑1 (MQ=255)                                                                                                            tGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCg              >  1:436573/1‑139 (MQ=255)                                                                                                            tGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCg              >  2:403618/1‑139 (MQ=255)                                                                                                             gCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGa             <  1:403618/139‑1 (MQ=255)                                                                                                             gCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGa             <  2:585818/139‑1 (MQ=255)                                                                                                              cAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgcc                                                         <  2:487710/94‑1 (MQ=255)                                                                                                              cAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgcc                                                         >  1:487710/1‑94 (MQ=255)                                                                                                                        ggTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACaaa  <  2:811319/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                               CGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAA  >  NC_000913/3220059‑3220316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑