Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,330,396	G→A	*478* (TAG→TAA)	dacB →	D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,330,396	0	G	A	100.0%	141.2 / NA	45	*478* (TAG→TAA)	dacB	D‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/21);  total (24/21)

CGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTA  >  NC_000913/3330263‑3330531                                                                                                                                       |                                                                                                                                        cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCG‑TTGTATAAAGATATTTATCAGAACAATTAATCaaa                                                                                                                                    >  2:181521/1‑139 (MQ=255)   agcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCaaaa                                                                                                                                   >  2:856043/1‑139 (MQ=255)      gggCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa                                                                                                                                <  1:2418/139‑1 (MQ=255)         cAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAc                                                                                                                             >  1:168264/1‑139 (MQ=255)           aCGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAccc                                                                                                                           <  2:108217/139‑1 (MQ=255)           aCGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAccc                                                                                                                           <  2:662314/139‑1 (MQ=255)                  gCGTTTGTGCAATATCTTTCTGGC‑ATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac                                                                                                                     >  1:852967/1‑137 (MQ=255)                  gCGTTTGTGCAATATCTTTCTGGC‑ATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac                                                                                                                     <  2:852967/137‑1 (MQ=255)                         tgCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCgg                                                                                                             >  2:264127/1‑139 (MQ=255)                                          tATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGAc                                                                                            >  1:20022/1‑139 (MQ=255)                                          tATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGAc                                                                                            >  2:89918/1‑139 (MQ=255)                                          tATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATC‑AAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACa                                                                                           >  1:88515/1‑139 (MQ=255)                                           aTGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACa                                                                                           >  1:500539/1‑139 (MQ=255)                                            tGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACaa                                                                                          <  1:30514/139‑1 (MQ=255)                                                      ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTCATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAg                                                                                        >  2:911682/1‑131 (MQ=255)                                                      ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAg                                                                                        <  1:911682/131‑1 (MQ=255)                                                      ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCttt                                                                                >  2:956062/1‑139 (MQ=255)                                                         gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGtt                                                                             >  1:259085/1‑139 (MQ=255)                                                           ggATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTa                                                                           >  1:308747/1‑139 (MQ=255)                                                                           cGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                                           >  1:834520/1‑139 (MQ=255)                                                                            gTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa                                                          <  2:834520/139‑1 (MQ=255)                                                                             tATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa                                                         <  2:168264/139‑1 (MQ=255)                                                                                    ttAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTa                                                  >  1:151389/1‑139 (MQ=255)                                                                                      aGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc                                                <  2:259085/139‑1 (MQ=255)                                                                                        tgcgtttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgc                                                                 <  1:33389/115‑1 (MQ=255)                                                                                        tgcgtttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgc                                                                 >  2:33389/6‑120 (MQ=255)                                                                                        tgcgtttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCg                                              <  2:308747/134‑1 (MQ=255)                                                                                         gcgtttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACa                                                                                           >  1:653502/5‑93 (MQ=255)                                                                                         gcgtttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACa                                                                                           <  2:653502/89‑1 (MQ=255)                                                                                                    aGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATc                                  >  2:370062/1‑139 (MQ=255)                                                                                                       cGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                                           <  2:302537/111‑1 (MQ=255)                                                                                                       cGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                                           >  1:302537/1‑111 (MQ=255)                                                                                                        gTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc                              <  2:500539/139‑1 (MQ=255)                                                                                                        gTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc                              <  1:89918/139‑1 (MQ=255)                                                                                                        gTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTGCAAAATCgcgc                              <  2:611632/139‑1 (MQ=255)                                                                                                        cgttGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc                              <  1:181521/137‑1 (MQ=255)                                                                                                                       tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTg               >  2:648104/1‑139 (MQ=255)                                                                                                                       tttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTg               >  1:97306/1‑139 (MQ=255)                                                                                                                        ttATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc              <  1:264127/139‑1 (MQ=255)                                                                                                                              gAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtct        >  2:116087/1‑139 (MQ=255)                                                                                                                               aaCAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtctc       >  1:864740/1‑139 (MQ=255)                                                                                                                                 cAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                    <  2:823769/108‑1 (MQ=255)                                                                                                                                 cAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                    >  1:823769/1‑108 (MQ=255)                                                                                                                                    ttAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTa  <  2:20022/139‑1 (MQ=255)                                                                                                                                    ttAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTa  <  2:864740/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        CGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTA  >  NC_000913/3330263‑3330531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑