Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,398,875 C→T M1M (GTG→ATG) †
*368* (TAG→TAA
mreD ←
mreC ←
cell wall structural complex MreBCD transmembrane component MreD
cell wall structural complex MreBCD transmembrane component MreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,398,8750CT100.0% 83.0 / NA 27M1M (GTG→ATG) †
*368* (TAG→TAA
mreD
mreC
cell wall structural complex MreBCD transmembrane component MreD
cell wall structural complex MreBCD transmembrane component MreC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/8);  total (19/8)

AATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCG  >  NC_000913/3398740‑3399002
                                                                                                                                       |                                                                                                                               
aaTACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTAt                                                                                                                              >  2:821987/1‑139 (MQ=255)
  tACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTg                                                                                                                            >  2:1010760/1‑139 (MQ=255)
       caaGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTc                                                                                                                       >  2:487986/1‑139 (MQ=255)
          gATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCg                                                                                                                    >  1:1002407/1‑139 (MQ=255)
                    acCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACgcgc                                                                                                          >  2:439480/1‑139 (MQ=255)
                     cccAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCa                                                                                                         >  1:794617/1‑139 (MQ=255)
                       cAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAgg                                                                                                       <  2:794617/139‑1 (MQ=255)
                          tttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg                                                                                                    <  1:1010760/139‑1 (MQ=255)
                          tttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg                                                                                                    <  1:487986/139‑1 (MQ=255)
                             ggCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTg                                                                                                 >  1:978875/1‑139 (MQ=255)
                                             ggTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcggc                                                                                 >  2:186998/1‑139 (MQ=255)
                                                 aTCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcggcggcg                                                                             <  2:978875/139‑1 (MQ=255)
                                                           ggCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGTTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTtg                                                                   >  2:425710/1‑139 (MQ=255)
                                                                gATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTa                                                                           >  2:398944/1‑126 (MQ=255)
                                                                gATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTa                                                                           <  1:398944/126‑1 (MQ=255)
                                                                            cAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTagcag                                                  <  2:1002407/139‑1 (MQ=255)
                                                                            cAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTagcag                                                  >  1:722173/1‑139 (MQ=255)
                                                                              gcgcAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAgcagca                                                >  2:707839/1‑139 (MQ=255)
                                                                                               agCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCgcagc                               >  1:57334/1‑139 (MQ=255)
                                                                                               agCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCgcagc                               >  1:331641/1‑139 (MQ=255)
                                                                                                     aTTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGgcgc                         <  2:331641/139‑1 (MQ=255)
                                                                                                                  ccTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCAttt            >  2:513299/1‑139 (MQ=255)
                                                                                                                           gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGcc   >  2:156456/1‑139 (MQ=255)
                                                                                                                           gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGcc   >  1:169711/1‑139 (MQ=255)
                                                                                                                            aTAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCg  >  2:117070/1‑139 (MQ=255)
                                                                                                                                cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTa                     <  1:556472/116‑1 (MQ=255)
                                                                                                                                cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTa                     >  2:556472/1‑116 (MQ=255)
                                                                                                                                       |                                                                                                                               
AATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCG  >  NC_000913/3398740‑3399002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: