Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,600,071	T→C	D238G (GAC→GGC)	rpoH ←	RNA polymerase, sigma 32 (sigma H) factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,600,071	0	T	C	100.0%	143.9 / NA	41	D238G (GAC→GGC)	rpoH	RNA polymerase, sigma 32 (sigma H) factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/21);  total (20/21)

CAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGTCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGC  >  NC_000913/3599943‑3600205                                                                                                                                 |                                                                                                                                       cagcaCGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCg                                                                                                                              <  1:1025493/139‑1 (MQ=255)   gcaCGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGtt                                                                                                                            >  1:529771/1‑139 (MQ=255)     aCGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCg                                                                                                                          >  1:97449/1‑139 (MQ=255)     aCGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCg                                                                                                                          >  2:145926/1‑139 (MQ=255)        cAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTCCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTcc                                                                                                                       <  2:504726/139‑1 (MQ=255)                     gcgTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATcg                                                                                                          >  2:44463/1‑139 (MQ=255)                                 aGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGAc                                                                                              <  1:614582/139‑1 (MQ=255)                                           cacGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTgccgc                                                                                    <  2:529771/139‑1 (MQ=255)                                              gCTCAGCGGAAACGCCGTAACGGTCAGCCATTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTg                                                                                 <  1:140785/139‑1 (MQ=255)                                                tCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGct                                                                               <  1:44463/139‑1 (MQ=255)                                                tCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGct                                                                               >  2:831628/1‑139 (MQ=255)                                                tCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGct                                                                               <  1:726907/139‑1 (MQ=255)                                                   gCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCtt                                                                            >  2:974005/1‑139 (MQ=255)                                                   gCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCtt                                                                            >  2:569621/1‑139 (MQ=255)                                                    cGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCt                                                                                  <  2:770791/132‑1 (MQ=255)                                                    cGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCt                                                                                  >  1:770791/1‑132 (MQ=255)                                                     ggAAACGCTGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTcc                                                                          <  1:627100/139‑1 (MQ=255)                                                        aaCGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAg                                                                       <  2:97449/139‑1 (MQ=255)                                                               tAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTatcat                                                                <  2:125245/139‑1 (MQ=255)                                                                       aGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATg                                                        >  1:55024/1‑139 (MQ=255)                                                                                   cAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAg                                            >  1:296796/1‑139 (MQ=255)                                                                                       gTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGttt                                                                                         >  1:361586/1‑90 (MQ=255)                                                                                       gTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGttt                                                                                         <  2:361586/90‑1 (MQ=255)                                                                                       gTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAg                                                                       >  2:723349/1‑108 (MQ=255)                                                                                       gTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAg                                                                       >  2:677013/1‑108 (MQ=255)                                                                                       gTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAg                                                                       <  1:723349/108‑1 (MQ=255)                                                                                       gTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAg                                        <  1:274860/139‑1 (MQ=255)                                                                                       gTGCACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAg                                                                       <  1:677013/108‑1 (MQ=255)                                                                                           aCTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAgatga                                    >  2:538760/1‑139 (MQ=255)                                                                                                     ttCGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGc                          >  2:601372/1‑139 (MQ=255)                                                                                                       cGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCt                            >  2:477525/1‑135 (MQ=255)                                                                                                       cGTCCAGCCAGCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCt                            <  1:477525/135‑1 (MQ=255)                                                                                                          ccagccagCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTccc                                                                         <  2:1023001/87‑1 (MQ=255)                                                                                                          ccagccagCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTccc                                                                         >  1:1023001/1‑87 (MQ=255)                                                                                                          ccagccagCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAgatg                                     <  1:885899/123‑1 (MQ=255)                                                                                                          ccagccagCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAgatg                                     >  2:885899/1‑123 (MQ=255)                                                                                                          ccagccagCGCGCACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATa                     <  1:601372/139‑1 (MQ=255)                                                                                                                      cACGGATGATGCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGc         >  2:208169/1‑139 (MQ=255)                                                                                                                           atgatgCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATaga                   <  1:857514/124‑1 (MQ=255)                                                                                                                           atgatgCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATaga                   >  2:857514/1‑124 (MQ=255)                                                                                                                             gatgCCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCggc  <  1:175801/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       CAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGTCCTGGCTGCGTTCGTCCAGACCCTGCATCGCGTCGGTCAGACGGTTTGCCGCCTGCTCTTCCCAGTTATCATCTTCAATGCCGTCGGCAAAGTTAGATGATTTATCCTGCAGATAGAGCACCGGAGCCATCGGC  >  NC_000913/3599943‑3600205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑