Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 349,847 T→C intergenic (+275/‑165) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913349,8470TC100.0% 91.8 / NA 33intergenic (+275/‑165)prpB/prpC2‑methylisocitrate lyase/2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/14);  total (19/14)

TGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAC  >  NC_000913/349712‑349981
                                                                                                                                       |                                                                                                                                      
tGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCgg                                                                                                                                     <  2:626732/139‑1 (MQ=21)
        cTTATCAGGCCTACTGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGCCGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACg                                                                                                                             <  1:646725/139‑1 (MQ=255)
           aTCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCAt                                                                                                                          <  1:521177/139‑1 (MQ=17)
                  cTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCg                                                                                                                   >  1:941483/1‑139 (MQ=17)
                     cGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATTCGCCGCTGACCCGACTTATCAGGCCTACGGGGCGCACCGAGCTGTAGGCCGGATAAGACGCATAGCGTCGCAt                                                                                                                >  2:846172/1‑139 (MQ=11)
                     cGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCAt                                                                                                                >  2:862950/1‑139 (MQ=17)
                          tGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGAc                                                                                                           >  2:683346/1‑139 (MQ=17)
                                      tAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTg                                                                                               >  2:376077/1‑139 (MQ=37)
                                      tAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTg                                                                                               >  1:739339/1‑139 (MQ=255)
                                         gTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATg                                                                                            >  1:611385/1‑139 (MQ=38)
                                                  gACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGa                                                                                   <  2:463209/139‑1 (MQ=255)
                                                     gCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAcgc                                                                                <  2:54011/139‑1 (MQ=37)
                                                        tAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGAc                                                                             >  2:9473/1‑139 (MQ=18)
                                                        tAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCCATGCCTGATGCGCCGCTGACGCGAc                                                                             >  2:241786/1‑139 (MQ=18)
                                                            gTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTAt                                                                         >  1:54011/1‑139 (MQ=17)
                                                                cATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAgg                                                                     >  1:180652/1‑139 (MQ=18)
                                                                            gATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCa                                                                       >  1:679437/1‑125 (MQ=17)
                                                                            gATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTAAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCa                                                                       <  2:679437/125‑1 (MQ=17)
                                                                             aTGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaa                                                        >  2:549217/1‑139 (MQ=21)
                                                                             aTGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaa                                                        >  2:392254/1‑139 (MQ=21)
                                                                                ccTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaactg                                                     <  1:549217/139‑1 (MQ=37)
                                                                                    aTGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTgaactgaact                                                 >  2:176400/1‑139 (MQ=21)
                                                                                     tGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGaactgaactg                                                <  2:180652/139‑1 (MQ=25)
                                                                                      gcgcCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGt                                               <  2:163617/139‑1 (MQ=37)
                                                                                          cgcTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCgg                                                                                                                                     >  1:731727/1‑49 (MQ=255)
                                                                                          cgcTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCgg                                                                                                                                     <  2:731727/49‑1 (MQ=255)
                                                                                             tGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCgg                                        <  2:739339/139‑1 (MQ=37)
                                                                                               aCGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGAt                                      <  1:862950/139‑1 (MQ=37)
                                                                                                            ggCCTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCg                         <  1:176400/139‑1 (MQ=18)
                                                                                                               cTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCg                      >  2:473945/1‑139 (MQ=18)
                                                                                                               cTACGGGGTGCACTGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCg                      <  1:473945/139‑1 (MQ=18)
                                                                                                                            tGAACTGTAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGAcaacaa         >  1:20242/1‑139 (MQ=21)
                                                                                                                                cTGTAGGCCGGATAAGACGCATACCGTCGCATCCGACAACCGCTGCCTGATGCGCCGCTCACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAAtctc     <  2:547894/139‑1 (MQ=255)
                                                                                                                                   tAGGCCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAc  >  1:1027277/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAC  >  NC_000913/349712‑349981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑