Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,963,829	C→T	R134H (CGC→CAC)	gpp ←	guanosine pentaphosphatase/exopolyphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,963,829	0	C	T	100.0%	93.2 / NA	30	R134H (CGC→CAC)	gpp	guanosine pentaphosphatase/exopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/14);  total (16/14)

AGATTACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGCGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCA  >  NC_000913/3963700‑3963963                                                                                                                                  |                                                                                                                                       aGATTACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATACACCACCAGGTGCTGATCgg                                                                                                                               >  2:685519/1‑139 (MQ=255)  gATTACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGc                                                                                                                              <  2:118696/139‑1 (MQ=255)            ggCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGcaccac                                                                                                                         <  2:851733/134‑1 (MQ=255)            ggCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGcaccac                                                                                                                         >  1:851733/1‑134 (MQ=255)            ggCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAgtg                                                                                                                    >  2:816333/1‑139 (MQ=255)                      cGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCaa                                                                                                          >  1:73330/1‑139 (MQ=255)                        ttCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATACACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACg                                                                                                        <  1:685519/139‑1 (MQ=255)                            agccagGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCt                                                                                                    <  2:935555/139‑1 (MQ=255)                              ccagGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGa                                                                                                  <  2:242599/139‑1 (MQ=255)                                   tGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATaatc                                                                                             >  2:212186/1‑137 (MQ=255)                                                            caacGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTg                                                                    <  2:22030/139‑1 (MQ=255)                                                                gAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCa                                                                >  1:1024840/1‑139 (MQ=255)                                                                 aGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGc                                                                                                       >  1:18543/1‑99 (MQ=255)                                                                 aGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGc                                                                                                       <  2:18543/99‑1 (MQ=255)                                                                 aGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCAc                                                               <  2:73330/139‑1 (MQ=255)                                                                 aGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCAc                                                               <  2:279783/139‑1 (MQ=255)                                                                                ccGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCg                                                                       <  1:451546/116‑1 (MQ=255)                                                                                ccGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCg                                                                       >  2:451546/1‑116 (MQ=255)                                                                                                          gCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATaa                      >  1:260750/1‑139 (MQ=255)                                                                                                          gCTCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATaa                      >  1:798571/1‑139 (MQ=255)                                                                                                            tCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCAc                                                               <  1:525456/96‑1 (MQ=255)                                                                                                            tCCGCCTATATCCACCACCAGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCAc                                                               >  2:525456/1‑96 (MQ=255)                                                                                                                          ccaccaGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGAc      >  2:911383/1‑139 (MQ=255)                                                                                                                           caccaGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTGACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCAGTGACg     >  1:655843/1‑139 (MQ=255)                                                                                                                           caccaGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCAc                                                               <  2:127206/81‑1 (MQ=255)                                                                                                                           caccaGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCAc                                                               >  1:127206/1‑81 (MQ=255)                                                                                                                            accaGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACgg    <  2:1012859/139‑1 (MQ=255)                                                                                                                              caGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCa  >  1:399469/1‑139 (MQ=255)                                                                                                                              caGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCa  >  1:953325/1‑139 (MQ=255)                                                                                                                              caGGTGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCa  <  1:909558/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       AGATTACGATCGGCAAAATAGCGTTCCAGCCAGGTGACGCAGCCCATCGACAGGCTGAACAACGAGGTGGTTTGTGCACCCGTGCCGGTTACCAGTTCAGTACTGGCTCCGCCTATATCCACCACCAGGCGCTGATCGGCACCACCAGTGGTGTGAGCAACGCCCTGATAAATCAGACGTGCTTCCTCTTCACCGCTGATCACCTGTACCGGACAACCGAGAATTTCCTGCGCTTTGGCAATAAAATCACCCGCATTGACGGCA  >  NC_000913/3963700‑3963963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑