| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,129,619 | C→T | G316G (GGC→GGT) | metB → | cystathionine gamma‑synthase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,129,619 | 0 | C | T | 100.0% | 90.3 / NA | 29 | G316G (GGC→GGT) | metB | cystathionine gamma‑synthase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (11/18); total (11/18) | |||||||||||
AAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGAC > NC_000913/4129489‑4129744 | aaaaaCTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTc < 2:145546/139‑1 (MQ=255) gTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGttt > 2:308456/1‑139 (MQ=255) tcgtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCgg < 2:68714/139‑1 (MQ=255) tcgtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCgg < 2:391440/139‑1 (MQ=255) cgtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGa > 1:105683/1‑139 (MQ=255) cgtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGa < 2:29408/139‑1 (MQ=255) cgtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCCTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGa > 1:643440/1‑139 (MQ=255) gtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGaa < 1:207764/139‑1 (MQ=255) gtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGaa < 1:379825/139‑1 (MQ=255) tCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGa < 2:190978/139‑1 (MQ=255) aGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGt > 2:203463/1‑139 (MQ=255) gggCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGACTgg < 1:906201/139‑1 (MQ=255) cATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGaaa > 1:963896/1‑139 (MQ=255) cAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCAcgccg < 2:105683/139‑1 (MQ=255) aaaaaGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAAc < 2:643440/139‑1 (MQ=255) gCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGac < 1:367202/139‑1 (MQ=255) gCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGac < 1:308456/139‑1 (MQ=255) tGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCg < 1:387854/52‑1 (MQ=255) tGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCg > 2:387854/1‑52 (MQ=255) tGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAggggg > 2:862381/1‑81 (MQ=255) tGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAggggg < 1:862381/81‑1 (MQ=255) tGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTa > 1:442289/1‑94 (MQ=255) tGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTa < 2:442289/94‑1 (MQ=255) gATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTc > 2:289477/1‑44 (MQ=255) gATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTc < 1:289477/44‑1 (MQ=255) aTGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGtgc < 2:888766/139‑1 (MQ=255) aGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATgg > 2:487649/1‑114 (MQ=255) aGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATgg < 1:487649/114‑1 (MQ=255) gtcgtTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGAc > 2:328053/1‑139 (MQ=255) | AAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGAC > NC_000913/4129489‑4129744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |