Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,158,617	T→C	L43P (CTG→CCG)	oxyR →	oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,158,617	0	T	C	100.0%	191.8 / NA	52	L43P (CTG→CCG)	oxyR	oxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/28);  total (24/28)

ATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGAC  >  NC_000913/4158483‑4158752                                                                                                                                       |                                                                                                                                        atggatAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGc                                                                                                                                     >  2:702522/1‑139 (MQ=255)   ggatAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGt                                                                                                                                   <  1:801086/139‑1 (MQ=255)     atAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGa                                                                                                                                 >  2:237420/1‑139 (MQ=255)           aaTATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGc                                                                                                                           >  1:81309/1‑139 (MQ=255)              atTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTgg                                                                                                                        >  1:926858/1‑139 (MQ=255)              atTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTgg                                                                                                                        >  2:1039471/1‑139 (MQ=255)              atTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTgg                                                                                                                        >  2:870177/1‑139 (MQ=255)               ttCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGa                                                                                                                       >  1:453180/1‑139 (MQ=255)               ttCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGa                                                                                                                       >  2:93940/1‑139 (MQ=255)               ttCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGa                                                                                                                       >  2:94539/1‑139 (MQ=255)               ttCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGa                                                                                                                       >  2:1015851/1‑139 (MQ=255)                tCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAg                                                                                                                      >  2:451281/1‑139 (MQ=255)                 cGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGc                                                                                                                     >  1:980160/1‑139 (MQ=255)                     aTCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGAc                                                                                                                 <  2:926858/139‑1 (MQ=255)                       cTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGt                                                                                                                                   <  2:995240/119‑1 (MQ=255)                       cTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGt                                                                                                                                   >  1:995240/1‑119 (MQ=255)                        ttGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAg                                                                                                              <  1:1039471/139‑1 (MQ=255)                         tGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAg                                                                                                              >  2:635015/1‑138 (MQ=255)                         tGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAg                                                                                                              <  1:635015/138‑1 (MQ=255)                          gAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGGAAATTCGTAAGCTGGAAGATGACCCGGGCGTGATGTTGCTGGAGCGGACCATcc                                                                                                            >  2:53701/1‑139 (MQ=255)                                cTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAg                                                                                                      <  1:1015851/139‑1 (MQ=255)                                cTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAg                                                                                                      >  2:1040705/1‑139 (MQ=255)                                 tggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgt                                                                                                     <  1:870177/139‑1 (MQ=255)                                  ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACTCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg                                                                                                    >  1:811589/1‑139 (MQ=255)                                   gtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtgt                                                                                                   >  2:177045/1‑139 (MQ=255)                                         ttGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCa                                                                                             >  2:905828/1‑139 (MQ=255)                                             cTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCa                                                                                         <  2:335808/139‑1 (MQ=255)                                                       ccATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtg                                                                               <  1:93940/139‑1 (MQ=255)                                                          ttttCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtgctg                                                                            >  2:340774/1‑139 (MQ=255)                                                              cGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCtgg                                                                        <  1:905828/139‑1 (MQ=255)                                                               ggCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtg                                                                               <  2:962499/131‑1 (MQ=255)                                                               ggCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtg                                                                               >  1:962499/1‑131 (MQ=255)                                                                   tGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCTAGGCGGGAATGCTGCTGGTGGAt                                                                   <  1:783926/139‑1 (MQ=255)                                                                   tGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGAt                                                                   <  1:368925/139‑1 (MQ=255)                                                                   tGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGAt                                                                   <  2:359799/139‑1 (MQ=255)                                                                   tGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGAt                                                                   <  2:848409/139‑1 (MQ=255)                                                                   tGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGAt                                                                   <  2:684898/139‑1 (MQ=255)                                                                     cGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGcc                                                                                                            <  2:951043/96‑1 (MQ=255)                                                                     cGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGcc                                                                                                            >  1:951043/1‑96 (MQ=255)                                                                     cGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCa                                                                 <  2:980160/139‑1 (MQ=255)                                                                        cAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGgc                                                              <  2:418084/139‑1 (MQ=255)                                                                                  ccACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGtg                                                    <  1:836940/139‑1 (MQ=255)                                                                                                  aCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAg                                    <  1:633801/139‑1 (MQ=255)                                                                                                   cGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGt                                   <  1:99728/139‑1 (MQ=255)                                                                                                             gCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGAt                                                                   >  2:871105/1‑97 (MQ=255)                                                                                                             gCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGAt                                                                   <  1:871105/97‑1 (MQ=255)                                                                                                                 aTTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATgg                     <  1:914093/139‑1 (MQ=255)                                                                                                                  ttCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGc                    <  1:177045/139‑1 (MQ=255)                                                                                                                   tCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCa                   <  2:811589/139‑1 (MQ=255)                                                                                                                         gCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGt                                              >  1:992782/1‑106 (MQ=255)                                                                                                                         gCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGt                                              <  2:992782/106‑1 (MQ=255)                                                                                                                                    aGCCGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGAc  <  2:453180/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        ATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGAC  >  NC_000913/4158483‑4158752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑