Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,377,189	C→T	H105H (CAC→CAT)  *178* (TAG→TAA)	sugE → blc ←	multidrug efflux system protein outer membrane lipoprotein cell division and growth lipocalin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,377,189	0	C	T	100.0%	122.9 / NA	39	H105H (CAC→CAT)  *178* (TAG→TAA)	sugE blc	multidrug efflux system protein outer membrane lipoprotein cell division and growth lipocalin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/20);  total (19/20)

TGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAG  >  NC_000913/4377056‑4377315                                                                                                                                      |                                                                                                                               tgtgGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCattaact                                                                                                                           >  2:493548/1‑139 (MQ=255)       cGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTAGTGGGGGTTATTGGTCTGAAACTCAGCACTCATTAACTACCAgg                                                                                                                     <  1:705878/139‑1 (MQ=255)                gcgcAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTAc                                                                                                            >  1:209458/1‑139 (MQ=255)                    aGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAg                                                                                                                      <  1:819924/125‑1 (MQ=255)                    aGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAg                                                                                                                      >  2:819924/1‑125 (MQ=255)                    aGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCaa                                                                                                        >  1:134421/1‑139 (MQ=255)                               ccATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACt                                                                                             <  1:444793/139‑1 (MQ=255)                               ccATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACt                                                                                             <  2:1029357/139‑1 (MQ=255)                                         cATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCTGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAAc                                                                                   >  1:911822/1‑139 (MQ=255)                                             gtgCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCtt                                                                               >  1:151313/1‑139 (MQ=255)                                              tgctgcTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTc                                                                              >  1:445430/1‑139 (MQ=255)                                              tgctgcTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTc                                                                              >  2:8740/1‑139 (MQ=255)                                                   ctCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCggg                                                                         <  1:267244/139‑1 (MQ=255)                                                        tGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTcgcg                                                                    >  1:649839/1‑139 (MQ=255)                                                            tCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCTGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTg                                                                <  2:911822/139‑1 (MQ=255)                                                                  aaCCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCa                                                          <  2:337232/139‑1 (MQ=255)                                                                      cGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCAtctc                                                      <  2:445430/139‑1 (MQ=255)                                                                         tGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTg                                                   >  2:1050645/1‑139 (MQ=255)                                                                               tGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCAc                                             <  2:685166/139‑1 (MQ=255)                                                                               tGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCAc                                             <  2:209458/139‑1 (MQ=255)                                                                               tGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCAc                                             >  2:629605/1‑139 (MQ=255)                                                                                     gTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTc                                       >  2:316063/1‑139 (MQ=255)                                                                                      tCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCaaa                                                                                                       <  1:867579/74‑1 (MQ=255)                                                                                      tCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCaaa                                                                                                       >  2:867579/1‑74 (MQ=255)                                                                                                    tATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTcc                                                     <  2:316317/110‑1 (MQ=255)                                                                                                    tATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTcc                                                     >  1:316317/1‑110 (MQ=255)                                                                                                    tATTGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGt                        >  2:792427/1‑139 (MQ=255)                                                                                                       tGGGGATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCg                     <  1:1050645/139‑1 (MQ=255)                                                                                                        ggggATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCgg                    <  2:151313/139‑1 (MQ=255)                                                                                                         gggATTATTCGTCTGTAACTCAGCGCTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACAGCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCggg                   <  2:631182/139‑1 (MQ=255)                                                                                                           gATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATATATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTAGTCAGAAATGGTTg                            <  1:641392/128‑1 (MQ=255)                                                                                                           gATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTg                            >  2:641392/1‑128 (MQ=255)                                                                                                           gATTATTGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGgag                 <  1:8740/139‑1 (MQ=255)                                                                                                            attattGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGgaga                <  1:106994/139‑1 (MQ=255)                                                                                                              tattGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGgaga                <  2:483212/137‑1 (MQ=255)                                                                                                              tattGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGgaga                >  1:483212/1‑137 (MQ=255)                                                                                                              tattGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGt              >  1:515115/1‑139 (MQ=255)                                                                                                                 tGGTCTGAAACTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATc           <  2:515115/139‑1 (MQ=255)                                                                                                                        aaaCTCAGCACTCATTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGt    >  2:448150/1‑139 (MQ=255)                                                                                                                          aCTCAGCACTCATCAACTACCAGGCTGCTGTACCCAAATAAATCTACTGACATCAAACCCTTCCCGTGTCCCGACTGCCAGCATCTCCTGTTTCACTTCGTGAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAg  >  2:174284/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                               TGTGGACGGGTATTGGCGCAGTCGGCGCGGCCATAACCGGCATTGTGCTGCTCGGTGAGTCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAG  >  NC_000913/4377056‑4377315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑