Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,392,463 A→G intergenic (+28/‑9) glyV → / → glyX tRNA‑Gly/tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,392,4630AG100.0% 90.9 / NA 28intergenic (+28/‑9)glyV/glyXtRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/13);  total (15/13)

CGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAG  >  NC_000913/4392329‑4392597
                                                                                                                                      |                                                                                                                                      
cGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGgccac                                                                                                                                    >  2:649160/1‑139 (MQ=255)
             aGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATa                                                                                                                       <  2:293843/139‑1 (MQ=255)
                            taCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGgccacc                                                                                                                                   >  1:102357/1‑112 (MQ=255)
                            taCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGgccacc                                                                                                                                   <  2:102357/112‑1 (MQ=255)
                              cgcgGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAACGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGct                                                                                                      <  1:649160/139‑2 (MQ=255)
                              cgcgGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCa                                                                                                      <  2:972112/139‑1 (MQ=255)
                                         cTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTa                                                                                                           <  1:359831/123‑1 (MQ=255)
                                         cTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTa                                                                                                           >  2:359831/1‑123 (MQ=255)
                                             gTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAg                                                                                                                      >  1:305419/1‑108 (MQ=255)
                                             gTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAg                                                                                                                      <  2:305419/108‑1 (MQ=255)
                                                   agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGc                                                                                                                     >  1:556327/1‑103 (MQ=255)
                                                   agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGc                                                                                                                     <  2:556327/103‑1 (MQ=255)
                                                      gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATa                                                                                                                       <  1:780235/98‑1 (MQ=255)
                                                      gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATa                                                                                                                       >  2:780235/1‑98 (MQ=255)
                                                                      gTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGCTGGGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGtt                                                              >  2:170653/1‑139 (MQ=255)
                                                                          gggTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTccc                                                          >  1:461930/1‑139 (MQ=255)
                                                                           ggTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGc                                                                                                                     <  2:951196/79‑1 (MQ=255)
                                                                           ggTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGc                                                                                                                     >  1:951196/1‑79 (MQ=255)
                                                                                cgAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCa                                                    >  1:635812/1‑139 (MQ=255)
                                                                                      cGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGa                                                                                                   >  1:1015375/1‑86 (MQ=255)
                                                                                      cGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGa                                                                                                   <  2:1015375/86‑1 (MQ=255)
                                                                                      cGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAAttt                                              >  2:271959/1‑139 (MQ=255)
                                                                                          tctcGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaaa                                          <  2:461930/139‑1 (MQ=255)
                                                                                                 tCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGt                                   <  1:271959/139‑1 (MQ=255)
                                                                                                          aaaaTTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGCCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAg                          >  2:542111/1‑139 (MQ=255)
                                                                                                                    aaGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAg                <  2:229974/139‑1 (MQ=255)
                                                                                                                       tgctgcAAAGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAg                                         >  2:564928/1‑111 (MQ=255)
                                                                                                                       tgctgcAAAGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAg                                         <  1:564928/111‑1 (MQ=255)
                                                                                                                         ctgcAAAGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGa           >  1:144732/1‑139 (MQ=255)
                                                                                                                                 cacaGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCa   >  1:924145/1‑139 (MQ=255)
                                                                                                                                 cacaGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAg  >  1:910044/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAG  >  NC_000913/4392329‑4392597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: