Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	361,924	G→A	intergenic (‑64/+2)	lacA ← / ← lacY	thiogalactoside acetyltransferase/lactose permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	361,924	0	G	A	100.0%	183.8 / NA	57	intergenic (‑64/+2)	lacA/lacY	thiogalactoside acetyltransferase/lactose permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/23);  total (34/23)

CGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTT  >  NC_000913/361792‑362059                                                                                                                                     |                                                                                                                                        cGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAg                                                                                                                                   <  2:688364/139‑1 (MQ=255) cGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAg                                                                                                                                   <  1:710089/139‑1 (MQ=255)  ggTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGc                                                                                                                                  >  1:977981/1‑139 (MQ=255)    tAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGa                                                                                                                                <  2:463738/139‑1 (MQ=255)                        tAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACgc                                                                                                            <  1:158100/139‑1 (MQ=255)                           aTAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACgcagc                                                                                                         >  2:161321/1‑139 (MQ=255)                           aTAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACgcagc                                                                                                         >  2:153368/1‑139 (MQ=255)                             aGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGcagcag                                                                                                       >  1:765733/1‑139 (MQ=255)                             aGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGcagcag                                                                                                       >  1:272262/1‑139 (MQ=255)                               cTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTgacg                                                                                                                >  1:848101/1‑128 (MQ=255)                               cTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTgacg                                                                                                                <  2:848101/128‑1 (MQ=255)                               cTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAggg                                                                                                     >  1:804767/1‑139 (MQ=255)                                  gCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGaaa                                                                                                  >  1:583752/1‑139 (MQ=255)                                            aTTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCgggccggg                                                                                        >  1:331970/1‑139 (MQ=255)                                             ttCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCgggccgggg                                                                                       <  2:15202/139‑1 (MQ=255)                                                tttCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCgggccggggccg                                                                                    <  2:395279/139‑1 (MQ=255)                                                 ttCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTgacga                                                                                                               <  2:588498/111‑1 (MQ=255)                                                 ttCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTgacga                                                                                                               >  1:588498/1‑111 (MQ=255)                                                  tCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCt                                                                                  >  1:17390/1‑139 (MQ=255)                                                  tCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCt                                                                                  >  2:270444/1‑139 (MQ=255)                                                      tCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGc                                                                              >  2:375768/1‑139 (MQ=255)                                                              aTGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAacac                                                                      <  1:159123/139‑1 (MQ=255)                                                              aTGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAacac                                                                      <  2:765733/139‑1 (MQ=255)                                                                      tGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTa                                                              >  1:957219/1‑139 (MQ=255)                                                                      tGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTa                                                              >  1:577030/1‑139 (MQ=255)                                                                           tCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACgcag                                                                                                          <  2:938877/90‑1 (MQ=255)                                                                           tCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACgcag                                                                                                          >  1:938877/1‑90 (MQ=255)                                                                           tCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTg                                                         <  2:692864/139‑1 (MQ=255)                                                                           tCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTg                                                         >  1:913180/1‑139 (MQ=255)                                                                           tCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTg                                                         >  1:188900/1‑139 (MQ=255)                                                                                  gTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCa                                                  >  2:396356/1‑139 (MQ=255)                                                                                         tatGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGccac                                           >  1:727264/1‑139 (MQ=255)                                                                                          atGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGccacc                                          <  1:548175/139‑1 (MQ=255)                                                                                           tGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGccacca                                         >  2:49941/1‑139 (MQ=255)                                                                                             ttGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGa                                                                          >  2:423927/1‑104 (MQ=255)                                                                                             ttGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGa                                                                          <  1:423927/104‑1 (MQ=255)                                                                                             ttGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGa                                       >  1:937151/1‑139 (MQ=255)                                                                                              tGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGAc                                      <  1:17049/139‑1 (MQ=255)                                                                                                gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTgacga                                                                                                               <  1:656763/64‑1 (MQ=255)                                                                                                gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTgacga                                                                                                               >  2:656763/1‑64 (MQ=255)                                                                                                gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAg                                                                                                 >  1:905782/1‑78 (MQ=255)                                                                                                gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAg                                                                                                 <  2:905782/78‑1 (MQ=255)                                                                                                gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGAccc                                    >  2:1012026/1‑139 (MQ=255)                                                                                                gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGAccc                                    >  2:264391/1‑139 (MQ=255)                                                                                                gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGAccc                                    >  1:916544/1‑139 (MQ=255)                                                                                                gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGAccc                                    >  2:824419/1‑139 (MQ=255)                                                                                                gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGAccc                                    >  1:144361/1‑139 (MQ=255)                                                                                                gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGAcc                                     >  2:384405/1‑138 (MQ=255)                                                                                                gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGAcc                                     <  1:384405/138‑1 (MQ=255)                                                                                                  cGGATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAg                                  >  1:728531/1‑139 (MQ=255)                                                                                                    gATAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCa                                <  2:728531/139‑1 (MQ=255)                                                                                                     aTAAGGCGCTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCAc                               >  2:149122/1‑139 (MQ=255)                                                                                                          gcgcTCGCGCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGAt                          >  2:341623/1‑139 (MQ=255)                                                                                                             cTCGCGACGCTTCCGACATTGATTACTTAAGCCAGGTCCTTCACCTGACGACGCAGTAGGGAAAGCGGGACGGGGCCGGTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCCCCACCAGAACCAGCACCAGATCAg                       <  2:384842/139‑1 (MQ=255)                                                                                                               cgcgCCGCATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAgcg                     <  2:870107/139‑1 (MQ=255)                                                                                                                    cgcATCCGACATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTg                <  1:49941/139‑1 (MQ=255)                                                                                                                            aCATTGATTACTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGa        <  2:17390/139‑1 (MQ=255)                                                                                                                              attgattaCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATg      <  2:804767/139‑1 (MQ=255)                                                                                                                              attgattaCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATg      <  1:270444/139‑1 (MQ=255)                                                                                                                                tgattaCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCt    <  2:577030/139‑1 (MQ=255)                                                                                                                                tgattaCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCt    <  1:824419/139‑1 (MQ=255)                                                                                                                                  attaCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCttt  >  1:652683/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                        CGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTT  >  NC_000913/361792‑362059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑