Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,490,141 C→T *333* (TAG→TAA idnR ← transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,490,1410CT100.0% 148.4 / NA 46*333* (TAG→TAAidnRtranscriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/25);  total (21/25)

CCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGC  >  NC_000913/4490005‑4490275
                                                                                                                                        |                                                                                                                                      
ggttagccattcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTa                                                                                                                                      <  2:566518‑M2/17‑1 (MQ=255)
  ttagccattcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTaaa                                                                                                                                    >  2:822289‑M2/121‑139 (MQ=255)
          tcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTg                                                                                                                            <  2:853791‑M2/27‑1 (MQ=255)
          tcccggcagtaaaaacagttctctgtccggcgtgctggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtcctgcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTg                                                                                                                            <  2:248124‑M2/27‑1 (MQ=255)
                   taaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATaa                                                                                                                   >  1:666989‑M2/104‑139 (MQ=255)
                            ttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAc                                                                                                          >  2:393884‑M2/95‑139 (MQ=255)
                                 tgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                     >  2:500262‑M2/90‑139 (MQ=255)
                                   tccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGa                                                                                                      <  2:974480‑M2/49‑1 (MQ=255)
                                   tccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGa                                                                                                      >  1:974480‑M2/88‑136 (MQ=255)
                                      ggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                <  2:760927‑M2/55‑1 (MQ=255)
                                      ggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                <  1:393884‑M2/55‑1 (MQ=255)
                                      ggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                <  1:500262‑M2/55‑1 (MQ=255)
                                      ggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                <  1:746702‑M2/55‑1 (MQ=255)
                                      ggcgtgcggacaattaacagcggttcacttatgagatatcctccagtttcctgcctggagaataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGTCGTGATAAATCTGATACCCGAGGTCaa                                                                                                <  2:976867‑M2/55‑1 (MQ=255)
                                              ggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAACAACTTATAGCGTGTTGCCGTGACAAATCTGATACCCGAGGTCAACAGtgttg                                                                                        <  2:610487‑M2/63‑1 (MQ=255)
                                                          gggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCg                                                                            <  2:174869‑M2/75‑1 (MQ=255)
                                                               cactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTAtt                                                                       >  1:27546‑M2/60‑139 (MQ=255)
                                                                    atgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTaa                                                                  >  1:939583‑M2/55‑139 (MQ=255)
                                                                    atgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTaa                                                                  >  2:740589‑M2/55‑139 (MQ=255)
                                                                       agatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGCGATCGTTATTTTTAAttt                                                               >  1:860895‑M2/52‑139 (MQ=255)
                                                                              ctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGcc                                                                                                                          <  1:158844‑M2/29‑1 (MQ=255)
                                                                              ctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGcc                                                                                                                          >  2:158844‑M2/45‑73 (MQ=255)
                                                                                 cagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                     <  1:462606‑M2/50‑1 (MQ=255)
                                                                                 cagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                     >  2:462606‑M2/42‑91 (MQ=255)
                                                                                 cagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACa                                                                                              <  1:483495‑M2/57‑1 (MQ=255)
                                                                                 cagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACa                                                                                              >  2:483495‑M2/42‑98 (MQ=255)
                                                                                            ctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGggcgg                                          <  1:822289‑M2/109‑1 (MQ=255)
                                                                                                agtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCAt                                      >  1:916063‑M2/27‑139 (MQ=255)
                                                                                                agtataggcaacgtccggcaaagagaGAAGAGAAAAGAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCTAGGTCAACAGAGTTGTGGCTGTGATCGTTATTTTTAATTTTCCTCAGCAACATTTGGGCGGCCAt                                      >  2:634553‑M2/27‑139 (MQ=255)
                                                                                                 gtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATc                                     <  2:939583‑M2/114‑1 (MQ=255)
                                                                                                     aggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGAc                                 <  2:510129‑M2/118‑1 (MQ=255)
                                                                                                     aggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGAc                                 <  2:666989‑M2/118‑1 (MQ=255)
                                                                                                         aacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAat                             >  2:583208‑M2/18‑139 (MQ=255)
                                                                                                          acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAata                            >  1:179307‑M2/17‑139 (MQ=255)
                                                                                                          acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAata                            >  2:968137‑M2/17‑139 (MQ=255)
                                                                                                            gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                           >  1:755420‑M2/15‑42 (MQ=255)
                                                                                                            gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                           <  2:755420‑M2/28‑1 (MQ=255)
                                                                                                              ccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAgtg                                                                                           <  2:324745‑M2/60‑1 (MQ=255)
                                                                                                              ccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAgtg                                                                                           >  1:324745‑M2/13‑72 (MQ=255)
                                                                                                                   aaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACgg                   <  1:346579‑M2/132‑1 (MQ=255)
                                                                                                                    aagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACggg                  <  1:404374‑M2/133‑1 (MQ=255)
                                                                                                                            agagAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgttg                                                                                        >  2:484149/1‑61 (MQ=255)
                                                                                                                            agagAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgttg                                                                                        <  1:484149/61‑1 (MQ=255)
                                                                                                                                   aTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                                             <  1:1045742/49‑1 (MQ=255)
                                                                                                                                   aTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                                             >  2:1045742/1‑49 (MQ=255)
                                                                                                                                    tAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGc  <  1:968137/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
CCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGC  >  NC_000913/4490005‑4490275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: