Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,507,463	G→A	intergenic (+12/+3)	insO → / ← insI1	pseudogene, IS911 transposase B,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related/IS30 transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,507,463	0	G	A	100.0%	144.6 / NA	47	intergenic (+12/+3)	insO/insI1	pseudogene, IS911 transposase B,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related/IS30 transposase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/29);  total (18/29)

CTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAGATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGACAT  >  NC_000913/4507332‑4507595                                                                                                                                    |                                                                                                                                     cTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAAt                                                                                                                               <  1:353947/139‑1 (MQ=255)     gTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGt                                                                                                                           >  2:613333/1‑139 (MQ=255)                 aGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACAcc                                                                                                               <  1:357832/139‑1 (MQ=255)                    gCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCt                                                                                                            <  2:50756/139‑1 (MQ=255)                                  gCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCtt                                                                                                           <  2:155823/126‑1 (MQ=255)                                  gCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCtt                                                                                                           >  1:155823/1‑126 (MQ=255)                                             aaCACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTg                                                                                   <  2:901817/139‑1 (MQ=255)                                                 ccAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACt                                                                               >  2:460929/1‑139 (MQ=255)                                                  cAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACtt                                                                              <  1:252637/139‑1 (MQ=255)                                                   aGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTc                                                                             >  1:863114/1‑139 (MQ=255)                                                       cAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAgtg                                                                         <  1:613333/139‑1 (MQ=255)                                                          aTCGAAATACGTAAGCTGATGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCt                                                                                                            <  2:624000/101‑1 (MQ=255)                                                          aTCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACAcc                                                                                                               <  1:976423/98‑1 (MQ=255)                                                          aTCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACAcc                                                                                                               >  2:976423/1‑98 (MQ=255)                                                          aTCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCt                                                                                                            >  1:624000/1‑101 (MQ=255)                                                          aTCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCt                                                                      >  1:202916/1‑139 (MQ=255)                                                          aTCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCt                                                                      >  2:1021422/1‑139 (MQ=255)                                                             gAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTtc                                                                   <  1:926627/139‑1 (MQ=255)                                                                   cGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTc                                                             <  2:202916/139‑1 (MQ=255)                                                                        gCTGAGGAAAAAGCTACAACGGATAGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCtgttg                                                        >  2:170355/1‑139 (MQ=255)                                                                            aGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTa                                                    >  2:323932/1‑139 (MQ=255)                                                                                  aaGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGag                                              <  1:460929/139‑1 (MQ=255)                                                                                   aGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGagc                                             >  1:888024/1‑139 (MQ=255)                                                                                    gCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGagca                                            <  1:867939/139‑1 (MQ=255)                                                                                          aCGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAAcc                                      <  2:888024/139‑1 (MQ=255)                                                                                          aCGGATAGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAAcc                                      <  1:170355/139‑1 (MQ=255)                                                                                             gATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGa                                   >  2:132619/1‑139 (MQ=255)                                                                                              aTTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCaa                                                                                                                                >  2:155593/1‑45 (MQ=255)                                                                                              aTTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCaa                                                                                                                                <  1:155593/45‑1 (MQ=255)                                                                                              aTTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAAc                                       <  1:1016259/134‑1 (MQ=255)                                                                                              aTTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAAc                                       >  2:1016259/1‑134 (MQ=255)                                                                                              aTTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGAt                                  <  2:863114/139‑1 (MQ=255)                                                                                               ttGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGAt                                  >  1:960863/1‑138 (MQ=255)                                                                                               ttGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGAt                                  <  2:960863/138‑1 (MQ=255)                                                                                                tGAAATGGATAATGAAATATTAAACATGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCTGTCTGTTGTTTAGCTGAGCAGCAACCAGATCt                                <  2:739287/139‑1 (MQ=14)                                                                                                   aaTGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGt                             <  1:323932/139‑1 (MQ=255)                                                                                                     tGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTc                           <  2:822683/139‑1 (MQ=255)                                                                                                       gagaATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCAt                         <  1:591957/139‑1 (MQ=255)                                                                                                           aTGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTg                     <  2:701494/139‑1 (MQ=255)                                                                                                            tGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGa                    >  1:1009934/1‑139 (MQ=255)                                                                                                                 tatTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGtata               <  2:993492/139‑1 (MQ=255)                                                                                                                 tatTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGtata               <  1:918537/139‑1 (MQ=255)                                                                                                                        aaGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGATc                           >  2:447610/1‑120 (MQ=255)                                                                                                                        aaGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGATc                           <  1:447610/120‑1 (MQ=255)                                                                                                                           gCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGa     <  2:919849/139‑1 (MQ=17)                                                                                                                             tACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGACa   >  1:308039/1‑139 (MQ=14)                                                                                                                              aCTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGACAt  <  2:1009934/139‑1 (MQ=11)                                                                                                                                    |                                                                                                                                     CTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAGATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGACAT  >  NC_000913/4507332‑4507595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑