Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,510,188	A→G	intergenic (+55/+502)	yjhV → / ← fecE	pseudogene, KpLE2 phage‑like element/ferric citrate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,510,188	0	A	G	100.0%	224.9 / NA	62	intergenic (+55/+502)	yjhV/fecE	pseudogene, KpLE2 phage‑like element/ferric citrate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/25);  total (37/25)

GTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCG  >  NC_000913/4510063‑4510318                                                                                                                              |                                                                                                                                   gTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGaaaaa                                                                                                                       >  1:637969/1‑139 (MQ=255)          gTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTc                                                                                                              <  2:767292/139‑1 (MQ=255)                cacCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAAc                                                                                                        >  1:411977/1‑139 (MQ=255)                   cGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTgg                                                                                                     >  1:1018818/1‑139 (MQ=255)                   cGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTgg                                                                                                     >  2:919654/1‑139 (MQ=255)                     aCTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGAt                                                                                                   >  1:708900/1‑139 (MQ=255)                          tGAAGATATCATTGATAACAATCGAACCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCa                                                                                              >  2:912283/1‑139 (MQ=255)                            aaGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAAt                                                                                            <  1:1035108/139‑1 (MQ=255)                                 atCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGt                                                                                       >  1:95786/1‑139 (MQ=255)                                 atCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGt                                                                                       >  2:953719/1‑139 (MQ=255)                                    aTTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTcc                                                                                    <  1:526436/139‑1 (MQ=255)                                      tGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGTACCTACCTAATTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAt                                                                                  <  2:708915/139‑1 (MQ=255)                                             aaTCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCt                                                                           <  2:711214/139‑1 (MQ=255)                                               tCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCttt                                                                         >  2:344703/1‑139 (MQ=255)                                               tCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCttt                                                                         >  1:632320/1‑139 (MQ=255)                                                  aTCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGtt                                                                                      <  1:318112/123‑1 (MQ=255)                                                  aTCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGtt                                                                                      >  2:318112/1‑123 (MQ=255)                                                  aTCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGa                                                                      >  1:768646/1‑139 (MQ=255)                                                  aTCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGa                                                                      >  2:378182/1‑139 (MQ=255)                                                        cAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACg                                                                <  1:810088/139‑1 (MQ=255)                                                          aCTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGtt                                                              <  1:215354/139‑1 (MQ=255)                                                                 tGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAAtt                                                                                           <  1:586136/103‑1 (MQ=255)                                                                 tGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAAtt                                                                                           >  2:586136/1‑103 (MQ=255)                                                                 tGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCt                                                       <  2:411977/139‑1 (MQ=255)                                                                   tataACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTc                                                        <  1:201194/136‑1 (MQ=255)                                                                   tataACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTc                                                        >  2:201194/1‑136 (MQ=255)                                                                      aaCGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCa                                                  <  2:95786/139‑1 (MQ=255)                                                                           ggCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAt                                             <  2:1011434/139‑1 (MQ=255)                                                                            gCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAt                                             >  1:483462/1‑138 (MQ=255)                                                                            gCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAt                                             <  2:483462/138‑1 (MQ=255)                                                                             cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAAt                                                                                                                      >  2:775015/1‑64 (MQ=255)                                                                             cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAAt                                                                                                                      <  1:775015/64‑1 (MQ=255)                                                                             cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAttt                                           >  1:967981/1‑139 (MQ=255)                                                                             cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAttt                                           >  2:147968/1‑139 (MQ=255)                                                                             cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAttt                                           >  1:252696/1‑139 (MQ=255)                                                                             cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAt                                             >  1:354690/1‑137 (MQ=255)                                                                             cTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAt                                             <  2:354690/137‑1 (MQ=255)                                                                                                cTGCACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCt                        >  2:123369/1‑139 (MQ=255)                                                                                                   cACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGccc                             >  2:565856/1‑131 (MQ=255)                                                                                                   cACCTACCTAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGccc                             <  1:565856/131‑1 (MQ=255)                                                                                                    acctacctAAGTAGTAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCAtt                    <  2:632320/139‑1 (MQ=255)                                                                                                             agtagtAGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGaa           >  2:176537/1‑139 (MQ=255)                                                                                                              gtagtaGCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAAc          <  1:344703/139‑1 (MQ=255)                                                                                                               tagtagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAAcc         <  2:252696/139‑1 (MQ=255)                                                                                                                  tagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATc                                                   >  2:598498/1‑94 (MQ=255)                                                                                                                  tagCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGc      >  1:73976/1‑139 (MQ=255)                                                                                                                  tagCAAACGCACGCTTTTTAGAAAAATCGATGGACAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATc                                                   <  1:598498/94‑1 (MQ=255)                                                                                                                   agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGt                                                               >  2:1016768/1‑81 (MQ=255)                                                                                                                   agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGt                                                               <  1:1016768/81‑1 (MQ=255)                                                                                                                   agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCa                                                  <  2:984911/94‑1 (MQ=255)                                                                                                                   agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCa                                                  >  1:984911/1‑94 (MQ=255)                                                                                                                   agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCt     >  2:62904/1‑139 (MQ=255)                                                                                                                   agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCt     >  1:154743/1‑139 (MQ=255)                                                                                                                   agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCt     >  1:147779/1‑139 (MQ=255)                                                                                                                   agCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCt     >  2:1002230/1‑139 (MQ=255)                                                                                                                    gCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTc    >  2:450010/1‑139 (MQ=255)                                                                                                                    gCAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTc    >  2:271266/1‑139 (MQ=255)                                                                                                                     cAAACGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACG‑TGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCg  >  1:366632/1‑139 (MQ=255)                                                                                                                         cGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGccc                             <  2:168077/109‑1 (MQ=255)                                                                                                                         cGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGccc                             >  1:168077/1‑109 (MQ=255)                                                                                                                         cGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCa                            <  1:924293/110‑1 (MQ=255)                                                                                                                         cGCACGCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCa                            >  2:924293/1‑110 (MQ=255)                                                                                                                              |                                                                                                                                   GTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCG  >  NC_000913/4510063‑4510318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑