Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,549,457	A→G	Y217C (TAC→TGC)	fimH →	minor component of type 1 fimbriae

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,549,457	0	A	G	100.0%	140.0 / NA	39	Y217C (TAC→TGC)	fimH	minor component of type 1 fimbriae
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/17);  total (22/17)

TAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACA  >  NC_000913/4549323‑4549579                                                                                                                                       |                                                                                                                           taatGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCctc                                                                                                                        >  1:633674/1‑139 (MQ=255)                           gttGCGATGTTTCTGCTCGTGATGTCACTGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGcac                                                                                                                <  1:260577/119‑1 (MQ=255)                           gcTGCGATGTTTCTGCTCGTGATGTCACTGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGcac                                                                                                                >  2:260577/1‑121 (MQ=255)                                  tGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                       <  2:633674/139‑1 (MQ=255)                                                      ccGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGtt                                                                   <  2:789524/139‑1 (MQ=255)                                                       cGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                       <  1:632084/118‑1 (MQ=255)                                                       cGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACAGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                       >  2:632084/1‑118 (MQ=255)                                                                    gACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAgg                                                     >  1:800633/1‑139 (MQ=255)                                                                                   gTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGt                                      >  2:298834/1‑139 (MQ=255)                                                                                    tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGt                                                 <  2:292243/127‑1 (MQ=255)                                                                                    tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGt                                                 >  1:292243/1‑127 (MQ=255)                                                                                    tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGtt                                     >  2:21209/1‑139 (MQ=255)                                                                                    tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGtt                                     >  1:91185/1‑139 (MQ=255)                                                                                    tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGtt                                     >  1:857932/1‑139 (MQ=255)                                                                                    tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGtt                                     >  1:743939/1‑139 (MQ=255)                                                                                    tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGtt                                     >  1:258219/1‑139 (MQ=255)                                                                                    tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGtt                                     >  1:1003222/1‑139 (MQ=255)                                                                                         aTTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGAcgc                                >  1:267994/1‑139 (MQ=255)                                                                                             ctctTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCaa                            <  1:21209/139‑1 (MQ=255)                                                                                                ttACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCCTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACgg                         <  2:743939/139‑1 (MQ=255)                                                                                                 tACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGt                        <  2:800633/139‑1 (MQ=255)                                                                                                            gtgCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTcc             >  1:663062/1‑139 (MQ=255)                                                                                                             tgCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCa            >  1:928708/1‑139 (MQ=255)                                                                                                             tgCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCa            <  2:20301/139‑1 (MQ=255)                                                                                                              gCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCggg                                                                                                 <  1:598436/53‑1 (MQ=255)                                                                                                              gCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCggg                                                                                                 >  2:598436/1‑53 (MQ=255)                                                                                                              gCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                       >  2:988599/1‑63 (MQ=255)                                                                                                              gCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                       <  1:988599/63‑1 (MQ=255)                                                                                                              gCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACgcgc                              >  2:113187/1‑120 (MQ=255)                                                                                                              gCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACgcgc                              <  1:113187/120‑1 (MQ=255)                                                                                                              gCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAg           >  2:106611/1‑139 (MQ=255)                                                                                                               cGAATAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACgcgc                              <  1:508630/119‑1 (MQ=255)                                                                                                               cGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACgcgc                              >  2:508630/1‑119 (MQ=255)                                                                                                                gAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCg         >  2:110364/1‑139 (MQ=255)                                                                                                                 aaaaGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGa        >  1:376625/1‑139 (MQ=255)                                                                                                                  aaaGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGaa       <  1:386026/139‑1 (MQ=255)                                                                                                                  aaaGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGaa       <  1:110364/139‑1 (MQ=255)                                                                                                                       cAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAaca  <  1:422692/139‑1 (MQ=255)                                                                                                                       cAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAaca  <  2:928708/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                           TAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACA  >  NC_000913/4549323‑4549579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑