Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,600,589	G→A	S129S (TCC→TCT)	dnaC ←	DNA biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,600,589	0	G	A	100.0%	93.5 / NA	30	S129S (TCC→TCT)	dnaC	DNA biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/11);  total (19/11)

AGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAAC  >  NC_000913/4600479‑4600724                                                                                                               |                                                                                                                                        agcagATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGc                                                                                                             >  1:486281/1‑139 (MQ=255)      aTCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACcgcg                                                                                                                              <  1:1040591/117‑1 (MQ=255)      aTCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACcgcg                                                                                                                              >  2:1040591/1‑117 (MQ=255)      aTCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGAtt                                                                                                        >  2:982426/1‑139 (MQ=255)      aTCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGAtt                                                                                                        >  2:518639/1‑139 (MQ=255)      aTCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGAtt                                                                                                        >  2:360498/1‑139 (MQ=255)        cAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTgc                                                                                                      >  1:916321/1‑139 (MQ=255)                     aTCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATgg                                                                                         >  1:805192/1‑139 (MQ=255)                       cGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGt                                                                                                                             <  1:363796/101‑1 (MQ=255)                       cGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGt                                                                                                                             >  2:363796/1‑101 (MQ=255)                                gttgttCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGt                                                                              <  1:322613/139‑1 (MQ=255)                                           cTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCTGATTGCCGCCGCCAGATGGTTTTTGCCGGCTCCTGGCTTAc                                                                   >  2:438783/1‑139 (MQ=255)                                           cTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGa                                                                                            <  1:654587/114‑1 (MQ=255)                                           cTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGa                                                                                            >  2:654587/1‑114 (MQ=255)                                           cTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTAc                                                                   >  1:888099/1‑139 (MQ=255)                                                         ccTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAg                                                     <  2:855818/139‑1 (MQ=255)                                                              aGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGc                                                >  2:598807/1‑139 (MQ=255)                                                                     tttCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTg                                         <  1:492484/139‑1 (MQ=255)                                                                             ccGACATAATATCGGCCACGGTGATGATCAATACAGTTTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCaaa                                 <  1:662278/139‑1 (MQ=255)                                                                             ccGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCaaa                                 >  1:333658/1‑139 (MQ=255)                                                                                         cGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATa                     <  2:486281/139‑1 (MQ=255)                                                                                           gCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACt                   >  1:629506/1‑139 (MQ=255)                                                                                           gCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACt                   >  2:808313/1‑139 (MQ=255)                                                                                                ggTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGgcgc              >  2:710917/1‑139 (MQ=255)                                                                                                    atgatCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCa                                   >  2:43033/1‑114 (MQ=255)                                                                                                    atgatCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCa                                   <  1:43033/114‑1 (MQ=255)                                                                                                    atgatCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCtt          <  2:347953/139‑1 (MQ=255)                                                                                                           aTACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCtt                            <  1:954087/114‑1 (MQ=255)                                                                                                           aTACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCtt                            >  2:954087/1‑114 (MQ=255)                                                                                                            tACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAAc  >  2:305424/1‑139 (MQ=255)                                                                                                               |                                                                                                                                        AGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAAC  >  NC_000913/4600479‑4600724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑