Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,634,581	A→G	Y244H (TAC→CAC)	rob ←	right oriC‑binding transcriptional activator, AraC family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,634,581	0	A	G	100.0%	191.6 / NA	54	Y244H (TAC→CAC)	rob	right oriC‑binding transcriptional activator, AraC family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/30);  total (24/30)

GGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGG  >  NC_000913/4634449‑4634715                                                                                                                                     |                                                                                                                                       ggATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGt                                                                                                                                  <  2:967778/139‑1 (MQ=255)   aTCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCa                                                                                                                                >  1:691596/1‑139 (MQ=255)             gcagTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCNGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCTGGATAAACTc                                                                                                                      >  1:881481/1‑139 (MQ=255)                gTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTg                                                                                                                   >  1:200961/1‑139 (MQ=255)                  tCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCa                                                                                                                 >  2:886104/1‑139 (MQ=255)                       gCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCg                                                                                                            <  1:1019049/139‑1 (MQ=255)                       gCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGACGCGTCAGGTTGAGCCTTGGCATGCAGGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCg                                                                                                            <  1:671569/139‑1 (MQ=255)                            gATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTTAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTcc                                                                                                       <  2:691596/139‑1 (MQ=255)                            gATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTcc                                                                                                       <  1:622300/139‑1 (MQ=255)                              ttAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCa                                                                                                     <  1:235681/139‑1 (MQ=255)                                aaTTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGa                                                                                                   <  1:1002877/139‑1 (MQ=255)                                aaTTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGa                                                                                                   <  2:469463/139‑1 (MQ=255)                                  ttGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGAcc                                                                                                 <  1:353025/139‑1 (MQ=255)                                     ggCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTc                                                                                              >  2:401715/1‑139 (MQ=255)                                       cGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCAt                                                                                            >  1:196751/1‑139 (MQ=255)                                            tcCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTa                                                                                       <  1:401715/139‑1 (MQ=255)                                                    tGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATcac                                                                               >  2:459989/1‑139 (MQ=255)                                                    tGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATcac                                                                               >  1:888043/1‑139 (MQ=255)                                                      gCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACat                                                                             >  1:179947/1‑139 (MQ=255)                                                              tGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTcgccg                                                                     <  1:639845/139‑1 (MQ=255)                                                                   ggTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTg                                                                >  1:738326/1‑139 (MQ=255)                                                                     tagtaTCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGt                                                                                        >  2:742774/1‑113 (MQ=255)                                                                     tagtaTCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGt                                                                                        <  1:742774/113‑1 (MQ=255)                                                                     tagtaTCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCt                                                                 >  2:885477/1‑136 (MQ=255)                                                                     tagtaTCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCt                                                                 <  1:885477/136‑1 (MQ=255)                                                                     tagtaTCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTgca                                                              >  2:332120/1‑139 (MQ=255)                                                                     tagtaTCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTgca                                                              >  2:255685/1‑139 (MQ=255)                                                                        taTCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTgcagca                                                           >  2:291354/1‑139 (MQ=255)                                                                             cTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCAcc                                                      >  2:1029126/1‑139 (MQ=255)                                                                             cTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCAcc                                                      >  1:475869/1‑139 (MQ=255)                                                                                     ccTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGccc                                              >  1:497808/1‑139 (MQ=255)                                                                                       tGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCATATATTCGCCGCCCTGCAGCATCACCGGATGCCCCg                                            >  1:638164/1‑139 (MQ=255)                                                                                        gACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGt                                           <  2:888043/139‑1 (MQ=255)                                                                                              tACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAAc                                                                                    >  1:69240/1‑92 (MQ=255)                                                                                              tACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAAc                                                                                    <  2:69240/92‑1 (MQ=255)                                                                                               aCGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACa                                    <  1:459989/139‑1 (MQ=255)                                                                                                           gTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGcc                        <  2:391517/139‑1 (MQ=255)                                                                                                           gTTGAGCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGcc                        <  2:678947/139‑1 (MQ=255)                                                                                                                gCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGc                                                >  2:228433/1‑110 (MQ=255)                                                                                                                gCATTGGCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGc                                                <  1:228433/110‑1 (MQ=255)                                                                                                                     ggCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTggg              >  1:313346/1‑139 (MQ=255)                                                                                                                     ggCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTggg              >  1:689793/1‑139 (MQ=255)                                                                                                                      gCATGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGc             <  1:603278/139‑1 (MQ=255)                                                                                                                         tGCACGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTaa          <  2:689793/139‑1 (MQ=255)                                                                                                                            aCGTTACGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCACAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAAcgc       <  2:78571/139‑1 (MQ=255)                                                                                                                             cGTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAAcgcg      <  2:738326/139‑1 (MQ=255)                                                                                                                              gTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCgg     <  2:638164/139‑1 (MQ=255)                                                                                                                              gTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCgg     <  2:200961/139‑1 (MQ=255)                                                                                                                              gTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCgg     <  1:426206/139‑1 (MQ=255)                                                                                                                              gTTCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCgg     <  1:925019/139‑1 (MQ=255)                                                                                                                                tCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCATACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCggtg   <  2:881481/139‑1 (MQ=255)                                                                                                                                tCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACAt                                   >  2:831716/1‑107 (MQ=255)                                                                                                                                tCCGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACAt                                   <  1:831716/107‑1 (MQ=255)                                                                                                                                 ccGTGTACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCggtgg  <  1:403389/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       GGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGG  >  NC_000913/4634449‑4634715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑