Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 47,776 | G→A | *177* (TAG→TAA) S3N (AGC→AAC) |
kefF → kefC → |
potassium‑efflux system ancillary protein for KefC, glutathione‑regulated, quinone oxidoreductase, FMN‑dependent potassium:proton antiporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 47,776 | 0 | G | A | 100.0% | 118.8 / NA | 39 | *177* (TAG→TAA) S3N (AGC→AAC) | kefF kefC | potassium‑efflux system ancillary protein for KefC, glutathione‑regulated, quinone oxidoreductase, FMN‑dependent potassium:proton antiporter |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/18); total (21/18) |
TCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGG > NC_000913/47643‑47903 | tCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATa > 1:820727/1‑139 (MQ=255) cGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGAt > 1:830590/1‑139 (MQ=255) cGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGAt > 1:81383/1‑139 (MQ=255) gCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCa > 2:575494/1‑139 (MQ=255) aaCTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgcg > 2:901628/1‑139 (MQ=255) cactgcacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcg < 2:830590/139‑1 (MQ=255) actgcacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc < 2:820727/139‑1 (MQ=255) ctgcacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCt > 1:526889/1‑139 (MQ=255) cacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGAt > 1:325483/1‑139 (MQ=255) acCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGAtt < 2:325483/139‑1 (MQ=255) ttGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCAt < 2:350887/139‑1 (MQ=255) ttGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCAt < 2:546987/139‑1 (MQ=255) gacgacGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCg < 2:526889/139‑1 (MQ=255) gacgacGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCg > 1:3404/1‑139 (MQ=255) gacgacGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCg > 1:211633/1‑139 (MQ=255) acgacgAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCgg > 1:662042/1‑139 (MQ=255) acgaAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTAc > 1:84701/1‑139 (MQ=255) cgaAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTa < 2:201571/137‑1 (MQ=255) cgaAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTa > 1:201571/1‑137 (MQ=255) aaCCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCt < 2:3404/139‑1 (MQ=255) cGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCt > 1:1037565/1‑139 (MQ=255) gCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGa < 2:965603/105‑1 (MQ=255) gCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGa > 1:965603/1‑105 (MQ=255) aGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCgg < 2:662042/139‑1 (MQ=255) cgcgTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTAc < 2:1026645/139‑1 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcg < 2:1013578/78‑1 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcg > 1:1013578/1‑78 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGt > 1:853515/1‑119 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGt < 2:853515/119‑1 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCg < 2:406550/139‑1 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCg > 1:840967/1‑139 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCg > 1:772427/1‑139 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCg < 1:575494/139‑1 (MQ=255) gctgGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCt > 1:80695/1‑100 (MQ=255) gctgGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCt < 2:80695/100‑1 (MQ=255) tGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGAt > 1:258272/1‑123 (MQ=255) tGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGAt < 2:258272/123‑1 (MQ=255) aggaggCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGcc < 1:901628/139‑1 (MQ=255) catcatGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTggggg > 1:96152/1‑139 (MQ=255) | TCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGG > NC_000913/47643‑47903 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |