Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 469,429 A→G K187E (AAG→GAG)  mdlA → putative multidrug ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913469,4290AG100.0% 136.1 / NA 38K187E (AAG→GAG) mdlAputative multidrug ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/19);  total (19/19)

ACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACC  >  NC_000913/469293‑469560
                                                                                                                                        |                                                                                                                                   
aCTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAg                                                                                                                                   <  2:181087/139‑1 (MQ=255)
  tGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTCGAGCt                                                                                                                                 >  2:118831/1‑139 (MQ=255)
    gTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTgg                                                                                                                               <  1:880643/139‑1 (MQ=255)
           gCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAggc                                                                                                                        >  1:92512/1‑139 (MQ=255)
                    tgtTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTc                                                                                                               <  2:776558/139‑1 (MQ=255)
                     gtTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGgcggcg                                                                                                                    <  2:485773/133‑1 (MQ=255)
                     gtTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGgcggcg                                                                                                                    >  1:485773/1‑133 (MQ=255)
                         aTTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTc                                                                                                          >  2:910751/1‑139 (MQ=255)
                           tATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCtt                                                                                                        <  2:126338/139‑1 (MQ=255)
                                tgTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGa                                                                                                   >  2:960809/1‑139 (MQ=255)
                                tgTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGa                                                                                                   <  2:365766/139‑1 (MQ=255)
                                   cTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCg                                                                                                >  1:854073/1‑139 (MQ=255)
                                             tAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGaa                                                                                      <  1:91470/139‑1 (MQ=255)
                                                    cAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTaa                                                                                                      <  2:626617/116‑1 (MQ=255)
                                                    cAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTaa                                                                                                      >  1:626617/1‑116 (MQ=255)
                                                     aGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCAc                                                                              >  2:95935/1‑139 (MQ=255)
                                                     aGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCAc                                                                              <  1:910751/139‑1 (MQ=255)
                                                     aGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCAc                                                                              >  2:272919/1‑139 (MQ=255)
                                                       ttGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCa                                                                            <  1:118831/139‑1 (MQ=255)
                                                         gACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGt                                                                          <  1:960809/139‑1 (MQ=255)
                                                                ttttCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCa                                                                   <  2:854073/139‑1 (MQ=255)
                                                                      cTGATGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTATTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCa                                                             >  2:679682/1‑139 (MQ=255)
                                                                             cGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCtt                                                      <  1:247533/139‑1 (MQ=255)
                                                                              gATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCttt                                                     >  1:643843/1‑139 (MQ=255)
                                                                                                     ttAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGc                              >  2:819537/1‑139 (MQ=255)
                                                                                                        aGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGtt                           >  1:532674/1‑139 (MQ=255)
                                                                                                          cgcAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGAcc                                                                                                 >  1:734627/1‑67 (MQ=255)
                                                                                                          cgcAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGAcc                                                                                                 <  2:734627/67‑1 (MQ=255)
                                                                                                          cgcAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTAt                         >  1:279983/1‑139 (MQ=255)
                                                                                                          cgcAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTAt                         >  2:216947/1‑139 (MQ=255)
                                                                                                             aaCGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCtt                                                      >  2:518858/1‑107 (MQ=255)
                                                                                                             aaCGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCtt                                                      <  1:518858/107‑1 (MQ=255)
                                                                                                             aaCGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCt                                                <  2:774374/113‑1 (MQ=255)
                                                                                                             aaCGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCt                                                >  1:774374/1‑113 (MQ=255)
                                                                                                                 gCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCcgc                  <  1:819537/139‑1 (MQ=255)
                                                                                                                  cGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCcgcg                 <  1:679682/139‑1 (MQ=255)
                                                                                                                      gCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATg             <  1:141613/139‑1 (MQ=255)
                                                                                                                                 aCGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATAcc  >  2:220204/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                   
ACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACC  >  NC_000913/469293‑469560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑