Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase, FAD/NAD(P)‑binding/leucine‑responsive global transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913932,3330A.93.8% 118.8 / 1.9 32intergenic (‑283/‑262)trxB/lrpthioredoxin reductase, FAD/NAD(P)‑binding/leucine‑responsive global transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base . (15/15);  total (16/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCA  >  NC_000913/932201‑932455
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tAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaa                                                                                                                      >  2:405699/1‑139 (MQ=255)
tAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaa                                                                                                                      >  2:234102/1‑139 (MQ=255)
  aaTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca                                                                                                                    <  1:234102/139‑3 (MQ=255)
  aaTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca                                                                                                                    <  1:398765/139‑3 (MQ=255)
   aTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATGGATGTTTTGCTTTGACAATCCCCAGGTGTTTTGCAAAAACATTCGAGGAAGaaaaaaacag                                                                                                                   >  2:534769/1‑136 (MQ=255)
       cccGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTAtt                                                                                                              >  2:649196/1‑139 (MQ=255)
                      gATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTtata                                                                                                        >  2:415925/1‑130 (MQ=255)
                      gATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTtata                                                                                                        <  1:415925/130‑1 (MQ=255)
                       aTTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAAc                                                                                              >  2:56647/1‑139 (MQ=255)
                        ttAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAAcc                                                                                             <  1:18081/139‑1 (MQ=255)
                        ttAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAAcc                                                                                             >  1:745186/1‑139 (MQ=255)
                           aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACcatg                                                                                          <  1:405699/139‑1 (MQ=255)
                                tgaATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGt                                                                                     <  2:766140/139‑1 (MQ=255)
                                  aaTAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTaa                                                                                   >  1:417414/1‑139 (MQ=255)
                                      aaCCCCCCTGTTAATGAATATCTGGCATGTTGTAATAAAAATCGATTGTTTGGTTTTACAATCCCCTTGTGGTTTGCGAAAACATTCGAGGAAAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATAc                                                                               <  1:317291/139‑1 (MQ=255)
                                       aCGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaacagt                                                                                                                  <  1:579713/104‑5 (MQ=255)
                                       aCGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaacagt                                                                                                                  >  2:579713/1‑100 (MQ=255)
                                       aCGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaa                                                                                                                      <  1:523604/100‑1 (MQ=255)
                                       aCGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaa                                                                                                                      >  2:523604/1‑100 (MQ=255)
                                          ccccTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAAAGAATTCTTATATGGGCATAACAATGAATGTAAATACAATg                                                                           >  2:317291/1‑139 (MQ=255)
                                                        tatCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGt                                                             <  2:761954/139‑1 (MQ=255)
                                                          tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca                                                                                                                    <  1:50496/83‑3 (MQ=255)
                                                          tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca                                                                                                                    >  2:50496/1‑81 (MQ=255)
                                                          tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaa                                                                                                                      <  2:266680/81‑1 (MQ=255)
                                                          tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaa                                                                                                                      >  1:266680/1‑81 (MQ=255)
                                                          tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATgcg                                                                                                    >  2:391415/1‑98 (MQ=255)
                                                          tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATgcg                                                                                                    <  1:391415/98‑1 (MQ=255)
                                                          tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACcatg                                                                                          <  1:830505/108‑1 (MQ=255)
                                                          tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACcatg                                                                                          >  2:830505/1‑108 (MQ=255)
                                                            tGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGaaa                                                         <  1:534769/139‑1 (MQ=255)
                                                              gCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca                                                                                                                    <  1:191740/79‑3 (MQ=255)
                                                              gCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca                                                                                                                    >  2:191740/1‑77 (MQ=255)
                                                                   ttgtCCTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCCTGCTAGTGAAATCTACGt                                                  <  1:551968/139‑1 (MQ=255)
                                                                           aaaaTCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATGACCATGCATGTAAATACCATGTTTACCGTGATAGTGAAATCTACGTATGGCGTg                                          >  2:551968/1‑139 (MQ=255)
                                                                              aTCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGAc                                       <  1:649196/139‑1 (MQ=255)
                                                                                               aCAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATg                      >  2:534465/1‑139 (MQ=255)
                                                                                                         ggTGTTTTGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTg            >  2:592506/1‑139 (MQ=255)
                                                                                                             ttttGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATgc                                                                                                     <  1:805502/46‑1 (MQ=39)
                                                                                                             ttttGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATgc                                                                                                     >  2:805502/1‑46 (MQ=39)
                                                                                                             ttatGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCg                                            <  1:83146/100‑1 (MQ=255)
                                                                                                             ttatGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCg                                            >  2:83146/4‑103 (MQ=255)
                                                                                                               ttGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAAt                                                        <  2:558387/89‑1 (MQ=255)
                                                                                                               ttGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAAt                                                        >  1:558387/1‑89 (MQ=255)
                                                                                                                tGCGAAAACATTCGAGGAAGATAAAAACAGTATTCTTATATGCGCATAACcatg                                                                                          <  2:497761/54‑1 (MQ=255)
                                                                                                                tGCGAAAACATTCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACcatg                                                                                          >  1:497761/1‑54 (MQ=255)
                                                                                                                          ttCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCa  <  2:614666/132‑1 (MQ=255)
                                                                                                                          ttCGAGGAAGAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCa  >  1:614666/1‑132 (MQ=255)
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TAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCA  >  NC_000913/932201‑932455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: