Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,325,247 (T)5→4 coding (502/1896 nt) yciQ → enhancer of membrane protein expression, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,325,2430T.100.0% 115.6 / NA 27coding (498/1896 nt)yciQenhancer of membrane protein expression, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (13/14);  total (13/14)

TGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCTT  >  NC_000913/1325113‑1325342
                                                                                                                                  |                                                                                                   
tGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTtctctc                                                                                            <  1:203806/139‑1 (MQ=255)
       aGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTtct                                                                                     >  1:73922/1‑139 (MQ=255)
                      tCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAg                                                                                         >  1:270333/1‑120 (MQ=255)
                      tCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAg                                                                                         <  2:270333/120‑1 (MQ=255)
                              aTTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCAttt                                                              >  2:205280/1‑139 (MQ=255)
                                tATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAatat                                                                              >  2:703525/1‑121 (MQ=255)
                                tATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAatat                                                                              <  1:703525/121‑1 (MQ=255)
                                         aaaaaTGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGa                                                   >  1:220557/1‑139 (MQ=255)
                                               gCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCtttt                                             <  1:205280/139‑1 (MQ=255)
                                                  ttCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTAc                                          <  1:426483/139‑1 (MQ=255)
                                                          tGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAg                                                                                         >  2:229256/1‑84 (MQ=255)
                                                          tGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAg                                                                                         <  1:229256/84‑1 (MQ=255)
                                                             gggggATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAgg                               >  2:797040/1‑139 (MQ=255)
                                                                ggATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCg                            <  1:797040/139‑1 (MQ=255)
                                                                  aTTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGac                          <  2:228278/139‑1 (MQ=255)
                                                                       gATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACata                     <  2:220557/139‑1 (MQ=255)
                                                                                aTCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCa                                                                                          >  2:842055/1‑61 (MQ=255)
                                                                                aTCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCa                                                                                          <  1:842055/61‑1 (MQ=255)
                                                                                       aCGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGac                          <  2:268624/118‑1 (MQ=255)
                                                                                       aCGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGac                          >  1:268624/1‑118 (MQ=255)
                                                                                          tGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCtt  >  2:339002/1‑139 (MQ=255)
                                                                                                       cTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTa                                           <  2:185815/85‑1 (MQ=255)
                                                                                                       cTGGCCGTTTGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTa                                           >  1:185815/1‑85 (MQ=255)
                                                                                                           ccGTTTGAAATTTATAAGACCCGTTTTCTCTCCAg                                                                                         >  1:58174/1‑35 (MQ=255)
                                                                                                           ccGTTTGAAATTTATAAGACCCGTTTTCTCTCCAg                                                                                         <  2:58174/35‑1 (MQ=255)
                                                                                                               ttGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACat                      >  2:283564/1‑98 (MQ=255)
                                                                                                               ttGAAATTTATAAGACCCGTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACat                      <  1:283564/98‑1 (MQ=255)
                                                                                                                                  |                                                                                                   
TGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGACCCGTTTTTCTCTCCAGTTCTCTAATATTGCGGGTAATCCATTTAGCGAAATCGATCTTTTTACCGGAGAAGAGGGCGACACATATCGTAATGGCCGCATCCTT  >  NC_000913/1325113‑1325342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: