Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,821,867 (A)8→7 intergenic (‑248/+51) chbB ← / ← osmE N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,821,8600A.100.0% 129.4 / NA 30intergenic (‑241/+58)chbB/osmEN,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (16/14);  total (16/14)

ATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAC  >  NC_000913/1821743‑1821980
                                                                                                                     |                                                                                                                        
ataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCg                                                                                                    >  2:416548/1‑139 (MQ=255)
         aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTGACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGGGCAATGGCCGGTTTCCgtt                                                                                           >  2:549236/1‑139 (MQ=255)
         aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCaaaaaaaccg                                                                                                                 >  1:111376/1‑115 (MQ=255)
         aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCaaaaaaaccg                                                                                                                 <  2:111376/118‑4 (MQ=255)
         aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                           >  2:428637/1‑139 (MQ=255)
         aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                           >  1:107049/1‑139 (MQ=255)
         aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                           >  1:319478/1‑139 (MQ=255)
              tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTAc                                                                                      >  1:69998/1‑139 (MQ=255)
              tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTAc                                                                                      >  2:355692/1‑139 (MQ=255)
                               tataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGaa                                                                     >  1:307556/1‑139 (MQ=255)
                                          tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTc                                                          >  1:200332/1‑139 (MQ=255)
                                           ctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                         <  1:428637/139‑1 (MQ=255)
                                             ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                       <  1:377620/139‑1 (MQ=255)
                                                  aCCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtg                                                  <  2:319478/139‑1 (MQ=255)
                                                       tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                               <  1:364470/89‑1 (MQ=255)
                                                       tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                               >  2:364470/1‑89 (MQ=255)
                                                              gACCCTCTTCACATTTCGGGCAAGTAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCTGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                      <  1:392315/139‑1 (MQ=255)
                                                              gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                      <  1:355692/139‑1 (MQ=255)
                                                              gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                      <  1:342933/139‑1 (MQ=255)
                                                                  ctctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                       <  1:289494/118‑1 (MQ=255)
                                                                  ctctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                       >  2:289494/1‑118 (MQ=255)
                                                                    ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                             >  1:772676/1‑126 (MQ=255)
                                                                    ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                             <  2:772676/126‑1 (MQ=255)
                                                                              cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                      >  2:823812/1‑123 (MQ=255)
                                                                              cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                      <  1:823812/123‑1 (MQ=255)
                                                                                 gCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGa                   >  2:588035/1‑139 (MQ=255)
                                                                                       aatTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAAGTCTGATAACCg             <  2:69998/139‑1 (MQ=255)
                                                                                               aaGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTAtt                                 >  2:694249/1‑111 (MQ=255)
                                                                                               aaGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTAtt                                 <  1:694249/111‑1 (MQ=255)
                                                                                                  aTAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc  <  2:644524/139‑1 (MQ=255)
                                                                                                             cTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                            <  2:430591/102‑1 (MQ=255)
                                                                                                             cTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                            >  1:430591/1‑102 (MQ=255)
                                                                                                                     |                                                                                                                        
ATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAC  >  NC_000913/1821743‑1821980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: