Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,869,022 (T)6→5 coding (68/1284 nt) yeaH → UPF0229 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,869,0170T.100.0% 92.0 / NA 22coding (63/1284 nt)yeaHUPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (11/11);  total (11/11)

CGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTT  >  NC_000913/1868886‑1869146
                                                                                                                                   |                                                                                                                                 
cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                            <  1:539206/139‑4 (MQ=255)
      gTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTta                                                                                                                     >  2:805184/1‑139 (MQ=255)
               agaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCa                                                                                                            >  1:327487/1‑139 (MQ=255)
                            caGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                               <  1:805184/139‑1 (MQ=255)
                            caGGCGTAGCAAACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                               <  2:816497/139‑1 (MQ=255)
                                         cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                  >  2:367822/1‑139 (MQ=255)
                                          aGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATAGCCAGCGTTTTTACGCCGTTATAAAGCTCAAATTAAACAGTCGATCTCCGAGGCCAt                                                                                 >  1:516938/1‑139 (MQ=255)
                                              ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                              <  2:538910/91‑2 (MQ=255)
                                              ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                              >  1:538910/1‑90 (MQ=255)
                                              ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                             >  2:76744/1‑139 (MQ=255)
                                                 aaaTGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAg                                                                          <  2:516938/139‑1 (MQ=255)
                                                              ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                            <  1:137101/77‑4 (MQ=255)
                                                              ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                            >  2:137101/1‑74 (MQ=255)
                                                                ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                              >  1:285655/1‑72 (MQ=255)
                                                                ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                              <  2:285655/73‑2 (MQ=255)
                                                                   atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTc                                                        >  1:812072/1‑139 (MQ=255)
                                                                             gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                              <  1:76744/139‑1 (MQ=255)
                                                                                    gACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATc                                          <  1:664820/136‑1 (MQ=255)
                                                                                    gACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATc                                          >  2:664820/1‑136 (MQ=255)
                                                                                      ccGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                     <  2:305364/91‑1 (MQ=255)
                                                                                      ccGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                     >  1:305364/1‑91 (MQ=255)
                                                                                       cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTaaa                                                                                                     <  2:323328/74‑1 (MQ=255)
                                                                                       cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTaaa                                                                                                     >  1:323328/1‑74 (MQ=255)
                                                                                          cGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                >  2:831774/1‑108 (MQ=255)
                                                                                          cGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                <  1:831774/108‑1 (MQ=255)
                                                                                                   ggCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                        <  1:367822/139‑1 (MQ=255)
                                                                                                       aaaaCAAAAGCATGGTGAATCGCCAGCGTTTTTATGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                    >  2:343617/1‑123 (MQ=255)
                                                                                                       aaaaCAAAAGCATGGTGAATCGCCAGCGTTTTTATGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                    <  1:343617/123‑1 (MQ=255)
                                                                                                                         aTCGCCAGCGTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGttt  <  2:812072/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                 
CGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTT  >  NC_000913/1868886‑1869146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: